Abstract
The effects of whole body x-irradiation on the fine structure of sympathetic neurons were studied in 15 unanesthetized adult frogs (Rana pipiens), as seen at intervals ranging from 1 hour to 2 weeks after single exposures to 1000 r and 2000 r. Using standard procedures, the lumbar sympathetic ganglia of experimental and 20 control animals were prepared for electron microscope examination. Radiation produced conspicuous but irregular and variable deterioration, swelling, and clearing of neuronal lysosomes. These changes may have been due to an increased permeability of lysosomal membranes, causing the entry of fluid into lysosomes and their swelling and deterioration, but a pronounced escape of lysosomal enzymes into the cytoplasm was questionable. Less frequent were the dilatation and the parallel layering or complete fusion and tight packing of the rough-edged endoplasmic reticulum. The number of vacuoles, probably derived from Golgi cisternae, was somewhat increased. These vacuoles were conjectured to serve the "sequestration" of damaged cytoplasmic areas. Abnormal amounts of presumptive glycogen granules occupied some axons of myelinated and unmyelinated fibers, especially of presynaptic nerve fibers. This was assumed to be due to a decreased breakdown of glycogen and probably caused the interruption of the transmission of nerve impulses in presynaptic fibers. The maximal incidence of these alterations seemingly occurred 8 days after exposure to 1000 r, and 1 hour after x-irradiation with 2000 r. Signs of recovery appeared 2 weeks after exposure to 2000 r.
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- ANDRES K. H. ELEKTRONENMIKROSKOPISCHE UNTERSUCHUNGEN UEBER STRUKTURVERAENDERUNGEN AN BLUTGEFAESSEN UND AM ENDONEURIUM IN SPINALGANGLIEN VON RATTEN NACH BESTRAHLUNG MIT 185 MEV-PROTONEN. Z Zellforsch Mikrosk Anat. 1963 Oct 8;61:23–51. [PubMed] [Google Scholar]
- ANDRES K. H. ELEKTRONENMIKROSKOPISCHE UNTERSUCHUNGEN UEBER STRUKTURVERAENDERUNGEN AN DEN NERVENFASERN IN RATTENSPINALGANGLIEN NACH BESTRAHLUNG MIT 185 MEV-PROTONEN. Z Zellforsch Mikrosk Anat. 1963 Oct 8;61:1–22. [PubMed] [Google Scholar]
- BRINKMAN R., LAMBERTS H. B. Direct registration of an instantaneous x-ray effect in rats and man. Nature. 1958 Mar 15;181(4611):774–775. doi: 10.1038/181774a0. [DOI] [PubMed] [Google Scholar]
- DE DUVE C., PRESSMAN B. C., GIANETTO R., WATTIAUX R., APPELMANS F. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue. Biochem J. 1955 Aug;60(4):604–617. doi: 10.1042/bj0600604. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ESSNER E., NOVIKOFF A. B. Localization of acid phosphatase activity in hepatic lysosomes by means of electron microscopy. J Biophys Biochem Cytol. 1961 Apr;9:773–784. doi: 10.1083/jcb.9.4.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOLT S. J., HICKS R. M. The localization of acid phosphatase in rat liver cells as revealed by combined cytochemical staining and electron microscopy. J Biophys Biochem Cytol. 1961 Oct;11:47–66. doi: 10.1083/jcb.11.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KAGAN E. H., BROWNSON R. H., SUTER D. B. Radiation-caused cytochemical changes in neurons. Arch Pathol. 1962 Sep;74:195–203. [PubMed] [Google Scholar]
- NOVIKOFF A. B., BEAUFAY H., DE DUVE C. Electron microscopy of lysosomerich fractions from rat liver. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):179–184. [PMC free article] [PubMed] [Google Scholar]
- NOVIKOFF A. B., ESSNER E. Pathological changes in cytoplasmic organelles. Fed Proc. 1962 Nov-Dec;21:1130–1142. [PubMed] [Google Scholar]
- PICK J. On the submicroscopic organization of the myelinated sympathetic nerve fiber in the frog (Rana pipiens). Anat Rec. 1962 Dec;144:295–325. doi: 10.1002/ar.1091440404. [DOI] [PubMed] [Google Scholar]
- PICK J. Sympathectomy in amphibians; anatomical considerations. J Comp Neurol. 1957 Apr;107(2):169–207. doi: 10.1002/cne.901070202. [DOI] [PubMed] [Google Scholar]
- PICK J. The submicroscopic organization of the sympathetic ganglion in the frog (Rana pipiens). J Comp Neurol. 1963 Jun;120:409–462. doi: 10.1002/cne.901200304. [DOI] [PubMed] [Google Scholar]
- PITCOCK J. A. An electron microscopic study of acute radiation injury of the rat brain. Lab Invest. 1962 Jan;2:32–44. [PubMed] [Google Scholar]
- REVEL J. P. ELECTRON MICROSCOPY OF GLYCOGEN. J Histochem Cytochem. 1964 Feb;12:104–114. doi: 10.1177/12.2.104. [DOI] [PubMed] [Google Scholar]
- RITCHIE J. M., STRAUB R. W. The hyperpolarization which follows activity in mammalian non-medullated fibres. J Physiol. 1957 Apr 3;136(1):80–97. doi: 10.1113/jphysiol.1957.sp005744. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SWIFT H., HRUBAN Z. FOCAL DEGRADATION AS A BIOLOGICAL PROCESS. Fed Proc. 1964 Sep-Oct;23:1026–1037. [PubMed] [Google Scholar]
- TRUMP B. F., JANIGAN D. T. The pathogenesis of cytologic vacuolization in sucrose nephrosis. An electron microscopic and histochemical study. Lab Invest. 1962 May;11:395–411. [PubMed] [Google Scholar]
- VAN LANCKER J. L., HOLTZER R. L. The release of acid phosphatase and beta-glucuronidase from cytoplasmic granules in the early course of autolysis. Am J Pathol. 1959 May-Jun;35(3):563–573. [PMC free article] [PubMed] [Google Scholar]