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. 1967 Jul 1;34(1):293–310. doi: 10.1083/jcb.34.1.293

STUDIES OF THE MITOCHONDRIAL ENERGY-TRANSFER SYSTEM OF BROWN ADIPOSE TISSUE

O Lindberg 1, J De Pierre 1, E Rylander 1, B A Afzelius 1
PMCID: PMC2107234  PMID: 6033537

Abstract

An investigation of the mechanisms of norepinephrine action and heat production in brown adipose tissue from newborn rabbits has been carried out. Data obtained with the use of biochemical techniques has been correlated with morphological data from electron microscopy. Norepinephrine was found to stimulate the respiration of brown fat in vitro. Inhibitors of glycolysis abolish this effect, whereas inhibitors of oxidative phosphorylation do not, at least not to the same extent. Brown fat is readily permeable to added Krebs cycle intermediates. Substrate level phosphorylation, but no electron transport-coupled phosphorylation, could be demonstrated in isolated mitochondria. It is suggested that the rate of fatty acid oxidation is limited by the availability of phosphate acceptor systems which break down ATP formed at the substrate level and thus provide ADP for further substrate level phosphorylation. The theory of respiratory control by the action of reesterification of fatty acids is discussed in the light of these findings. Under the electron microscope, brown fat mitochondria are characterized by their large size, tightly packed cristae, and by the different types of granules in the matrix. No elementary particles are seen when the mitochondria are examined by the negative-staining technique. The absence of electron transport-coupled phosphorylation together with the apparent absence of elementary particles seems to be of particular significance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALL E. G., JUNGAS R. L. On the action of hormones which accelerate the rate of oxygen consumption and fatty acid release in rat adipose tissue in vitro. Proc Natl Acad Sci U S A. 1961 Jul 15;47:932–941. doi: 10.1073/pnas.47.7.932. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ball E. G. Some energy relationships in adipose tissue. Ann N Y Acad Sci. 1965 Oct 8;131(1):225–234. doi: 10.1111/j.1749-6632.1965.tb34791.x. [DOI] [PubMed] [Google Scholar]
  3. CAHILL G. F., Jr, LEBOEUF B., RENOLD A. E. Studies on rat adipose tissue in vitro. III. Synthesis of glycogen and glyceride-glycerol. J Biol Chem. 1959 Oct;234:2540–2543. [PubMed] [Google Scholar]
  4. DAWKINS M. J., HULL D. BROWN ADIPOSE TISSUE AND THE RESPONSE OF NEW-BORN RABBITS TO COLD. J Physiol. 1964 Aug;172:216–238. doi: 10.1113/jphysiol.1964.sp007414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. ERNSTER L., LOW H. Reconstruction of oxidative phosphorylation in aged mitochondrial systems. Exp Cell Res. 1955;(Suppl 3):133–153. [PubMed] [Google Scholar]
  6. FERNANDEZ MORAN H., ODA T., BLAIR P. V., GREEN D. E. A MACROMOLECULAR REPEATING UNIT OF MITOCHONDRIAL STRUCTURE AND FUNCTION. CORRELATED ELECTRON MICROSCOPIC AND BIOCHEMICAL STUDIES OF ISOLATED MITOCHONDRIA AND SUBMITOCHONDRIAL PARTICLES OF BEEF HEART MUSCLE. J Cell Biol. 1964 Jul;22:63–100. doi: 10.1083/jcb.22.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hull D., Segall M. M. Sympathetic nervous control of brown adipose tissue and heat production in the new-born rabbit. J Physiol. 1965 Dec;181(3):458–467. doi: 10.1113/jphysiol.1965.sp007774. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LEE C. P., ERNSTER L. RESTORATION OF OXIDATIVE PHOSPHORYLATION IN NON-PHOSPHORYLATING SUBMITOCHONDRIAL PARTICLES BY OLIGOMYCIN. Biochem Biophys Res Commun. 1965 Feb 17;18:523–529. doi: 10.1016/0006-291x(65)90785-0. [DOI] [PubMed] [Google Scholar]
  9. LOW H., SIEKEVITZ P., ERNSTER L., LINDBERG O. Studies on the relation of the adenosinetriphosphate-inorganic phosphate exchange reaction of mitochondria to oxidative phosphorylation. Biochim Biophys Acta. 1958 Aug;29(2):392–405. doi: 10.1016/0006-3002(58)90198-7. [DOI] [PubMed] [Google Scholar]
  10. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. OHNISHI T., HAGIHARA B. PREPARATION OF YEAST MITOCHONDRIA BY AN ENZYMATIC PROCEDURE. J Biochem. 1964 Nov;56:484–486. doi: 10.1093/oxfordjournals.jbchem.a128022. [DOI] [PubMed] [Google Scholar]
  12. Okuda H., Yanagi I., Fujii S. The mechanism of in vitro stimulation of lipolysis by adrenaline. J Biochem. 1966 May;59(5):438–442. doi: 10.1093/oxfordjournals.jbchem.a128324. [DOI] [PubMed] [Google Scholar]
  13. PEASE D. C. Demonstration of a highly ordered pattern upon a mitochondrial surface. J Cell Biol. 1962 Nov;15:385–389. doi: 10.1083/jcb.15.2.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Picheral B. Sur l'ultrastructure des mitochondries des cellules du tissu glandulaire du testicule de Pleurodeles waltlii Michah. (Amphibien Urodèle) C R Acad Sci Hebd Seances Acad Sci D. 1966 Apr 18;262(16):1769–1772. [PubMed] [Google Scholar]
  15. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. ROSSI C. R., GIBSON D. M. ACTIVATION OF FATTY ACIDS BY A GUANOSINE TRIPHOSPHATE-SPECIFIC THIOKINASE FROM LIVER MITOCHONDRIA. J Biol Chem. 1964 Jun;239:1694–1699. [PubMed] [Google Scholar]
  17. SABATINI D. D., MILLER F., BARRNETT R. J. ALDEHYDE FIXATION FOR MORPHOLOGICAL AND ENZYME HISTOCHEMICAL STUDIES WITH THE ELECTRON MICROSCOPE. J Histochem Cytochem. 1964 Feb;12:57–71. doi: 10.1177/12.2.57. [DOI] [PubMed] [Google Scholar]
  18. SIEKEVITZ P., LOW H., ERNSTER L., LINDBERG O. On a possible mechanism of the adenosinetriphosphatase of liver mitochondria. Biochim Biophys Acta. 1958 Aug;29(2):378–391. doi: 10.1016/0006-3002(58)90197-5. [DOI] [PubMed] [Google Scholar]
  19. SMITH R. E., HOCK R. J. Brown fat: thermogenic effector of arousal in hibernators. Science. 1963 Apr 12;140(3563):199–200. doi: 10.1126/science.140.3563.199. [DOI] [PubMed] [Google Scholar]
  20. Smith R. E., Roberts J. C., Hittelman K. J. Nonphosphorylating respiration of mitochondria from brown adipose tissue of rats. Science. 1966 Nov 4;154(3749):653–654. doi: 10.1126/science.154.3749.653. [DOI] [PubMed] [Google Scholar]
  21. VAN DEN BERGH S. G. TWO SYSTEMS FOR THE ACTIVATION OF FATTY ACIDS IN RAT-LIVER MITOCHONDRIA. Biochim Biophys Acta. 1965 Apr 5;98:442–444. doi: 10.1016/0005-2760(65)90139-6. [DOI] [PubMed] [Google Scholar]

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