Abstract
Electron micrographs were made of rotifers of known ages embedded in Vestopal and stained with lead hydroxide. In epithelial cells lining the gut of the rotifer, anatomical continuity is seen between the cell membrane, the cortical membranes, and the rough endoplasmic reticulum at the basal region of the cells. At their luminal surface, these cells possess a terminal web, and colloidal gold is taken up via food vacuoles at this surface. Such pinocytosis does not occur on the peripheral or distal surface (pseudocoelomic) of these cells. Exposure of rotifers to adenine inhibits the formation of cortical infoldings of the cell membrane and the formation of the rough reticulum, whereas the ribosomes and the Golgi apparatus appear to be unaffected. The suggestion is made that the rough reticulum may be derived from modifications of the cell membrane and that the rate of formation of the rough reticulum in adult and elderly rotifers is lower than in the actively growing animal.
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Selected References
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- ARONOW L. Reversal of adenine toxicity by pyrimidine mucleosides. Biochim Biophys Acta. 1961 Feb 12;47:184–185. doi: 10.1016/0006-3002(61)90846-0. [DOI] [PubMed] [Google Scholar]
- FINCK H. Epoxy resins in electron microscopy. J Biophys Biochem Cytol. 1960 Feb;7:27–30. doi: 10.1083/jcb.7.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ITO S., WINCHESTER R. J. The fine structure of the gastric mucosa in the bat. J Cell Biol. 1963 Mar;16:541–577. doi: 10.1083/jcb.16.3.541. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KARNOVSKY M. J. Simple methods for "staining with lead" at high pH in electron microscopy. J Biophys Biochem Cytol. 1961 Dec;11:729–732. doi: 10.1083/jcb.11.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KARRER H. E. Electron microscopic study of the phagocytosis process in lung. J Biophys Biochem Cytol. 1960 Apr;7:357–366. doi: 10.1083/jcb.7.2.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LANSING A. I., ROSENTHAL T. B. The relation between ribonucleic acid and ionic transport across the cell surface. J Cell Physiol. 1952 Oct;40(2):337–345. doi: 10.1002/jcp.1030400212. [DOI] [PubMed] [Google Scholar]
- Lansing A. I. Evidence for Aging as a Consequence of Growth Cessation. Proc Natl Acad Sci U S A. 1948 Jun;34(6):304–310. doi: 10.1073/pnas.34.6.304. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MILLINGTON P. F. COMPARISON OF THE THICKNESSES OF THE LATERAL WALL MEMBRANE AND THE MICROVILLUS MEMBRANE OF INTESTINAL EPITHELIAL CELLS FROM RAT AND MOUSE. J Cell Biol. 1964 Mar;20:514–517. doi: 10.1083/jcb.20.3.514. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PALAY S. L., KARLIN L. J. An electron microscopic study of the intestinal villus. I. The fasting animal. J Biophys Biochem Cytol. 1959 May 25;5(3):363–372. doi: 10.1083/jcb.5.3.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PUCHTLER H., LEBLOND C. P. Histochemical analysis of cell membranes and associated structures as seen in the intestinal epithelium. Am J Anat. 1958 Jan;102(1):1–31. doi: 10.1002/aja.1001020102. [DOI] [PubMed] [Google Scholar]
- YAMAMOTO T. On the thickness of the unit membrane. J Cell Biol. 1963 May;17:413–421. doi: 10.1083/jcb.17.2.413. [DOI] [PMC free article] [PubMed] [Google Scholar]