Abstract
Actidione, reportedly a specific inhibitor of protein synthesis, was found to reduce the incorporation of labeled amino acids into proteins of the slime mold Physarum polycephalum without drastically inhibiting the incorporation of nucleic acid precursors into RNA. This inhibitor was found to completely block the ensuing mitosis if it was added at any time between telophase and late prophase. Plasmodia given Actidione in late prophase (about the time of nucleolar dissolution) went on through telophase to reconstruction even though nuclear amino acid incorporation was drastically reduced during that period.
Full Text
The Full Text of this article is available as a PDF (361.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BENNETT L. L., Jr, SMITHERS D., WARD C. T. INHIBITION OF DNA SYNTHESIS IN MAMMALIAN CELLS BY ACTIDIONE. Biochim Biophys Acta. 1964 May 18;87:60–69. doi: 10.1016/0926-6550(64)90047-7. [DOI] [PubMed] [Google Scholar]
- EDSTROM J. E., GRAMPP W., SCHOR N. The intracellular distribution and heterogeneity of ribonucleic acid in starfish oocytes. J Biophys Biochem Cytol. 1961 Dec;11:549–557. doi: 10.1083/jcb.11.3.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ELSDALE T. R., FISCHBERG M., SMITH S. A mutation that reduces nucleolar number in Xenopus laevis. Exp Cell Res. 1958 Jun;14(3):642–643. doi: 10.1016/0014-4827(58)90175-7. [DOI] [PubMed] [Google Scholar]
- GORNALL A. G., BARDAWILL C. J., DAVID M. M. Determination of serum proteins by means of the biuret reaction. J Biol Chem. 1949 Feb;177(2):751–766. [PubMed] [Google Scholar]
- GROSS P. R., MALKIN L. I., MOYER W. A. TEMPLATES FOR THE FIRST PROTEINS OF EMBRYONIC DEVELOPMENT. Proc Natl Acad Sci U S A. 1964 Mar;51:407–414. doi: 10.1073/pnas.51.3.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GUTTES E., GUTTES S., RUSCH H. P. Morphological observations on growth and differentation of Physarum polycephalum grown in pure culture. Dev Biol. 1961 Oct;3:588–614. doi: 10.1016/0012-1606(61)90034-3. [DOI] [PubMed] [Google Scholar]
- Gaulden M. E., Perry R. P. INFLUENCE OF THE NUCLEOLUS ON MITOSIS AS REVEALED BY ULTRAVIOLET MICROBEAM IRRADIATION. Proc Natl Acad Sci U S A. 1958 Jun;44(6):553–559. doi: 10.1073/pnas.44.6.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- MITTERMAYER C., BRAUN R., RUSCH H. P. THE EFFECT OF ACTINOMYCIN D ON THE TIMING OF MITOSIS IN PHYSARUM POLYCEPHALUM. Exp Cell Res. 1965 Apr;38:33–41. doi: 10.1016/0014-4827(65)90424-6. [DOI] [PubMed] [Google Scholar]
- McLEISH J. Radiation sensitivity and the mitotic cycle in Vicia faba. Nature. 1955 May 21;175(4464):890–891. doi: 10.1038/175890a0. [DOI] [PubMed] [Google Scholar]
- SIRLIN J. L. THE INTRACELLULAR TRANSFER OF GENETIC INFORMATION. Int Rev Cytol. 1963;15:35–96. doi: 10.1016/s0074-7696(08)61115-8. [DOI] [PubMed] [Google Scholar]
- VON BORSTEL R. C., REKEMEYER M. L. Division of a nucleus lacking a nucleolus. Nature. 1958 Jun 7;181(4623):1597–1598. doi: 10.1038/1811597a0. [DOI] [PubMed] [Google Scholar]