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. 1966 Mar 1;28(3):413–421. doi: 10.1083/jcb.28.3.413

STUDIES ON INSULIN BIOSYNTHESIS

Subcellular Distribution of Leucine-H3 Radioactivity During Incubation of Goosefish Islet Tissue

G Eric Bauer 1, Arnold W Lindall Jr 1, Padmaker K Dixit 1, Gordon Lester 1, Arnold Lazarow 1
PMCID: PMC2106944  PMID: 5335636

Abstract

Islet tissue from the goosefish (Lophius piscatorius) was incubated in the presence of leucine-H3. The tissue was then separated into subcellular fractions and the radioactivity determined in total acid alcohol-soluble proteins, insoluble proteins, and insulin. At any time, microsomal protein had a higher activity than secretion granule protein. Pulse-chase experiments further suggest the microsomes as primary sites of protein synthesis. The data are evidence for microsomal synthesis of insulin and for its subsequent transfer into the secretion granules.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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