Abstract
The deoxyribonuclease (DNase) activity of the dipteran (Chironomus thummi) salivary gland, measured both enzymatically and immunochemically, increases about 7-fold with the onset of metamorphosis. The increase in DNase activity occurs at a time when the activities of other enzymes and the total protein content are decreasing. The increased DNase activity is followed by glandular destruction. It is suggested that the alterations of this activity may be regulated by the activities of specific chromosomal sites, and that the enzyme may, at least in part, account for the glandular destruction observed at the time of increased enzyme activity.
Full Text
The Full Text of this article is available as a PDF (454.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CLEVER U. [Gene activity in the giant chromosomes of Chironomus tentans and its relation to development. I. Gene activation by ecdysone]. Chromosoma. 1961;12:607–675. doi: 10.1007/BF00328945. [DOI] [PubMed] [Google Scholar]
- KEYL H. G. Untersuchungen am Karyotypus von Chironomus thummi. I. Karte der Speicheldrüsen-Chromosomen von Chironomus thummi thummi und die cytologische Differenzierung der Subspezies Ch. th. thummi und Ch. th. piger. Chromosoma. 1957;8(6):739–756. [PubMed] [Google Scholar]
- LAUFER H., NAKASE Y. SALIVARY GLAND SECRETION AND ITS RELATION TO CHROMOSOMAL PUFFING IN THE DIPTERAN, CHIRONOMUS THUMMI. Proc Natl Acad Sci U S A. 1965 Mar;53:511–516. doi: 10.1073/pnas.53.3.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- PELLING G. Chromosomal synthesis of ribonucleic acid as shown by incorporation of uridine labelled with tritium. Nature. 1959 Aug 22;184(Suppl 9):655–656. doi: 10.1038/184655a0. [DOI] [PubMed] [Google Scholar]
- SIRLIN J. L., JACOB J., KATO K. I. The relation of messenger to nucleolar RNA. Exp Cell Res. 1962 Aug;27:355–359. doi: 10.1016/0014-4827(62)90245-8. [DOI] [PubMed] [Google Scholar]