Abstract
Proliferation of Schwann cells in neonatal mouse sciatic nerve was studied radioautographically in 1-µ glycol methacrylate sections. 28 mice were injected with thymidine-3H, 4 µc/g, 48 hr after birth, and were killed serially over the next 4 days. For the cell cycle following injection, the generation time was approximately 24 hr as determined by grain-count halving data; the duration of synthesis phase was 8 hr as determined from a curve constructed from the per cent of mitotic figures containing label; and the labeling index was 9% at 2 hr after injection. With these estimates, the per cent of Schwann cells proliferating was calculated to be 27%. In addition, roughly 25% of dividing cells appeared to cease division during the cell cycle under study. The relationship of these findings to other events during maturation of nerve is discussed.
Full Text
The Full Text of this article is available as a PDF (538.7 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ashley C. A., Feder N. Glycol methacrylate in histopathology. A study of central necrosis of the liver using a water-miscible plastic as embedding medium. Arch Pathol. 1966 May;81(5):391–397. [PubMed] [Google Scholar]
- BRYANT B. J. THE DELAYED UPTAKE OF 3H-THYMIDINE BY EHRLICH ASCITES TUMOR CELLS. Exp Cell Res. 1965 Feb;37:490–494. doi: 10.1016/0014-4827(65)90198-9. [DOI] [PubMed] [Google Scholar]
- CAUSEY G., BARTON A. A. The cellular content of the endoneurium of peripheral nerve. Brain. 1959 Dec;82:594–598. doi: 10.1093/brain/82.4.594. [DOI] [PubMed] [Google Scholar]
- DIDERHOLM H., FICHTELIUS K. E., LINDER O. Availability time of 3H-label after administration of 3H-thymidine in vivo. Exp Cell Res. 1962 Sep;27:431–435. doi: 10.1016/0014-4827(62)90008-3. [DOI] [PubMed] [Google Scholar]
- Diner O. Les cellules de Schwann en mitose et leurs rapports avec les axones au cours du développement du nerf sciatique chez le rat. C R Acad Sci Hebd Seances Acad Sci D. 1965 Aug 18;261(7):1731–1734. [PubMed] [Google Scholar]
- Feinendegen L. E., Bond V. P., Hughes W. L. Physiological thymidine reutilization in rat bone marrow. Proc Soc Exp Biol Med. 1966 Jun;122(2):448–455. doi: 10.3181/00379727-122-31159. [DOI] [PubMed] [Google Scholar]
- PETERS A., MUIR A. R. The relationship between axons and Schwann cells during development of peripheral nerves in the rat. Q J Exp Physiol Cogn Med Sci. 1959 Jan;44(1):117–130. doi: 10.1113/expphysiol.1959.sp001366. [DOI] [PubMed] [Google Scholar]
- QUASTLER H., SHERMAN F. G. Cell population kinetics in the intestinal epithelium of the mouse. Exp Cell Res. 1959 Jun;17(3):420–438. doi: 10.1016/0014-4827(59)90063-1. [DOI] [PubMed] [Google Scholar]
- ROBINSON S. H., BRECHER G. DELAYED INCORPORATION OF TRITIATED THYMIDINE INTO DNA. Science. 1963 Oct 18;142(3590):392–393. doi: 10.1126/science.142.3590.392. [DOI] [PubMed] [Google Scholar]
- THOMAS P. K. The connective tissue of peripheral nerve: an electron microscope study. J Anat. 1963 Jan;97:35–44. [PMC free article] [PubMed] [Google Scholar]
- WESTON J. A. A radioautographic analysis of the migration and localization of trunk neural crest cells in the chick. Dev Biol. 1963 Jun;6:279–310. doi: 10.1016/0012-1606(63)90016-2. [DOI] [PubMed] [Google Scholar]
- Webster H. D., Schröder J. M., Asbury A. K., Adams R. D. The role of Schwann cells in the formation of "onion bulbs" found in chronic neuropathies. J Neuropathol Exp Neurol. 1967 Apr;26(2):276–299. doi: 10.1097/00005072-196704000-00008. [DOI] [PubMed] [Google Scholar]