Skip to main content
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1965 Jan 1;24(1):157–161. doi: 10.1083/jcb.24.1.157

AN IMPROVED METHOD FOR THE ISOLATION OF RAT LIVER NUCLEI BY DENSITY CENTRIFUGATION

D M Gill 1
PMCID: PMC2106554  PMID: 14286292

Full Text

The Full Text of this article is available as a PDF (373.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHAUVEAU J., MOULE Y., ROUILLER C. Isolation of pure and unaltered liver nuclei morphology and biochemical composition. Exp Cell Res. 1956 Aug;11(2):317–321. doi: 10.1016/0014-4827(56)90107-0. [DOI] [PubMed] [Google Scholar]
  2. DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FISHER R. F., HOLBROOK D. J., Jr, IRVIN J. L. Density gradient isolation of rat liver nuclei with high DNA content. J Cell Biol. 1963 May;17:231–236. doi: 10.1083/jcb.17.2.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MILLER L. A., GOLDFEDER A. Adenylate kinase of nuclei and its distribution in several subcellular fractions of neoplastic tissue. Exp Cell Res. 1961 Mar;23:311–317. doi: 10.1016/0014-4827(61)90040-4. [DOI] [PubMed] [Google Scholar]
  6. PENNIALL R., HALL A. H., GRIFFIN J. B., IRVIN J. L. Formation of acid-labile phosphate by isolated mammalian nuclei. Arch Biochem Biophys. 1962 Jul;98:128–134. doi: 10.1016/0003-9861(62)90157-1. [DOI] [PubMed] [Google Scholar]
  7. RICE M. E., SHELTON E. Comparison of the reduction of two tetrazolium salts with succinoxidase activity of tissue homogenates. J Natl Cancer Inst. 1957 Jan;18(1):117–125. [PubMed] [Google Scholar]
  8. WILCZOK T., CHORAZY K. Isolation of liver nuclei. Nature. 1960 Nov 5;188:516–517. doi: 10.1038/188516b0. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES