Abstract
The fine structure of myelin was studied in glutaraldehyde-fixed rat sciatic nerves depleted of lipid by acetone, chloroform:methanol (2:1 v/v), and chloroform:methanol:concentrated HCl (200:100:1, v/v/v). One portion of each of these nerves, plus the extracts, was saponified and analyzed by gas-liquid chromatography for fatty acids. The remainder of each nerve was stained in osmium tetroxide in CCl4 (5g/100cc) and was embedded in Epon 812. Thin sections, examined in the electron microscope, revealed the preservation of myelin lamellar structure with a 170 A periodicity in nerves depleted of 98% of their lipids. Preservation of myelin lamellar structure depended on glutaraldehyde fixation and the introduction of osmium tetroxide in a nonpolar vehicle (CCl4) after the lipids had been extracted. It is concluded that the periodic lamellar structure in electron micrographs of myelin depleted of lipid results from the complexing of osmium tetroxide, plus uranyl and lead stains, with protein.
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- BEN GEREN B. The formation from the Schwann cell surface of myelin in the peripheral nerves of chick embryos. Exp Cell Res. 1954 Nov;7(2):558–562. doi: 10.1016/s0014-4827(54)80098-x. [DOI] [PubMed] [Google Scholar]
- FINEAN J. B. The nature and stability of nerve myelin. Int Rev Cytol. 1961;12:303–336. doi: 10.1016/s0074-7696(08)60543-4. [DOI] [PubMed] [Google Scholar]
- Finean J. B., Martonosi A. The action of phospholipase C on muscle microsomes: a correlation of electron microscope and biochemical data. Biochim Biophys Acta. 1965 Jun 1;98(3):547–553. doi: 10.1016/0005-2760(65)90151-7. [DOI] [PubMed] [Google Scholar]
- GIBBONS I. R., GRIMSTONE A. V. On flagellar structure in certain flagellates. J Biophys Biochem Cytol. 1960 Jul;7:697–716. doi: 10.1083/jcb.7.4.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karlsson U. Comparison of the myelin period of peripheral and central origin by electron microscopy. J Ultrastruct Res. 1966 Jun;15(3):451–468. doi: 10.1016/s0022-5320(66)80118-1. [DOI] [PubMed] [Google Scholar]
- Korn E. D. Structure of biological membranes. Science. 1966 Sep 23;153(3743):1491–1498. doi: 10.1126/science.153.3743.1491. [DOI] [PubMed] [Google Scholar]
- LEBARON F. N. THE NATURE OF THE LINKAGE BETWEEN PHOSPHOINOSITIDES AND PROTEINS IN BRAIN. Biochim Biophys Acta. 1963 Dec 27;70:658–669. doi: 10.1016/0006-3002(63)90810-2. [DOI] [PubMed] [Google Scholar]
- MILCH R. A. REACTION OF CERTAIN ALIPHATIC ALDEHYDES WITH GELATIN. Gerontologia. 1964;10:117–136. doi: 10.1159/000211399. [DOI] [PubMed] [Google Scholar]
- Maddy A. H. The chemical organization of the plasma membrane of animal cells. Int Rev Cytol. 1966;20:1–65. doi: 10.1016/s0074-7696(08)60796-2. [DOI] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rumsby M. G., Finean J. B. The action of organic solvents on the myelin sheath of peripheral nerve tissue. I. Methanol, ethanol, chloroform and chloroform-methanol (2:1, v-v). J Neurochem. 1966 Dec;13(12):1501–1507. doi: 10.1111/j.1471-4159.1966.tb04311.x. [DOI] [PubMed] [Google Scholar]
- Sorokin S P. A morphologic and cytochemical study on the great alveolar cell. J Histochem Cytochem. 1966 Dec;14(12):884–897. doi: 10.1177/14.12.884. [DOI] [PubMed] [Google Scholar]