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. 1988 Feb;170(2):653–656. doi: 10.1128/jb.170.2.653-656.1988

Genetic transformation system in the archaebacterium Methanobacterium thermoautotrophicum Marburg.

V E Worrell 1, D P Nagle Jr 1, D McCarthy 1, A Eisenbraun 1
PMCID: PMC210704  PMID: 3422229

Abstract

A wild-type strain of Methanobacterium thermoautotrophicum Marburg was transformed by DNA from strains resistant to 5-fluorouracil. Recipient cells were grown without selection on gellan gum (GELRITE) plates with DNA. Drug-resistant cells were recovered by replica plating the resulting colonies onto drug plates. Transformation required high-molecular-weight DNA with appropriate markers and was not observed on agar or in liquid media under a variety of conditions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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