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. 1964 Dec 1;23(3):431–446. doi: 10.1083/jcb.23.3.431

ELECTRON MICROSCOPE STUDY OF SPERMIOGENESIS IN A FIRE-BRAT INSECT, THERMOBIA DOMESTICA PACK

I. Mature Spermatozoon

S R Bawa 1
PMCID: PMC2106548  PMID: 14245431

Abstract

The fine structure of the mature spermatozoon of the insect Thermobia domestica has been investigated. This flagellate spermatozoon is unique with respect to the relative positions of the centriole, nucleus, mitochondrial nebenkern derivatives, and acrosome along the length of the cell. The acrosome lies at the posterior end of the nucleus. Unlike spermatozoa of most animals, the Thermobia spermatozoon has a nucleus that reveals an unusual lamellar pattern of the material inside of it. This flagellate spermatozoon is also unusual in its tendency to intertwine with other spermatozoa, and during movement the intertwined portion of two (double) spermatozoa is always directed forwards. In the axial filament complex, one of the fibrils of each peripheral double fibril bears a hook-shaped process. An indistinct granular material is seen between the 9 outer coarse fibers. Cytoplasmic tubules, probably corresponding to the "microtubules" of other investigators (67), have been observed around the nucleus, mitochondrial nebenkern derivatives, axial filament complex, and acrosome. A description is given of a complex membrane system which surrounds and separates the main organelles of the cell from each other.

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Selected References

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  1. AFZELIUS B. A. The fine structure of the cilia from ctenophore swimming-plates. J Biophys Biochem Cytol. 1961 Feb;9:383–394. doi: 10.1083/jcb.9.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. AFZELIUS B. A. The fine structure of the sea urchin spermatozoa as revealed by the electron microscope. Z Zellforsch Mikrosk Anat. 1955;42(1-2):134–148. doi: 10.1007/BF00335087. [DOI] [PubMed] [Google Scholar]
  3. AFZELIUS B. Electron microscopy of the sperm tail; results obtained with a new fixative. J Biophys Biochem Cytol. 1959 Mar 25;5(2):269–278. doi: 10.1083/jcb.5.2.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BERNHARD W., DE HARVEN E. Etude au microscope électronique de l'ultrastructure du centriole chez les vertébrés. Z Zellforsch Mikrosk Anat. 1956;45(3):378–398. [PubMed] [Google Scholar]
  5. CAULFIELD J. B. Effects of varying the vehicle for OsO4 in tissue fixation. J Biophys Biochem Cytol. 1957 Sep 25;3(5):827–830. doi: 10.1083/jcb.3.5.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. COLWIN L. H., COLWIN A. L. Changes in the spermatozoon during fertilization in Hydroides hexagonus (Annelida). I. Passage of the acrosomal region through the vitelline membrane. J Biophys Biochem Cytol. 1961 Jun;10:231–254. doi: 10.1083/jcb.10.2.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DASS C. M., RIS H. Submicroscopic organization of the nucleus during spermiogenesis in the grasshopper. J Biophys Biochem Cytol. 1958 Jan 25;4(1):129–132. doi: 10.1083/jcb.4.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FAWCETT D. W. Intercellular bridges. Exp Cell Res. 1961;Suppl 8:174–187. doi: 10.1016/0014-4827(61)90347-0. [DOI] [PubMed] [Google Scholar]
  9. GATENBY J. B., MATHUR R. S. Position of the proximal centriole in flagellate spermatozoa. Nature. 1960 Mar 19;185:861–862. doi: 10.1038/185861a0. [DOI] [PubMed] [Google Scholar]
  10. GATENBY J. B. The electron microscopy of centriole, flagellum and cilium. J R Microsc Soc. 1961 Feb;79:299–317. doi: 10.1111/j.1365-2818.1961.tb05225.x. [DOI] [PubMed] [Google Scholar]
  11. GINSBURG A. S. Sperm-egg association and its relationship to the activation of the egg in salmonid fishes. J Embryol Exp Morphol. 1963 Mar;11:13–33. [PubMed] [Google Scholar]
  12. LANG N. J. AN ADDITIONAL ULTRASTRUCTURAL COMPONENT OF FLAGELLA. J Cell Biol. 1963 Dec;19:631–634. doi: 10.1083/jcb.19.3.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LEBLOND C. P. Distribution of periodic acid-reactive carbohydrates in the adult rat. Am J Anat. 1950 Jan;86(1):1–49. doi: 10.1002/aja.1000860102. [DOI] [PubMed] [Google Scholar]
  14. LEUCHTENBERGER C., SCHRADER F. The chemical nature of the acrosome in the male germ cells. Proc Natl Acad Sci U S A. 1950 Nov;36(11):677–683. doi: 10.1073/pnas.36.11.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LUFT J. H. Permanganate; a new fixative for electron microscopy. J Biophys Biochem Cytol. 1956 Nov 25;2(6):799–802. doi: 10.1083/jcb.2.6.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. NATH V., GUPTA B. L., MITTAL L. C., GATENBY J. B., MATHUR R. S. Position of the proximal centriole in flagellate spermatozoa. Nature. 1960 Jun 11;186:899–901. doi: 10.1038/186899a0. [DOI] [PubMed] [Google Scholar]
  18. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. RODE L. J., FOSTER J. W. Germination of bacterial spores by long-chain alkyl amines. Nature. 1960 Dec 24;188:1132–1134. doi: 10.1038/1881132b0. [DOI] [PubMed] [Google Scholar]
  20. SLAUTTERBACK D. B. CYTOPLASMIC MICROTUBULES. I. HYDRA. J Cell Biol. 1963 Aug;18:367–388. doi: 10.1083/jcb.18.2.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SOTELO J. R., TRUJILLO-CENOZ O. Electron microscope study of the kinetic apparatus in animal sperm cells. Z Zellforsch Mikrosk Anat. 1958;48(5):565–601. doi: 10.1007/BF00342732. [DOI] [PubMed] [Google Scholar]
  22. TELKKA A., FAWCETT D. W., CHRISTENSEN A. K. Further observations on the structure of the mammalian sperm tail. Anat Rec. 1961 Nov;141:231–245. doi: 10.1002/ar.1091410308. [DOI] [PubMed] [Google Scholar]
  23. WADA S. K., COLLIER J. R., DAN J. C. Studies on the acrosome. V. An egg-membrane lysin from the acrosomes of Mytilus edulis spermatozoa. Exp Cell Res. 1956 Feb;10(1):168–180. doi: 10.1016/0014-4827(56)90083-0. [DOI] [PubMed] [Google Scholar]
  24. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. YASUZUMI G., ISHIDA H. Spermatogenesis in animals as revealed by electron microscopy. II. Submicroscopic structure of developing spermatid nuclei of grasshopper. J Biophys Biochem Cytol. 1957 Sep 25;3(5):663–668. doi: 10.1083/jcb.3.5.663. [DOI] [PMC free article] [PubMed] [Google Scholar]

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