Skip to main content
PMC home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1964 Apr 1;21(1):105–114. doi: 10.1083/jcb.21.1.105

THE FATE OF DNA-CONTAINING PARTICLES PHAGOCYTIZED BY MAMMALIAN CELLS

Klaus Bensch 1, Gerald Gordon 1, Leonard Miller 1
PMCID: PMC2106423  PMID: 14154484

Abstract

Particulate DNA protein coacervates were digested immediately after being phagocytized by L strain fibroblasts in suspension culture. Enlargement of the phagocytotic vacuoles occurred simultaneously with a loss of the electron opacity of the phagocytized particles. Cytochemical reactions positive for non-specific esterase, acid phosphatase, and nucleoside phosphatase in the phagocytotic vacuoles provided additional evidence for the probability of complete hydrolysis of the phagocytized nucleoprotein.

Full Text

The Full Text of this article is available as a PDF (1.1 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARRNETT R. J. The fine structural localization of acetylcholinesterase at the myoneural junction. J Cell Biol. 1962 Feb;12:247–262. doi: 10.1083/jcb.12.2.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bensch K. G., King D. W. Incorporation of Heterologous Deoxyribonucleic Acid into Mammalian Cells. Science. 1961 Feb 10;133(3450):381–382. doi: 10.1126/science.133.3450.381. [DOI] [PubMed] [Google Scholar]
  3. DALES S., SIMINOVITCH L. The development of vaccinia virus in Earle's L strain cells as examined by electron microscopy. J Biophys Biochem Cytol. 1961 Aug;10:475–503. doi: 10.1083/jcb.10.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. ESSNER E. An electron microscopic study of erythrophagocytosis. J Biophys Biochem Cytol. 1960 Apr;7:329–334. doi: 10.1083/jcb.7.2.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FREEMAN J. A., SPURLOCK B. O. A new epoxy embedment for electron microscopy. J Cell Biol. 1962 Jun;13:437–443. doi: 10.1083/jcb.13.3.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GORDON G. B., MILLER L. R., BENSCH K. G. FIXATION OF TISSUE CULTURE CELLS FOR ULTRASTRUCTURAL CYTOCHEMISTRY. Exp Cell Res. 1963 Aug;31:440–443. doi: 10.1016/0014-4827(63)90024-7. [DOI] [PubMed] [Google Scholar]
  7. HESS R., PEARSE A. G. The histochemistry of indoxylesterase of rat kidney with special reference to its cathepsin-like activity. Br J Exp Pathol. 1958 Jun;39(3):292–299. [PMC free article] [PubMed] [Google Scholar]
  8. KARNOVSKY M. J. Simple methods for "staining with lead" at high pH in electron microscopy. J Biophys Biochem Cytol. 1961 Dec;11:729–732. doi: 10.1083/jcb.11.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KARRER H. E. Electron microscopic study of the phagocytosis process in lung. J Biophys Biochem Cytol. 1960 Apr;7:357–366. doi: 10.1083/jcb.7.2.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KARRER H. E. The ultrastructure of mouse lung: the alveolar macrophage. J Biophys Biochem Cytol. 1958 Nov 25;4(6):693–700. doi: 10.1083/jcb.4.6.693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MARCHESI V. T., BARRNETT R. J. The demonstration of enzymatic activity in pinocytic vesicles of blood capillaries with the electron microscope. J Cell Biol. 1963 Jun;17:547–556. doi: 10.1083/jcb.17.3.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. NAIDOO D., PRATT O. E. The development of adenosine 5'-phosphatase activity with the maturation of the rat cerebral cortex. Enzymologia. 1954 Mar 15;16(5):298–304. [PubMed] [Google Scholar]
  14. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. WACHSTEIN M., MEISEL E., FALCON C. Histochemistry of thiolacetic acid esterase: a comparison with nonspecific esterase with special regard to the effect of fixatives and inhibitors on intracellular localization. J Histochem Cytochem. 1961 May;9:325–339. doi: 10.1177/9.3.325. [DOI] [PubMed] [Google Scholar]
  17. WACHSTEIN M., MEISEL E. Histochemistry of hepatic phosphatases of a physiologic pH; with special reference to the demonstration of bile canaliculi. Am J Clin Pathol. 1957 Jan;27(1):13–23. doi: 10.1093/ajcp/27.1.13. [DOI] [PubMed] [Google Scholar]
  18. de DUVE [Localization of enzymes in the formed elements of the cells]. Bull Soc Chim Biol (Paris) 1960;42:11–29. [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES