Abstract
The structural changes during spermiogenesis were studied on developing spermatids in seminal vesicles and receptacles of Lumbricus terrestris fixed in glutaraldehyde-osmium tetroxide and embedded in Epon-Araldite. The centriole plays a prominent role in the morphogenesis and organization of the microtubules of the manchette and flagellum. Microtubules arising from the centriole extend anteriorly to encase the developing middle piece, the nucleus, and the acrosome. The manchette not only provides a supporting framework for the cell during elongation, but also may provide the motive force for the elimination of both nucleoplasm and cytoplasm. The manchette participates in segregation and elimination of the nuclear vesicle that contains the nonchromatin nucleoplasm. Compartmentalization and conservation may also be a function of the manchette since those elements which remain within the framework of microtubules are retained, while all the cytoplasm outside the manchette is discarded. At maturation, the endoplasmic reticulum plays a key role in dismantling the manchette and reducing the cytoplasm external to it. During the early stages of middle-piece formation, six ovoid mitochondria aggregate at the posterior pole of the spermatid nucleus. Concurrent with manchette formation, the mitochondria are compressed laterally into elongate wedge-shaped components, and their outer limiting membranes fuse to form an hexagonal framework that surrounds the dense intramitochondrial matrices. Dense glycogen granules are arranged linearly between the peripheral flagellar tubules and the outer membrane of the mature sperm tail.
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Selected References
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- BURGOS M. H., FAWCETT D. W. Studies on the fine structure of the mammalian testis. I. Differentiation of the spermatids in the cat (Felis domestica). J Biophys Biochem Cytol. 1955 Jul 25;1(4):287–300. doi: 10.1083/jcb.1.4.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BYERS B., PORTER K. R. ORIENTED MICROTUBULES IN ELONGATING CELLS OF THE DEVELOPING LENS RUDIMENT AFTER INDUCTION. Proc Natl Acad Sci U S A. 1964 Oct;52:1091–1099. doi: 10.1073/pnas.52.4.1091. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lane B. P., Europa D. L. Differential staining of ultrathin sections of Epon-embedded tissues for light microscopy. J Histochem Cytochem. 1965 Sep-Oct;13(7):579–582. doi: 10.1177/13.7.579. [DOI] [PubMed] [Google Scholar]
- NAGANO T. Observations on the fine structure of the developing spermatid in the domestic chicken. J Cell Biol. 1962 Aug;14:193–205. doi: 10.1083/jcb.14.2.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RENAUD F. L., SWIFT H. THE DEVELOPMENT OF BASAL BODIES AND FLAGELLA IN ALLOMYCES ARBUSCULUS. J Cell Biol. 1964 Nov;23:339–354. doi: 10.1083/jcb.23.2.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROBBINS E., GONATAS N. K. THE ULTRASTRUCTURE OF A MAMMALIAN CELL DURING THE MITOTIC CYCLE. J Cell Biol. 1964 Jun;21:429–463. doi: 10.1083/jcb.21.3.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SZOLLOSI D. THE STRUCTURE AND FUNCTION OF CENTRIOLES AND THEIR SATELLITES IN THE JELLYFISH PHIALIDIUM GREGARIUM. J Cell Biol. 1964 Jun;21:465–479. doi: 10.1083/jcb.21.3.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tandler B., Moriber L. G. Microtubular structures associated with the acrosome during spermiogenesis in the water-strider, Gerris remigis (Say). J Ultrastruct Res. 1966 Feb;14(3):391–404. doi: 10.1016/s0022-5320(66)80056-4. [DOI] [PubMed] [Google Scholar]
- VOELZ H., DWORKIN M. Fine structure of Myxococcus xanthus during morphogenesis. J Bacteriol. 1962 Nov;84:943–952. doi: 10.1128/jb.84.5.943-952.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YASUZUMI G., TANAKA H. Spermatogenesis in animals as revealed by electron microscopy. VI. Researches on the spermatozoon-dimorphism in a pond snail, Cipango-paludina malleata. J Biophys Biochem Cytol. 1958 Sep 25;4(5):621–630. doi: 10.1083/jcb.4.5.621. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yasuzumi G., Oura C. Spermatogenesis in animals as revealed by electron microscopy. XV. The fine structure of the middle piece in the developing spermatid of the silkworm, Bombyx mori Linné. Z Zellforsch Mikrosk Anat. 1965 Aug 5;67(4):502–520. [PubMed] [Google Scholar]