Skip to main content
NCBI home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1966 Nov 1;31(2):243–256. doi: 10.1083/jcb.31.2.243

ON THE RELATIONSHIP OF LIVER GLUCOSE-6-PHOSPHATASE TO THE PROLIFERATION OF ENDOPLASMIC RETICULUM IN PHENOBARBITAL INDUCTION

Sten Orrenius 1, Jan L E Ericsson 1
PMCID: PMC2107056  PMID: 19866699

Abstract

The differentiated effects of phenobarbital treatment on liver microsomal enzymes have been further studied. The relationship between the resulting decrease in the specific glucose-6-phosphatase activity and the enhancement of formation of endoplasmic reticulum membranes with high drug-hydroxylating activity has been investigated with biochemical and histochemical methods. Biochemically and histochemically demonstrable glucose-6-phosphatase activity was found to be present in all endoplasmic reticulum membranes, including the phenobarbital-induced smooth-surfaced proliferates, even though there was an over-all decrease in activity. Actinomycin D did not inhibit the decrease in glucose-6-phosphatase activity. The findings are discussed with reference to the enzyme-membrane relationship in phenobarbital induction.

Full Text

The Full Text of this article is available as a PDF (1.8 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENNETT H. S., LUFT J. H. zeta-Collidine as a basis for buffering fixatives. J Biophys Biochem Cytol. 1959 Aug;6(1):113–114. doi: 10.1083/jcb.6.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHRISTENSEN A. K., FAWCETT D. W. The normal fine structure of opossum testicular interstitial cells. J Biophys Biochem Cytol. 1961 Mar;9:653–670. doi: 10.1083/jcb.9.3.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CONNEY A. H., DAVISON C., GASTEL R., BURNS J. J. Adaptive increases in drug-metabolizing enzymes induced by phenobarbital and other drugs. J Pharmacol Exp Ther. 1960 Sep;130:1–8. [PubMed] [Google Scholar]
  4. CRABO B. FINE STRUCTURE OF THE INTERSTITIAL CELLS OF THE RABBIT TESTES. Z Zellforsch Mikrosk Anat. 1963 Dec 3;61:587–604. doi: 10.1007/BF00344002. [DOI] [PubMed] [Google Scholar]
  5. Ericsson J. L. On the fine structural demonstration of glucose 6-phosphatase. J Histochem Cytochem. 1966 Apr;14(4):361–362. doi: 10.1177/14.4.361. [DOI] [PubMed] [Google Scholar]
  6. Jones A. L., Fawcett D. W. Hypertrophy of the agranular endoplasmic reticulum in hamster liver induced by phenobarbital (with a review on the functions of this organelle in liver). J Histochem Cytochem. 1966 Mar;14(3):215–232. doi: 10.1177/14.3.215. [DOI] [PubMed] [Google Scholar]
  7. KARNOVSKY M. J. Simple methods for "staining with lead" at high pH in electron microscopy. J Biophys Biochem Cytol. 1961 Dec;11:729–732. doi: 10.1083/jcb.11.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Orrenius S., Ericsson J. L. Enzyme-membrane relationship in phenobarbital induction of synthesis of drug-metabolizing enzyme system and proliferation of endoplasmic membranes. J Cell Biol. 1966 Feb;28(2):181–198. doi: 10.1083/jcb.28.2.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Orrenius S., Ericsson J. L., Ernster L. Phenobarbital-induced synthesis of the microsomal drug-metabolizing enzyme system and its relationship to the proliferation of endoplasmic membranes. A morphological and biochemical study. J Cell Biol. 1965 Jun;25(3):627–639. doi: 10.1083/jcb.25.3.627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. REMMER H. Der beschleunigte Abbau von Pharmaka in den Lebermikrosomen unter dem Einfluss von Luminal. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1959;235(4):279–290. [PubMed] [Google Scholar]
  12. REMMER H., MERKER H. J. DRUG-INDUCED CHANGES IN THE LIVER ENDOPLASMIC RETICULUM: ASSOCIATION WITH DRUG-METABOLIZING ENZYMES. Science. 1963 Dec 27;142(3600):1657–1658. doi: 10.1126/science.142.3600.1657. [DOI] [PubMed] [Google Scholar]
  13. REMMER H., MERKER H. J. [Enzyme induction and increase of endoplasmic reticulum in liver cells during phenobarbital (Luminal) therapy]. Klin Wochenschr. 1963 Mar 15;41:276–282. doi: 10.1007/BF01483392. [DOI] [PubMed] [Google Scholar]
  14. Rosen S. I., Kelly G. W., Peters V. B. Glucose-6-phosphatase in tubular endoplasmic reticulum of hepatocytes. Science. 1966 Apr 15;152(3720):352–354. doi: 10.1126/science.152.3720.352. [DOI] [PubMed] [Google Scholar]
  15. SPAIN J. D. Precancerous metabolic alterations in the process of Azo dye carcinogenesis. Tex Rep Biol Med. 1956;14(4):528–537. [PubMed] [Google Scholar]
  16. TRUMP B. F., ERICSSON J. L. THE EFFECT OF THE FIXATIVE SOLUTION ON THE ULTRASTRUCTURE OF CELLS AND TISSUES. A COMPARATIVE ANALYSIS WITH PARTICULAR ATTENTION TO THE PROXIMAL CONVOLUTED TUBULE OF THE RAT KIDNEY. Lab Invest. 1965 Jun;14:1245–1323. [PubMed] [Google Scholar]
  17. TRUMP B. F., GOLDBLATT P. J., STOWELL R. E. An electron microscopic study of early cytoplasmic alterations in hepatic parenchymal cells of mouse liver during necrosis in vitro (autolysis). Lab Invest. 1962 Nov;11:986–1015. [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES