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. 1966 Aug 1;30(2):424–432. doi: 10.1083/jcb.30.2.424

A NEW STAINING METHOD (OTO) FOR ENHANCING CONTRAST OF LIPID-CONTAINING MEMBRANES AND DROPLETS IN OSMIUM TETROXIDE-FIXED TISSUE WITH OSMIOPHILIC THIOCARBOHYDRAZIDE (TCH)

Arnold M Seligman 1, Hannah L Wasserkrug 1, Jacob S Hanker 1
PMCID: PMC2106998  PMID: 4165523

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DALTON A. J., ZEIGEL R. F. A simplified method of staining thin sections of biolgical material with lead hydroxide for electron microscopy. J Biophys Biochem Cytol. 1960 Apr;7:409–410. doi: 10.1083/jcb.7.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HAKE T. STUDIES ON THE REACTIONS OF OSO4 AND KMNO4 WITH AMINO ACIDS, PEPTIDES, AND PROTEINS. Lab Invest. 1965 Jun;14:1208–1212. [PubMed] [Google Scholar]
  3. HANKER J. S., SEAMAN A. R., WEISS L. P., UENO H., BERGMAN R. A., SELIGMAN A. M. OSMIOPHILIC REAGENTS: NEW CYTOCHEMICAL PRINCIPLE FOR LIGHT AND ELECTRON MICROSCOPY. Science. 1964 Nov 20;146(3647):1039–1043. doi: 10.1126/science.146.3647.1039. [DOI] [PubMed] [Google Scholar]
  4. Hanker J. S., Deb C., Wasserkrug H. L., Seligman A. M. Staining tissue for light and electron microscopy by bridging metals with multidentate ligands. Science. 1966 Jun 17;152(3729):1631–1634. doi: 10.1126/science.152.3729.1631. [DOI] [PubMed] [Google Scholar]
  5. MOUDRIANAKIS E. N., BEER M. BASE SEQUENCE DETERMINATION IN NUCLEIC ACIDS WITH THE ELECTRON MICROSCOPE. 3. CHEMISTRY AND MICROSCOPY OF GUANINE-LABELED DNA. Proc Natl Acad Sci U S A. 1965 Mar;53:564–571. doi: 10.1073/pnas.53.3.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. PARSONS D. F. A simple method for obtaining increased contrast in araldite sections by using postfixation staining of tissues with potassium permanganate. J Biophys Biochem Cytol. 1961 Nov;11:492–497. doi: 10.1083/jcb.11.2.492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. STOECKENIUS W., MAHR S. C. STUDIES ON THE REACTION OF OSMIUM TETROXIDE WITH LIPIDS AND RELATED COMPOUNDS. Lab Invest. 1965 Jun;14:1196–1207. [PubMed] [Google Scholar]
  9. STOECKENIUS W. Structure of the plasma membrane. An electron-microscope study. Circulation. 1962 Nov;26:1066–1069. doi: 10.1161/01.cir.26.5.1066. [DOI] [PubMed] [Google Scholar]
  10. Seligman A. M., Hanker J. S., Wasserkrug H., Dmochowski H., Katzoff L. Histochemical demonstration of some oxidized macromolecules with thiocarbohydrazide (tch) or thiosemicarbazide (TSC) and osmium tetroxide. J Histochem Cytochem. 1965 Nov-Dec;13(8):629–639. doi: 10.1177/13.8.629. [DOI] [PubMed] [Google Scholar]
  11. Seligman A. M., Ueno H., Wasserkrug H. L., Hanker J. S. Esterase method for light and electron microscopy via the formation of osmiophilic diazothioethers (1, 2). Ann Histochim. 1966 Apr-Jun;11(2):115–129. [PubMed] [Google Scholar]
  12. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium. J Biophys Biochem Cytol. 1958 Nov 25;4(6):727–730. doi: 10.1083/jcb.4.6.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Zobel C. R., Beer M. The use of heavy metal salts as electron stains. Int Rev Cytol. 1965;18:363–400. doi: 10.1016/s0074-7696(08)60558-6. [DOI] [PubMed] [Google Scholar]

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