Abstract
The distribution and localization of structural polymers in the cell wall of Neurospora crassa has been studied by selective removal and light and electron microscope examination. Observations with the light microscope indicated that each polymer by itself can provide structural integrity to the cell wall. Examination by electron microscopy showed that the cell wall consists of an outer layer of thick fibrils, identified chemically as a glucan-peptide-galactosamine complex, and an inner layer made up of β-1,3 glucan and thin fibrils of chitin.
Full Text
The Full Text of this article is available as a PDF (699.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- GORNALL A. G., BARDAWILL C. J., DAVID M. M. Determination of serum proteins by means of the biuret reaction. J Biol Chem. 1949 Feb;177(2):751–766. [PubMed] [Google Scholar]
- HAWKER L. E. FINE STRUCTURE OF FUNGI AS REVEALED BY ELECTRON MICROSCOPY. Biol Rev Camb Philos Soc. 1965 Feb;40:52–92. doi: 10.1111/j.1469-185x.1965.tb00795.x. [DOI] [PubMed] [Google Scholar]
- HORIKOSHI K., IIDA S. STUDIES OF THE SPORE COATS OF FUNGI. I. ISOLATION AND COMPOSITION OF THE SPORE COATS OF ASPERGILLUS ORYZAE. Biochim Biophys Acta. 1964 Jul 7;83:197–203. doi: 10.1016/0926-6526(64)90035-7. [DOI] [PubMed] [Google Scholar]
- KELLENBERGER E., RYTER A., SECHAUD J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol. 1958 Nov 25;4(6):671–678. doi: 10.1083/jcb.4.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mahadevan P. R., Tatum E. L. Relationship of the major constituents of the Neurospora crassa cell wall to wild-type and colonial morphology. J Bacteriol. 1965 Oct;90(4):1073–1081. doi: 10.1128/jb.90.4.1073-1081.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NORTHCOTE D. H., HORNE R. W. The chemical composition and structure of the yeast cell wall. Biochem J. 1952 May;51(2):232–236. doi: 10.1042/bj0510232. [DOI] [PMC free article] [PubMed] [Google Scholar]
- POTGIETER H. J., ALEXANDER M. POLYSACCHARIDE COMPONENTS OF NEUROSPORA CRASSA HYPHAL WALLS. Can J Microbiol. 1965 Feb;11:122–125. doi: 10.1139/m65-017. [DOI] [PubMed] [Google Scholar]
- RONDLE C. J., MORGAN W. T. The determination of glucosamine and galactosamine. Biochem J. 1955 Dec;61(4):586–589. doi: 10.1042/bj0610586. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SHATKIN A. J., TATUM E. L. Electron microscopy of Neurospora crassa mycelia. J Biophys Biochem Cytol. 1959 Dec;6:423–426. doi: 10.1083/jcb.6.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Skujins J. J., Potgieter H. J., Alexander M. Dissolution of fungal cell walls by a streptomycete chitinase and beta-(1-3) glucanase. Arch Biochem Biophys. 1965 Aug;111(2):358–364. doi: 10.1016/0003-9861(65)90197-9. [DOI] [PubMed] [Google Scholar]
- TREVELYAN W. E., HARRISON J. S. Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates. Biochem J. 1952 Jan;50(3):298–303. doi: 10.1042/bj0500298. [DOI] [PMC free article] [PubMed] [Google Scholar]
