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. 1966 Oct 1;31(1):95–105. doi: 10.1083/jcb.31.1.95

ISOLATION OF METAPHASE CHROMOSOMES FROM HELA CELLS

Joel A Huberman 1, Giuseppe Attardi 1
PMCID: PMC2107040  PMID: 5339564

Abstract

The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically intact chromosomes can be isolated in 8 hr or less of total working time. The isolated chromosomes contain about 2.0 mg of acid-soluble protein, 2.7 mg of acid-insoluble protein and 0.66 mg of RNA for each milligram of DNA. The RNA bound to the isolated chromosomes consists mainly of ribosomal RNA, but there is also a significant amount of 45S RNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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