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. 1967 Jun 1;33(3):605–623. doi: 10.1083/jcb.33.3.605

INTRAMITOCHONDRIAL FILAMENTOUS BODIES IN THE THICK LIMB OF HENLE OF THE RAT KIDNEY

Teruo Suzuki 1, F K Mostofi 1
PMCID: PMC2107209  PMID: 6036524

Abstract

"Intramitochondrial filamentous bodies" (IMFB) were occasionally found within the matrix of some mitochondria of the thick limb of Henle of the rat kidney, but not elsewhere in the tubular system. Three types were recognized: type I, an accumulation of filaments 55 A thick; type II, a bundle of parallel filaments having the same thickness as those of type I and regular spacing, 87 A apart, from center to center; and type III, consisting of type II with regular light bands of 280 A periodicity and a helical border of prismatic tubular cristae. In addition to these, electron-opaque masses showing variable and faint substructures were found in the matrix of mitochondria. It is suggested that all these IMFB may originate from mitochondrial cristae and that type II IMFB may be an intermediate developmental form between type I and type III. After uranyl acetate staining, IMFB and the membranes of prismatic tubular cristae showed highly increased electron opacity. The literature has been reviewed for reports of intramitochondrial filamentous inclusions in various types of cells. These inclusions have been classified according to their structural characteristics and the localization in the mitochondria and compared with IMFB reported herein.

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Selected References

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  1. ADAMS E. C., HERTIG A. T. STUDIES ON GUINEA PIG OOCYTES. I. ELECTRON MICROSCOPIC OBSERVATIONS ON THE DEVELOPMENT OF CYTOPLASMIC ORGANELLES IN OOCYTES OF PRIMORDIAL AND PRIMARY FOLLICLES. J Cell Biol. 1964 Jun;21:397–427. doi: 10.1083/jcb.21.3.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ANDERSON E., BEAMS H. W. Cytological observations on the fine structure of the guinea pig ovary with special reference to the oogonium, primary oocyte and associated follicle cells. J Ultrastruct Res. 1960 Jun;3:432–446. doi: 10.1016/s0022-5320(60)90021-6. [DOI] [PubMed] [Google Scholar]
  3. BLINZINGER K., REWCASTLE N. B., HAGER H. OBSERVATIONS ON PRISMATIC-TYPE MITOCHONDRIA WITHIN ASTROCYTES OF THE SYRIAN HAMSTER BRAIN. J Cell Biol. 1965 May;25:293–303. doi: 10.1083/jcb.25.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Battifora H., Eisenstein R., Laing G. H., McCreary P. The kidney in experimental magnesium deprivation. a morphologic and biochemical study. Am J Pathol. 1966 Mar;48(3):421–437. [PMC free article] [PubMed] [Google Scholar]
  5. CLAUDE A. A spontaneous, transplantable renal carcinoma of the mouse. Electron microscope study of the cells and of an associated virus-like particle. J Ultrastruct Res. 1962 Feb;6:1–18. doi: 10.1016/s0022-5320(62)90057-6. [DOI] [PubMed] [Google Scholar]
  6. ELLIOTT A. M., BAK I. J. THE FATE OF MITOCHONDRIA DURING AGING IN TETRAHYMENA PYRIFORMIS. J Cell Biol. 1964 Jan;20:113–129. doi: 10.1083/jcb.20.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FAWCETT D. W., ITO S. Observations on the cytoplasmic membranes of testicular cells, examined by phase contrast and electron microscopy. J Biophys Biochem Cytol. 1958 Mar 25;4(2):135–142. doi: 10.1083/jcb.4.2.135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FAWCETT D. W. The membranes of the cytoplasm. Lab Invest. 1961 Nov-Dec;10:1162–1188. [PubMed] [Google Scholar]
  9. FINEAN J. B. Electron microscope and x-ray diffraction studies of a saturated synthetic phospholipide. J Biophys Biochem Cytol. 1959 Aug;6(1):123–124. doi: 10.1083/jcb.6.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. FREI J. V., SHELDON H. Corpus intra cristam: a dense body within mitochondria of cells in hyperplastic mouse epidermis. J Biophys Biochem Cytol. 1961 Dec;11:724–729. doi: 10.1083/jcb.11.3.724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. FUJITA H., MACHINO M. FINE STRUCTURE OF INTRAMITOCHONDRIAL CRYSTALS IN RAT THYROID FOLLICULAR CELL. J Cell Biol. 1964 Nov;23:383–385. doi: 10.1083/jcb.23.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HUXLEY H. E., ZUBAY G. Preferential staining of nucleic acid-containing structures for electron microscopy. J Biophys Biochem Cytol. 1961 Nov;11:273–296. doi: 10.1083/jcb.11.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Iseri O. A., Lieber C. S., Gottlieb L. S. The ultrastructure of fatty liver induced by prolonged ethanol ingestion. Am J Pathol. 1966 Apr;48(4):535–555. [PMC free article] [PubMed] [Google Scholar]
  14. LAGUENS R., BIANCHI N. Fine structure of the liver in human idiopathic diabetes mellitus. I. Parenchymal cell mitochondria. Exp Mol Pathol. 1963 Jun;2:203–214. doi: 10.1016/0014-4800(63)90035-2. [DOI] [PubMed] [Google Scholar]
  15. LUFT R., IKKOS D., PALMIERI G., ERNSTER L., AFZELIUS B. A case of severe hypermetabolism of nonthyroid origin with a defect in the maintenance of mitochondrial respiratory control: a correlated clinical, biochemical, and morphological study. J Clin Invest. 1962 Sep;41:1776–1804. doi: 10.1172/JCI104637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MILLONIG G. A modified procedure for lead staining of thin sections. J Biophys Biochem Cytol. 1961 Dec;11:736–739. doi: 10.1083/jcb.11.3.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MUGNAINI E. HELICAL FILAMENTS IN ASTROCYTIC MITOCHONDRIA OF THE CORPUS STRIATUM IN THE RAT. J Cell Biol. 1964 Oct;23:173–182. doi: 10.1083/jcb.23.1.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. MUGNAINI E., WALBERG F. ULTRASTRUCTURE OF NEUROGLIA. Ergeb Anat Entwicklungsgesch. 1964;37:194–236. [PubMed] [Google Scholar]
  19. NAPOLITANO L., FAWCETT D. The fine structure of brown adipose tissue in the newborn mouse and rat. J Biophys Biochem Cytol. 1958 Nov 25;4(6):685–692. doi: 10.1083/jcb.4.6.685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. NASS M. M., NASS S. Fibrous structures within the matrix of developing chick embryo mitochondria. Exp Cell Res. 1962 Mar;26:424–427. doi: 10.1016/0014-4827(62)90194-5. [DOI] [PubMed] [Google Scholar]
  21. NASS M. M., NASS S. INTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS. I. FIXATION AND ELECTRON STAINING REACTIONS. J Cell Biol. 1963 Dec;19:593–611. doi: 10.1083/jcb.19.3.593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. NASS S., NASS M. M. INTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS. II. ENZYMATIC AND OTHER HYDROLYTIC TREATMENTS. J Cell Biol. 1963 Dec;19:613–629. doi: 10.1083/jcb.19.3.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. PAPPAS G. D., BRANDT P. W. Mitochondria. I. Fine structure of the complex patterns in the mitochondria of Pelomyxa carolinensis Wilson (Chaos chaos L.). J Biophys Biochem Cytol. 1959 Aug;6(1):85–90. doi: 10.1083/jcb.6.1.85. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Porta E. A., Hartroft W. S., De la Iglesia F. A. Hepatic changes associated with chronic alcoholism in rats. Lab Invest. 1965 Aug;14(8):1437–1455. [PubMed] [Google Scholar]
  25. REICHEL J., GOLDBERG S. B., ELLENBERG M., SCHAFFNER F. Intrahepatic cholestasis following administration of chlorpropamide. Report of a case with electron microscopic observations. Am J Med. 1960 Apr;28:654–660. doi: 10.1016/0002-9343(60)90161-3. [DOI] [PubMed] [Google Scholar]
  26. REVEL J. P., FAWCETT D. W., PHILPOTT C. W. Observations on mitochondrial structure angular configurations of the cristae. J Cell Biol. 1963 Jan;16:187–195. doi: 10.1083/jcb.16.1.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. SCHAFFNER F., LOEBEL A., WEINER H. A., BARKA T. Hepatocellular cytoplasmic changes in acute alcoholic hepatitis. JAMA. 1963 Feb 2;183:343–346. doi: 10.1001/jama.1963.63700050013013c. [DOI] [PubMed] [Google Scholar]
  28. STOECKENIUS W. An electron microscope study of myelin figures. J Biophys Biochem Cytol. 1959 May 25;5(3):491–500. doi: 10.1083/jcb.5.3.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. STOECKENIUS W. Electron microscopy of DNA molecules "stained" with heavy metal salts. J Biophys Biochem Cytol. 1961 Nov;11:297–310. doi: 10.1083/jcb.11.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. SVOBODA D., HIGGINSON J. ULTRASTRUCTURAL CHANGES PRODUCED BY PROTEIN AND RELATED DEFICIENCIES IN THE RAT LIVER. Am J Pathol. 1964 Sep;45:353–379. [PMC free article] [PubMed] [Google Scholar]
  31. Schuster F. L. A deoxyribose nucleic acid component in mitochondria of Didymium nigripes, a slime mold. Exp Cell Res. 1965 Sep;39(2):329–345. doi: 10.1016/0014-4827(65)90038-8. [DOI] [PubMed] [Google Scholar]
  32. Sorenson G. D., Heefner W. A., Kirkpatrick J. B. Experimental amyloidosis. II. Light and electron microscopic observations of liver. Am J Pathol. 1964 Apr;44(4):629–644. [PMC free article] [PubMed] [Google Scholar]
  33. Srebro Z. The ultrastructure of gliosomes in the brains of amphibia. J Cell Biol. 1965 Aug;26(2):313–322. doi: 10.1083/jcb.26.2.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Svoboda D. J., Manning R. T. Chronic alcoholism with fatty metamorphosis of the liver. Mitochondrial alterations in hepatic cells. Am J Pathol. 1964 Apr;44(4):645–662. [PMC free article] [PubMed] [Google Scholar]
  35. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. WILLS E. J. CRYSTALLINE STRUCTURES IN THE MITOCHONDRIA OF NORMAL HUMAN LIVER PARENCHYMAL CELLS. J Cell Biol. 1965 Mar;24:511–514. doi: 10.1083/jcb.24.3.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Yamamoto I. Ultrastructural changes in mesenteric lymph nodes of mice infected with Salmonella enteritidis. J Infect Dis. 1966 Feb;116(1):8–20. doi: 10.1093/infdis/116.1.8. [DOI] [PubMed] [Google Scholar]

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