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. 1966 Sep 1;30(3):465–475. doi: 10.1083/jcb.30.3.465

THE ACTION OF EXCESS OF VITAMIN A ALCOHOL ON THE FINE STRUCTURE OF RAT DERMAL FIBROBLASTS

Mary R Daniel 1, J T Dingle 1, Audrey M Glauert 1, J A Lucy 1
PMCID: PMC2107025  PMID: 5971002

Abstract

Rat dermal fibroblasts were grown as monolayers, and changes in the fine structure of the cells that occurred during 12 hr incubation in a medium containing protein and excess of retinol (vitamin A alcohol) were studied by electron microscopy. There is little change during the first 6 hr, although some of the nuclei have highly convoluted membranes. During the subsequent 3 hr, there is some disorganization of the mitochondrial cristae; the cisternae of the rough-surfaced endoplasmic reticulum diminish in number; and the amount of smooth membranous material and free ribosomes increases. There is a rapid decline in the respiratory activity of the cells after 6 hr exposure to the vitamin. It is concluded that the primary action of excess of retinol is to cause alterations in the membranes of the cells and that these alterations affect the functions of the mitochondria and endoplasmic reticulum.

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Selected References

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  1. BARRNETT R. J., BALL E. G. Metabolic and ultrastructural changes induced in adipose tissue by insulin. J Biophys Biochem Cytol. 1960 Sep;8:83–101. doi: 10.1083/jcb.8.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bangham A. D., Dingle J. T., Lucy J. A. Studies on the mode of action of excess of vitamin A. 9. Penetration of lipid monolayers by compounds in the vitamin A series. Biochem J. 1964 Jan;90(1):133–140. doi: 10.1042/bj0900133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHAPMAN J. A. Fibroblasts and collagen. Br Med Bull. 1962 Sep;18:233–237. doi: 10.1093/oxfordjournals.bmb.a069985. [DOI] [PubMed] [Google Scholar]
  4. Campbell P. N., Cooper C., Hicks M. Studies on the role of the morphological constituents of the microsome fraction from rat liver in protein synthesis. Biochem J. 1964 Aug;92(2):225–234. doi: 10.1042/bj0920225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DANIEL M., DINGLE J. T., WEEB M., HEATH J. C. The biological action of cobalt and other metals. I. The effect of cobalt on the morphology and metabolism of rat fibroblasts in vitro. Br J Exp Pathol. 1963 Apr;44:163–176. [PMC free article] [PubMed] [Google Scholar]
  6. DINGLE J. T., LUCY J. A., FELL H. B. Studies on the mode of action of excess of vitamin A. 1. Effect of excess of vitamin A on the metabolism and composition of embryonic chick-limb cartilage grown in organ culture. Biochem J. 1961 Jun;79:497–500. doi: 10.1042/bj0790497. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DINGLE J. T., LUCY J. A. Studies on the mode of action of excess of vitamin A. 5. The effect of vitamin A on the stability of the erythrocyte membrane. Biochem J. 1962 Sep;84:611–621. doi: 10.1042/bj0840611. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DINGLE J. T., LUCY J. A. VITAMIN A, CAROTENOIDS AND CELL FUNCTION. Biol Rev Camb Philos Soc. 1965 Aug;40:422–461. doi: 10.1111/j.1469-185x.1965.tb00809.x. [DOI] [PubMed] [Google Scholar]
  9. FELL H. B., MELLANBY E. Metaplasia produced in cultures of chick ectoderm by high vitamin A. J Physiol. 1953 Mar;119(4):470–488. doi: 10.1113/jphysiol.1953.sp004860. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FELL H. B., MELLANBY E. The effect of hypervitaminosis A on embryonic limb bones cultivated in vitro. J Physiol. 1952 Mar;116(3):320–349. doi: 10.1113/jphysiol.1952.sp004708. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GLAUERT A. M., DANIEL M. R., LUCY J. A., DINGLE J. T. Studies on the mode of action of excess of vitamin A. VII. Changes in the fine structure of erythrocytes during haemolysis by vitamin A. J Cell Biol. 1963 Apr;17:111–121. doi: 10.1083/jcb.17.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HEYNER S. IN SITU EMBEDDING OF CULTURED CELLS OR TISSUE, GROWN ON GLASS, IN EPOXY RESINS FOR ELECTRON MICROSCOPY. Stain Technol. 1963 Nov;38:335–336. [PubMed] [Google Scholar]
  13. KARRER H. E. Electron microscopic study of the phagocytosis process in lung. J Biophys Biochem Cytol. 1960 Apr;7:357–366. doi: 10.1083/jcb.7.2.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LUCY J. A., DINGLE J. T. FAT-SOLUBLE VITAMINS AND BIOLOGICAL MEMBRANES. Nature. 1964 Oct 10;204:156–160. doi: 10.1038/204156a0. [DOI] [PubMed] [Google Scholar]
  15. LUCY J. A., LUSCOMBE M., DINGLE J. T. STUDIES ON THE MODE OF ACTION OF EXCESS OF VITAMIN A. 8. MITOCHONDRIAL SWELLING. Biochem J. 1963 Dec;89:419–425. doi: 10.1042/bj0890419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MILLONIG G. A modified procedure for lead staining of thin sections. J Biophys Biochem Cytol. 1961 Dec;11:736–739. doi: 10.1083/jcb.11.3.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. NOVIKOFF A. B., ESSNER E. Cytolysomes and mitochondrial degeneration. J Cell Biol. 1962 Oct;15:140–146. doi: 10.1083/jcb.15.1.140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. NOVIKOFF A. B., ESSNER E., QUINTANA N. GOLGI APPARATUS AND LYSOSOMES. Fed Proc. 1964 Sep-Oct;23:1010–1022. [PubMed] [Google Scholar]
  19. PAUL J., PEARSON E. S. The action of insulin on the metabolism of cell cultures. J Endocrinol. 1960 Dec;21:287–294. doi: 10.1677/joe.0.0210287. [DOI] [PubMed] [Google Scholar]
  20. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. WADDINGTON C. H., PERRY M. M. Helical arrangement of ribosomes in differentiating muscle cells. Exp Cell Res. 1963 May;30:599–600. doi: 10.1016/0014-4827(63)90339-2. [DOI] [PubMed] [Google Scholar]
  23. YASUZUMI G., SUGIHARA R., NAKANO S., KISE T., TAKEUCHI H. Submicroscopic structure of cell necrobiosis of Yoshida sarcoma as revealed by electron microscopy. Cancer Res. 1960 Apr;20:339–343. [PubMed] [Google Scholar]

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