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. 1965 May 1;25(2):229–242. doi: 10.1083/jcb.25.2.229

SOME OBSERVATIONS ON THE PERMEABILITY OF MITOCHONDRIA TO SUCROSE

Henry Tedeschi 1
PMCID: PMC2106653  PMID: 14287176

Abstract

In experiments carried out with sucrose-isolated rat liver mitochondria, C14-sucrose seems to penetrate most of the mitochondrial space rapidly, whereas it seems to penetrate the remainder relatively slowly. However, taking into account the wide size distribution found in isolated preparations, these rates have been found consistent with the kinetics expected from the assumption of a single compartment per mitochondrion. These data cannot be readily interpreted on the basis of two mitochondrial spaces, one highly permeable, and another relatively impermeable to sucrose.

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Selected References

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  1. AMOORE J. E., BARTLEY W. The permeability of isolated rat-liver mitochondria to sucrose, sodium chloride and potassium chloride at 0 degrees. Biochem J. 1958 Jun;69(2):223–236. doi: 10.1042/bj0690223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BARTLEY W. Solute movemetns during volume changes in rat-liver mitochondria. Biochem J. 1961 Jul;80:46–57. doi: 10.1042/bj0800046. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BRIERLEY G., MURER E., BACHMANN E., GREEN D. E. STUDIES ON ION TRANSPORT. II. THE ACCUMULATION OF INORGANIC PHOSPHATE AND MAGNESIUM IONS BY HEART MITOCHONDRIA. J Biol Chem. 1963 Oct;238:3482–3489. [PubMed] [Google Scholar]
  4. CHANCE B., PARSONS D. F., WILLIAMS G. R. CYTOCHROME CONTENT OF MITOCHONDRIA STRIPPED OF INNER MEMBRANE STRUCTURE. Science. 1964 Jan 10;143(3602):136–139. doi: 10.1126/science.143.3602.136. [DOI] [PubMed] [Google Scholar]
  5. DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JACKSON K. L., PACE N. Some permeability properties of isolated rat liver cell mitochondria. J Gen Physiol. 1956 Sep 20;40(1):47–71. doi: 10.1085/jgp.40.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. JACKSON K. L., WALKER E. L., PACE N. Centrifugal preparation of rat liver mitochondria free of microsomes. Science. 1953 Jul 31;118(3057):136–137. doi: 10.1126/science.118.3057.136. [DOI] [PubMed] [Google Scholar]
  8. LAIRD A. K., NYGAARD O., RIS H., BARTON A. D. Separation of mitochondria into two morphologically and biochemically distinct types. Exp Cell Res. 1953 Sep;5(1):147–160. doi: 10.1016/0014-4827(53)90100-1. [DOI] [PubMed] [Google Scholar]
  9. MALAMED S., RECKNAGEL R. O. The osmotic behavior of the sucrose-inaccessible space of mitochondrial pellets from rat liver. J Biol Chem. 1959 Nov;234:3027–3030. [PubMed] [Google Scholar]
  10. PAULY H., PACKER L., SCHWAN H. P. Electrical properties of mitochondrial membranes. J Biophys Biochem Cytol. 1960 Jul;7:589–601. doi: 10.1083/jcb.7.4.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PAULY H., PACKER L. The relationship of internal conductance and membrane capacity to mitochondrial volume. J Biophys Biochem Cytol. 1960 Jul;7:603–612. doi: 10.1083/jcb.7.4.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SHARE L. Depletion and reaccumulation of mitochondrial sodium and potassium: effect of adrenalectomy. Am J Physiol. 1958 Jul;194(1):47–52. doi: 10.1152/ajplegacy.1958.194.1.47. [DOI] [PubMed] [Google Scholar]
  13. SHARE L., HANSROTE R. W. Permeability of rat liver microsomes to sucrose and carboxypolyglucose in vitro. J Biophys Biochem Cytol. 1960 Apr;7:239–242. doi: 10.1083/jcb.7.2.239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SHARE L. Volumes of distribution of hemoglobin, of [14C] carboxypolyglucose and of [14C]sucrose in pellets of rat-liver mitochondria. Biochim Biophys Acta. 1960 Feb 12;38:154–155. doi: 10.1016/0006-3002(60)91209-9. [DOI] [PubMed] [Google Scholar]
  15. TEDESCHI H., HARRIS D. L. Some observations on the photometric estimation of mitochondrial volume. Biochim Biophys Acta. 1958 May;28(2):392–402. doi: 10.1016/0006-3002(58)90487-6. [DOI] [PubMed] [Google Scholar]
  16. TEDESCHI H., HARRIS D. L. The osmotic behavior and permeability to non-electrolytes of mitochondria. Arch Biochem Biophys. 1955 Sep;58(1):52–67. doi: 10.1016/0003-9861(55)90092-8. [DOI] [PubMed] [Google Scholar]
  17. TEDESCHI H. The structure of the mitochondrial membrane: inferences from permeability properties. J Biophys Biochem Cytol. 1959 Oct;6:241–252. doi: 10.1083/jcb.6.2.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. WERKHEISER W. C., BARTLEY W. The study of steady-state concentrations of internal solutes of mitochondria by rapid centrifugal transfer to a fixation medium. Biochem J. 1957 May;66(1):79–91. doi: 10.1042/bj0660079. [DOI] [PMC free article] [PubMed] [Google Scholar]

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