Abstract
Samples of mycelium of Neurospora crassa of known age were harvested from agar plates and examined with the electron microscope. The relative volume of the mitochondria was determined for mycelium of different ages. The volume measurements indicated that the mitochondria were dividing synchronously in fronts 6, 13, and 22½ hr behind the growing hyphal tips. The sequence of mitochondrial division is hypothesized to include mitochondrial cupping followed by division which results in closely associated daughter mitochondria. On the basis of percentages of mitochondrial cupping and association, mitochondrial division was postulated to be occurring at 6, 14, and 26 hr. Close agreement between the mycelial mass doubling time and the calculated mitochondrial mass doubling time indicates that synchronous mitochondrial division is sufficient to maintain growth. The possibility that mitochondrial division is due to intercellular regulation of a mitochondrial genetic system is advanced.
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- Avers C. J., Rancourt M. W., Lin F. H. Intracellular mitochondrial diversity in various strains of Saccgarintces cerevisiae. Proc Natl Acad Sci U S A. 1965 Aug;54(2):527–535. doi: 10.1073/pnas.54.2.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BAHR G. F., ZEITLER E. Study of mitochondria in rat liver. Quantitative electron microscopy. J Cell Biol. 1962 Dec;15:489–501. doi: 10.1083/jcb.15.3.489. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clark-Walker G. D., Linnane A. W. In vivo differentiation of yeast cytoplasmic and mitochondrial protein synthesis with antibiotics. Biochem Biophys Res Commun. 1966 Oct 5;25(1):8–13. doi: 10.1016/0006-291x(66)90631-0. [DOI] [PubMed] [Google Scholar]
- LAFONTAINE J. G., ALLARD C. A LIGHT AND ELECTRON MICROSCOPE STUDY OF THE MORPHOLOGICAL CHANGES INDUCED IN RAT LIVER CELLS BY THE AZO DYE 2-ME-DAB. J Cell Biol. 1964 Jul;22:143–172. doi: 10.1083/jcb.22.1.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUCK D. F. FORMATION OF MITOCHONDRIA IN NEUROSPORA CRASSA. A STUDY BASED ON MITOCHONDRIAL DENSITY CHANGES. J Cell Biol. 1965 Mar;24:461–470. doi: 10.1083/jcb.24.3.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUCK D. J. Formation of mitochondria in Neurospora crassa. A quantitative radioautographic study. J Cell Biol. 1963 Mar;16:483–499. doi: 10.1083/jcb.16.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUCK D. J. Genesis of mitochondria in neurospora crassa. Proc Natl Acad Sci U S A. 1963 Feb 15;49:233–240. doi: 10.1073/pnas.49.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUCK D. J., REICH E. DNA IN MITOCHONDRIA OF NEUROSPORA CRASSA. Proc Natl Acad Sci U S A. 1964 Oct;52:931–938. doi: 10.1073/pnas.52.4.931. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mounolou J. C., Jakob H., Slonimski P. P. Mitochondrial DNA from yeast "petite" mutants: specific changes in buoyant density corresponding to different cytoplasmic mutations. Biochem Biophys Res Commun. 1966 Jul 20;24(2):218–224. doi: 10.1016/0006-291x(66)90723-6. [DOI] [PubMed] [Google Scholar]
- NASS M. M., NASS S., AFZELIUS B. A. THE GENERAL OCCURENCE OF MITOCHONDRIAL DNA. Exp Cell Res. 1965 Mar;37:516–539. doi: 10.1016/0014-4827(65)90204-1. [DOI] [PubMed] [Google Scholar]
- NASS M. M., NASS S. INTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS. I. FIXATION AND ELECTRON STAINING REACTIONS. J Cell Biol. 1963 Dec;19:593–611. doi: 10.1083/jcb.19.3.593. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NASS S., NASS M. M. INTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS. II. ENZYMATIC AND OTHER HYDROLYTIC TREATMENTS. J Cell Biol. 1963 Dec;19:613–629. doi: 10.1083/jcb.19.3.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reich E., Luck D. J. Replication and inheritance of mitochondrial DNA. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1600–1608. doi: 10.1073/pnas.55.6.1600. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schuster F. L. A deoxyribose nucleic acid component in mitochondria of Didymium nigripes, a slime mold. Exp Cell Res. 1965 Sep;39(2):329–345. doi: 10.1016/0014-4827(65)90038-8. [DOI] [PubMed] [Google Scholar]
- Vogel F. S., Kemper L. Structural and functional characteristics of deoxyribonucleic acid-rich mitochondria of the common meadow mushroom, Agaricus campestris. II. Extracellular cultures. Lab Invest. 1965 Nov;14(11):1868–1893. [PubMed] [Google Scholar]
- Vogel F. S. Structural and functional characteristics of deoxyribonucleic acid-rich mitochondria of the common meadow mushroom, Agaricus campestris. I. Intracellular environment. Lab Invest. 1965 Nov;14(11):1849–1867. [PubMed] [Google Scholar]
- WAGNER R. P., BERGQUIST A. Synthesis of valine and isoleucine in the presence of a particulate cell fraction of Neurospora. Proc Natl Acad Sci U S A. 1963 Jun;49:892–897. doi: 10.1073/pnas.49.6.892. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEISS B. AN ELECTRON MICROSCOPE AND BIOCHEMICAL STUDY OF NEUROSPORA CRASSA DURING DEVELOPMENT. J Gen Microbiol. 1965 Apr;39:85–94. doi: 10.1099/00221287-39-1-85. [DOI] [PubMed] [Google Scholar]
- Wintersberger E. Occurrence of a DNA-polymerase in isolated yeast mitochondria. Biochem Biophys Res Commun. 1966 Oct 5;25(1):1–7. doi: 10.1016/0006-291x(66)90630-9. [DOI] [PubMed] [Google Scholar]
- Woodward D. O., Munkres K. D. Alterations of a maternally inherited mitochondrial structural protein in respiratory-deficient strains of Neurospora. Proc Natl Acad Sci U S A. 1966 Apr;55(4):872–880. doi: 10.1073/pnas.55.4.872. [DOI] [PMC free article] [PubMed] [Google Scholar]