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. 1988 Jan;170(1):468–470. doi: 10.1128/jb.170.1.468-470.1988

Rapid identification of bacterial genes that are lethal when cloned on multicopy plasmids.

C M Berg 1, L Liu 1, B Wang 1, M D Wang 1
PMCID: PMC210670  PMID: 3275630

Abstract

A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn10, which is lethal when in multiple copies in the presence of 25 micrograms of tetracycline per ml.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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