Abstract
The deformability of the surface membranes of Sarcoma 37 and Ehrlich murine ascites tumor cells was assessed by the pressure required to suck a hemispherical bulge from these cells into a micropipette. It was shown that treatment with neuraminidase allowed the cells to be deformed with significantly less suction, and that enzymatic treatment also produced a significant reduction in surface charge as determined by measurement of cellular electrophoretic mobility. It is suggested that the increase in cellular deformability may be related to charge reduction, and that the charge at the cell periphery may affect not only the magnitude of the potential energy barriers hindering contact between cells, but also the ease with which cells can form low radius of curvature probes in order to help overcome these barriers.
Full Text
The Full Text of this article is available as a PDF (293.3 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BANGHAM A. D., HEARD D. H., FLEMANS R., SEAMAN G. V. An apparatus for microelectrophoresis of small particles. Nature. 1958 Sep 6;182(4636):642–644. doi: 10.1038/182642a0. [DOI] [PubMed] [Google Scholar]
- COOK G. M., HEARD D. H., SEAMAN G. V. Sialic acids and the electrokinetic charge of the human erythrocyte. Nature. 1961 Jul 1;191:44–47. doi: 10.1038/191044a0. [DOI] [PubMed] [Google Scholar]
- CURTIS A. S. Cell contact and adhesion. Biol Rev Camb Philos Soc. 1962 Feb;37:82–129. doi: 10.1111/j.1469-185x.1962.tb01605.x. [DOI] [PubMed] [Google Scholar]
- GOTTSCHALK A. Correlation between composition, structure, shape and function of a salivary mucoprotein. Nature. 1960 Jun 18;186:949–951. doi: 10.1038/186949a0. [DOI] [PubMed] [Google Scholar]
- HAKOMORI S. I., JEANLOZ R. W. Isolation and characterization of glycolipids from erythrocytes of human blood A (plus) and B (plus). J Biol Chem. 1961 Nov;236:2827–2834. [PubMed] [Google Scholar]
- LESSEPS R. J. CELL SURFACE PROJECTIONS: THEIR ROLE IN THE AGGREGATION OF EMBRYONIC CHICK CELLS AS REVEALED BY ELECTRON MICROSCOPY. J Exp Zool. 1963 Jul;153:171–182. doi: 10.1002/jez.1401530209. [DOI] [PubMed] [Google Scholar]
- MOSCONA A. A. Cellular interactions in experimental histogenesis. Int Rev Exp Pathol. 1962;1:371–428. [PubMed] [Google Scholar]
- RAND R. P., BURTON A. C. MECHANICAL PROPERTIES OF THE RED CELL MEMBRANE. I. MEMBRANE STIFFNESS AND INTRACELLULAR PRESSURE. Biophys J. 1964 Mar;4:115–135. doi: 10.1016/s0006-3495(64)86773-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROTH F. B., PILLEMER L. The separation of alpha toxin (lecithinase) from filtrates of Clostridium welchii. J Immunol. 1953 Jun;70(6):533–537. [PubMed] [Google Scholar]
- SIMON-REUSS I., COOK G. M., SEAMAN G. V., HEARD D. H. ELECTROPHORETIC STUDIES ON SOME TYPES OF MAMMALIAN TISSUE CELL. Cancer Res. 1964 Dec;24:2038–2043. [PubMed] [Google Scholar]
- Steinberg M. S. ON THE MECHANISM OF TISSUE RECONSTRUCTION BY DISSOCIATED CELLS, III. FREE ENERGY RELATIONS AND THE REORGANIZATION OF FUSED, HETERONOMIC TISSUE FRAGMENTS. Proc Natl Acad Sci U S A. 1962 Oct;48(10):1769–1776. doi: 10.1073/pnas.48.10.1769. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TAYLOR A. C., ROBBINS E. Observations on microextensions from the surface of isolated vertebrate cells. Dev Biol. 1963 Mar;6:660–673. doi: 10.1016/0012-1606(63)90150-7. [DOI] [PubMed] [Google Scholar]
- TIETZ N. W., BORDEN T., STEPLETON J. D. An improved method for the determination of lipase in serum. Am J Clin Pathol. 1959 Feb;31(2):148–154. doi: 10.1093/ajcp/31.2.148. [DOI] [PubMed] [Google Scholar]
- UHLENBRUCK G. Action of proteolytic enzymes on the human erythrocyte surface. Nature. 1961 Apr 8;190:181–181. doi: 10.1038/190181a0. [DOI] [PubMed] [Google Scholar]
- WEISS L. Sialic acid as a structural component of some mammalian tissue cell surfaces. Nature. 1961 Sep 9;191:1108–1109. doi: 10.1038/1911108b0. [DOI] [PubMed] [Google Scholar]