Abstract
Many laboratory strains of Bacillus subtilis contain 9 rather than 10 rRNA operons due to deletions occurring within the rrnJ-rrnW or rrnI-rrnH-rrnG gene cluster. These operons are members of two sets of closely spaced clusters located in the cysA-aroI region. Analysis of rescued DNA from integrants with insertions into rrnG and rrnH indicated that these tandemly arranged operons allowed frequent deletions of an rrn operon equivalent. These events may arise spontaneously by intrachromosomal recombination or by simultaneous double crossovers with a multimeric integrative plasmid.
Full text
PDF





Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chow L. T., Davidson N. Electron microscope mapping of the distribution of ribosomal genes of the Bacillus subtilis chromosome. J Mol Biol. 1973 Apr 5;75(2):265–279. doi: 10.1016/0022-2836(73)90020-x. [DOI] [PubMed] [Google Scholar]
- Dedonder R. A., Lepesant J. A., Lepesant-Kejzlarová J., Billault A., Steinmetz M., Kunst F. Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168. Appl Environ Microbiol. 1977 Apr;33(4):989–993. doi: 10.1128/aem.33.4.989-993.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dubnau D., Goldthwaite C., Smith I., Marmur J. Genetic mapping in Bacillus subtilis. J Mol Biol. 1967 Jul 14;27(1):163–185. doi: 10.1016/0022-2836(67)90358-0. [DOI] [PubMed] [Google Scholar]
- Ellwood M., Nomura M. Deletion of a ribosomal ribonucleic acid operon in Escherichia coli. J Bacteriol. 1980 Aug;143(2):1077–1080. doi: 10.1128/jb.143.2.1077-1080.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gottlieb P., LaFauci G., Rudner R. Alterations in the number of rRNA operons within the Bacillus subtilis genome. Gene. 1985;33(3):259–268. doi: 10.1016/0378-1119(85)90233-1. [DOI] [PubMed] [Google Scholar]
- Hill C. W., Harnish B. W. Transposition of a chromosomal segment bounded by redundant rRNA genes into other rRNA genes in Escherichia coli. J Bacteriol. 1982 Feb;149(2):449–457. doi: 10.1128/jb.149.2.449-457.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LaFauci G., Widom R. L., Eisner R. L., Jarvis E. D., Rudner R. Mapping of rRNA genes with integrable plasmids in Bacillus subtilis. J Bacteriol. 1986 Jan;165(1):204–214. doi: 10.1128/jb.165.1.204-214.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lehner A. F., Harvey S., Hill C. W. Mapping and spacer identification of rRNA operons of Salmonella typhimurium. J Bacteriol. 1984 Nov;160(2):682–686. doi: 10.1128/jb.160.2.682-686.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loughney K., Lund E., Dahlberg J. E. Deletion of an rRNA gene set in Bacillus subtilis. J Bacteriol. 1983 Apr;154(1):529–532. doi: 10.1128/jb.154.1.529-532.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vold B. S. Structure and organization of genes for transfer ribonucleic acid in Bacillus subtilis. Microbiol Rev. 1985 Mar;49(1):71–80. doi: 10.1128/mr.49.1.71-80.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ward J. B., Jr, Zahler S. A. Genetic studies of leucine biosynthesis in Bacillus subtilis. J Bacteriol. 1973 Nov;116(2):719–726. doi: 10.1128/jb.116.2.719-726.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wawrousek E. F., Hansen J. N. Structure and organization of a cluster of sic tRNA genes in the space between tandem ribosomal RNA gene sets in Bacillus subtilis. J Biol Chem. 1983 Jan 10;258(1):291–298. [PubMed] [Google Scholar]