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. 1964 Jan 1;20(1):57–74. doi: 10.1083/jcb.20.1.57

ADIPOSE TISSUE

Morphological Changes Associated with Lipid Mobilization

Joseph R Williamson 1
PMCID: PMC2106353  PMID: 14105216

Abstract

Morphological changes associated with mobilization of lipid were studied in epididymal adipose tissue from fasted and from alloxan diabetic rats. In both groups of animals a decrease in lipid content was accompanied by the formation of complex frond-like cytoplasmic processes and of loops and folds of basement membrane which extended from cell surfaces. These changes, evident after 1 day of fasting, increased in magnitude with increasing weight loss. As the lipid content of the cell decreased further, lipid-cytoplasmic interfaces became irregular and convoluted. Cytoplasmic microvesicles were prominent and appeared to be greatly increased in number. Rosette-like structures composed of microvesicles were observed in both lipid-depleted fat cells and endothelium. The interpretation of these changes and their physiological significance are discussed in terms of the physical and chemical properties of lipids and lipid metabolism. It is postulated that microvesicles may represent the mechanism of transport of free fatty acids in fat cells and in endothelium. Hypotheses are proposed and illustrated schematically for the mode of formation of microvesicular rosettes, for the mobilization and uptake of lipids by fat cells, and for the transport of lipids through endothelium.

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Selected References

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  1. ASHWORTH C. T., STEMBRIDGE V. A., SANDERS E. Lipid absorption, transport and hepatic assimilation with electron microscopy. Am J Physiol. 1960 Jun;198:1326–1328. doi: 10.1152/ajplegacy.1960.198.6.1326. [DOI] [PubMed] [Google Scholar]
  2. BARRNETT R. J., BALL E. G. Metabolic and ultrastructural changes induced in adipose tissue by insulin. J Biophys Biochem Cytol. 1960 Sep;8:83–101. doi: 10.1083/jcb.8.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BIERMAN E. L., GORDIS E., HAMLIN J. T., 3rd Heterogeneity of fat particles in plasma during alimentary lipemia. J Clin Invest. 1962 Dec;41:2254–2260. doi: 10.1172/JCI104684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BRAGDON J. H., GORDON R. S., Jr Tissue distribution of C14 after the intravenous injection of labeled chylomicrons and unesterified fatty acids in the rat. J Clin Invest. 1958 Apr;37(4):574–578. doi: 10.1172/JCI103640. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CASLEY-SMITH J. R., FLOREY H. W. The structure of normal small lymphatics. Q J Exp Physiol Cogn Med Sci. 1961 Jan;46:101–106. doi: 10.1113/expphysiol.1961.sp001502. [DOI] [PubMed] [Google Scholar]
  6. COURTICE G. C., MORRIS B. The exchange of lipids between plasma and lymph of animals. Q J Exp Physiol Cogn Med Sci. 1955 Apr;40(2):138–148. doi: 10.1113/expphysiol.1955.sp001105. [DOI] [PubMed] [Google Scholar]
  7. DALTON A. J., ZEIGEL R. F. A simplified method of staining thin sections of biolgical material with lead hydroxide for electron microscopy. J Biophys Biochem Cytol. 1960 Apr;7:409–410. doi: 10.1083/jcb.7.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DOLE V. P. The fatty acid pool in adipose tissue. J Biol Chem. 1961 Dec;236:3121–3124. [PubMed] [Google Scholar]
  9. FARQUHAR M. G. Origin and fate of secretory granules in cells of the anterior pituitary gland. Trans N Y Acad Sci. 1961 Feb;23:346–351. doi: 10.1111/j.2164-0947.1961.tb01361.x. [DOI] [PubMed] [Google Scholar]
  10. FAWCETT D. W. Observations on the cytology and electron microscopy of hepatic cells. J Natl Cancer Inst. 1955 Apr;15(5 Suppl):1475–1503. [PubMed] [Google Scholar]
  11. GORDON R. S., Jr, CHERKES A. Production of unesterified fatty acids from isolated rat adipose tissue incubated in vitro. Proc Soc Exp Biol Med. 1958 Jan;97(1):150–151. doi: 10.3181/00379727-97-23672. [DOI] [PubMed] [Google Scholar]
  12. GORDON R. S., Jr Interaction between oleate and the lipoproteins of human serum. J Clin Invest. 1955 Mar;34(3):477–484. doi: 10.1172/JCI103097. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MAJNO G., PALADE G. E. Studies on inflammation. 1. The effect of histamine and serotonin on vascular permeability: an electron microscopic study. J Biophys Biochem Cytol. 1961 Dec;11:571–605. doi: 10.1083/jcb.11.3.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MARCHESI V. T., FLOREY H. W. Electron micrographic observations on the emigration of leucocytes. Q J Exp Physiol Cogn Med Sci. 1960 Oct;45:343–348. doi: 10.1113/expphysiol.1960.sp001489. [DOI] [PubMed] [Google Scholar]
  15. MORRIS B., COURTICE F. C. The origin of chylomicrons in the cervical and hepatic lymph. Q J Exp Physiol Cogn Med Sci. 1956 Jul;41(3):341–348. doi: 10.1113/expphysiol.1956.sp001197. [DOI] [PubMed] [Google Scholar]
  16. PALAY S. L., KARLIN L. J. An electron microscopic study of the intestinal villus. I. The fasting animal. J Biophys Biochem Cytol. 1959 May 25;5(3):363–372. doi: 10.1083/jcb.5.3.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. PALAY S. L., KARLIN L. J. An electron microscopic study of the intestinal villus. II. The pathway of fat absorption. J Biophys Biochem Cytol. 1959 May 25;5(3):373–384. doi: 10.1083/jcb.5.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. RHODIN J. A. Fine structure of vascular walls in mammals with special reference to smooth muscle component. Physiol Rev Suppl. 1962 Jul;5:48–87. [PubMed] [Google Scholar]
  19. RHODIN J. A. The diaphragm of capillary endothelial fenestrations. J Ultrastruct Res. 1962 Apr;6:171–185. doi: 10.1016/s0022-5320(62)90052-7. [DOI] [PubMed] [Google Scholar]
  20. ROBINSON D. S., FRENCH J. E. Heparin, the clearing factor lipase, and fat transport. Pharmacol Rev. 1960 Jun;12:241–263. [PubMed] [Google Scholar]
  21. RODBELL M. The removal and metabolism of chylomicrons by adipose tissue in vitro. J Biol Chem. 1960 Jun;235:1613–1620. [PubMed] [Google Scholar]
  22. SHELDON H., HOLLENBERG C. H., WINEGRAD A. I. Observations on the morphology of adipose tissue. I. The fine structure of cells from fasted and diabetic rats. Diabetes. 1962 Sep-Oct;11:378–387. [PubMed] [Google Scholar]
  23. THOMAS W. A., O'NEAL R. M. Electron microscopy studies of butter and corn oil in jejunal mucosa. Arch Pathol. 1960 Feb;69:121–129. [PubMed] [Google Scholar]
  24. WASSERMANN F., McDONALD T. F. Electron microscopic investigation on the surface membrane structures of the fat-cell and of their changes during depletion of the cell. Z Zellforsch Mikrosk Anat. 1960;52:778–800. doi: 10.1007/BF00336627. [DOI] [PubMed] [Google Scholar]
  25. WILLIAMSON J. R., GRISHAM J. W. Electron microscopy of leukocytic margination and emigration in acute inflammation in dog pancreas. Am J Pathol. 1961 Aug;39:239–256. [PMC free article] [PubMed] [Google Scholar]
  26. WILLIAMSON J. R., LACY P. E., GRISHAM J. W. Ultrastructural changes in islets of the rat produced by tolbutamide. Diabetes. 1961 Nov-Dec;10:460–469. doi: 10.2337/diab.10.6.460. [DOI] [PubMed] [Google Scholar]

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