Abstract
In a number of mammalian cell strains nucleoli persisted through mitosis. This phenomenon was especially pronounced in several cell lines derived from Chinese hamster tissues. All the methods employed, including radioautography with tritiated uridine, cytochemical stains (methyl green-pyronin and azure B), fluorescent microscopy (coriphosphine O), ribonuclease digestion, and electron microscopy, demonstrated that the bodies identified as persistent nucleoli in the mitotic stages had the same characteristics as did the nucleoli in the interphase. Persistent nucleoli may attach to the chromosomes or may be free in the cytoplasm. In cells where no persistent nucleoli as such were noted, nucleolar material was observed to attach to the chromosomes in shapeless masses which moved with the chromosomes during anaphase. At least a portion of the nucleolar material was included in the daughter nuclei, presumably for immediate use for protein synthesis after cell division.
Full Text
The Full Text of this article is available as a PDF (1.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CONGER A. D., FAIRCHILD L. M. A quick-freeze method for making smear slides permanent. Stain Technol. 1953 Nov;28(6):281–283. doi: 10.3109/10520295309105555. [DOI] [PubMed] [Google Scholar]
- HEATH J. C. The effect of cobalt on mitosis in tissue culture. Exp Cell Res. 1954 May;6(2):311–320. doi: 10.1016/0014-4827(54)90178-0. [DOI] [PubMed] [Google Scholar]
- HSU T. C., HUMPHREY R. M., SOMERS C. E. PERSISTENT NUCLEOLI IN ANIMAL CELLS FOLLOWING TREATMENTS WITH FLUORODEOXYURIDINE AND THYMIDINE. Exp Cell Res. 1964 Jan;33:74–77. doi: 10.1016/s0014-4827(64)81014-4. [DOI] [PubMed] [Google Scholar]
- HSU T. C. MAMMALIAN CHROMOSOMES IN VITRO. 18. DNA REPLICATION IN THE CHINESE HAMSTER. J Cell Biol. 1964 Oct;23:53–62. doi: 10.1083/jcb.23.1.53. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HSU T. C., MERCHANT D. J. Mammalian chromosomes in vitro. XIV. Genotypic replacement in cell populations. J Natl Cancer Inst. 1961 May;26:1075–1083. [PubMed] [Google Scholar]
- HSU T. C., SOMERS C. E. Effect of 5-bromodeoxyuridine on mamalian chromosomes. Proc Natl Acad Sci U S A. 1961 Mar 15;47:396–403. doi: 10.1073/pnas.47.3.396. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KURNICK N. B. Pyronin Y in the methyl-green-pyronin histological stain. Stain Technol. 1955 Sep;30(5):213–230. doi: 10.3109/10520295509114469. [DOI] [PubMed] [Google Scholar]
- LAFONTAINE J. G., CHOUINARD L. A. A correlated light and electron microscope study of the nucleolar material during mitosis in Vicia faba. J Cell Biol. 1963 Apr;17:167–201. doi: 10.1083/jcb.17.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOVE R., SUSKIND R. G. Further observations on the ribonucleoproteins of mitotically dividing mammalian cells. Exp Cell Res. 1961 Jan;22:193–207. doi: 10.1016/0014-4827(61)90098-2. [DOI] [PubMed] [Google Scholar]
- MOORHEAD P. S., NOWELL P. C., MELLMAN W. J., BATTIPS D. M., HUNGERFORD D. A. Chromosome preparations of leukocytes cultured from human peripheral blood. Exp Cell Res. 1960 Sep;20:613–616. doi: 10.1016/0014-4827(60)90138-5. [DOI] [PubMed] [Google Scholar]