Abstract
The formation of a soluble H3-thymidine derivative pool has been examined in Tetrahymena pyriformis as a function of macronuclear DNA synthesis during the cell life cycle. An autoradiographic technique which allows the detection of water-soluble materials within a cell has shown that these cells do not take up and retain exogenous H3-thymidine during G1 or G2. Uptake of H3-thymidine is restricted to the S period of the cell cycle. Additional autoradiographic experiments show, however, that a soluble pool of H3-thymidine derivatives persists from the end of one DNA synthesis period to the beginning of the next synthesis period in the subsequent cell cycle. Since this persisting pool cannot be labeled with H3-thymidine, the pool does not turn over during non-S periods.
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Selected References
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- BOLLUM F. J., POTTER V. R. Nucleic acid metabolism in regenerating rat liver. VI. Soluble enzymes which convert thymidine to thymidine phosphates and DNA. Cancer Res. 1959 Jun;19(5):561–565. [PubMed] [Google Scholar]
- BRANTON D., JACOBSON L. Dry, high resolution autoradiography. Stain Technol. 1962 Jul;37:239–242. doi: 10.3109/10520296209117744. [DOI] [PubMed] [Google Scholar]
- FEINENDEGEN L. E., BOND V. P. Differential uptake of 3Hthymidine into the soluble fraction of single bone marrow cells, determined by autoradiography. Exp Cell Res. 1962 Sep;27:474–484. doi: 10.1016/0014-4827(62)90013-7. [DOI] [PubMed] [Google Scholar]
- Hotta Y., Stern H. MOLECULAR FACETS OF MITOTIC REGULATION, I. SYNTHESIS OF THYMIDINE KINASE. Proc Natl Acad Sci U S A. 1963 May;49(5):648–654. doi: 10.1073/pnas.49.5.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STONE G. E., PRESCOTT D. M. CELL DIVISION AND DNA SYNTHESIS IN TETRAHYMENA PYRIFORMIS DEPRIVED OF ESSENTIAL AMINO ACIDS. J Cell Biol. 1964 May;21:275–281. doi: 10.1083/jcb.21.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEISSMAN S. M., SMELLIE R. M., PAUL J. Studies on the biosynthesis of deoxyribonucleic acid by extracts of mammalian cells. IV. The phosphorylation of thymidine. Biochim Biophys Acta. 1960 Dec 4;45:101–110. doi: 10.1016/0006-3002(60)91430-x. [DOI] [PubMed] [Google Scholar]