Skip to main content
PMC home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1965 Dec 1;27(3):451–457. doi: 10.1083/jcb.27.3.451

NUCLEAR DNA AND CYTOPLASMIC DNA FROM TISSUES OF HIGHER PLANTS

Yasuo Hotta 1, Alix Bassel 1, Herbert Stern 1
PMCID: PMC2106767  PMID: 5885425

Abstract

Young wheat roots were labeled with 32P-inorganic phosphate. Following the labeling period, roots were homogenized in a sucrose medium and fractionated into nuclei, cytoplasmic particles (including proplastids and mitochondria), and a soluble fraction containing most of the microsomes. DNA prepared from the particles had a higher buoyant density than that from the nuclei and showed a marked loss in total label if the roots were exposed to non-radioactive medium for 48 hours prior to fractionation of the cells.

Full Text

The Full Text of this article is available as a PDF (439.8 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHUN E. H., VAUGHAN M. H., Jr, RICH A. THE ISOLATION AND CHARACTERIZATION OF DNA ASSOCIATED WITH CHLOROPLAST PREPARATIONS. J Mol Biol. 1963 Aug;7:130–141. doi: 10.1016/s0022-2836(63)80042-x. [DOI] [PubMed] [Google Scholar]
  2. GIBOR A., IZAWA M. THE DNA CONTENT OF THE CHLOROPLASTS OF ACETABULARIA. Proc Natl Acad Sci U S A. 1963 Dec;50:1164–1169. doi: 10.1073/pnas.50.6.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HOTTA Y., BASSEL A. MOLECULAR SIZE AND CIRCULARITY OF DNA IN CELLS OF MAMMALS AND HIGHER PLANTS. Proc Natl Acad Sci U S A. 1965 Feb;53:356–362. doi: 10.1073/pnas.53.2.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Johnston F. B., Nasatir M., Stern H. The Isolation of Nuclei and Oxidative Particles from Viable Wheat Embryos. Plant Physiol. 1957 Mar;32(2):124–129. doi: 10.1104/pp.32.2.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LUCK D. J. Formation of mitochondria in Neurospora crassa. A quantitative radioautographic study. J Cell Biol. 1963 Mar;16:483–499. doi: 10.1083/jcb.16.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Meselson M., Stahl F. W., Vinograd J. EQUILIBRIUM SEDIMENTATION OF MACROMOLECULES IN DENSITY GRADIENTS. Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):581–588. doi: 10.1073/pnas.43.7.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. NASS M. M., NASS S. INTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS. I. FIXATION AND ELECTRON STAINING REACTIONS. J Cell Biol. 1963 Dec;19:593–611. doi: 10.1083/jcb.19.3.593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PFAU C. J., McCREA J. F. Release of deoxyribonucleic acid from vaccinia virus by 2-mercaptoethanol and pronase. Nature. 1962 Jun 2;194:894–895. doi: 10.1038/194894a0. [DOI] [PubMed] [Google Scholar]
  9. RIS H., PLAUT W. Ultrastructure of DNA-containing areas in the chloroplast of Chlamydomonas. J Cell Biol. 1962 Jun;13:383–391. doi: 10.1083/jcb.13.3.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SAMPSON M., KATOH A., HOTTA Y., STERN H. METABOLICALLY LABILE DEOXYRIBONUCLEIC ACID. Proc Natl Acad Sci U S A. 1963 Sep;50:459–463. doi: 10.1073/pnas.50.3.459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. STONE G. E., PRESCOTT D. M. CELL DIVISION AND DNA SYNTHESIS IN TETRAHYMENA PYRIFORMIS DEPRIVED OF ESSENTIAL AMINO ACIDS. J Cell Biol. 1964 May;21:275–281. doi: 10.1083/jcb.21.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SUEOKA N., CHENG T. Y. Fractionation of nucleic acids with the methylated albumin column. J Mol Biol. 1962 Mar;4:161–172. doi: 10.1016/s0022-2836(62)80048-5. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES