Abstract
RNA was analyzed in the whole rod cell and in the rod cell nucleus of the rabbit retina. The analysis was performed on rod cells or rod cell nuclei after they were isolated by microdissection and collected. The nuclei were denuded by selectively lysing inner and outer segments chemically. The rod cell contained an average of 0.65 µµg RNA. About 60% of the total RNA was nuclear. RNA concentration was of the order of 0.4% w/v. RNA base composition was determined for the whole rod cell and for the rod cell nucleus, and from it the base composition of cytoplasmic RNA was derived also. Microdissection of the retina revealed that each Müller cell had firmly attached to it a certain complement of visual cells, forming a bouquet-like arrangement. The unusual susceptibility of the inner and outer segments to lysis was regarded as an indication of an unique composition of the plasma membrane.
Full Text
The Full Text of this article is available as a PDF (823.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DE ROBERTIS E. Electron microscope observations on the submicroscopic organization of the retinal rods. J Biophys Biochem Cytol. 1956 May 25;2(3):319–330. doi: 10.1083/jcb.2.3.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DOWLING J. E., SIDMAN R. L. Inherited retinal dystrophy in the rat. J Cell Biol. 1962 Jul;14:73–109. doi: 10.1083/jcb.14.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EDSTROM J. E., EICHNER D. Quantitative Untersuchungen über den Ribonukleinsäuregehalt der Netzhautganglienzellen bei Rind und mensch. Z Mikrosk Anat Forsch. 1957;63(3):413–421. [PubMed] [Google Scholar]
- EDSTROM J. E., KAWIAK J. Microchemical deoxyribonucleic acid determination in individual cells. J Biophys Biochem Cytol. 1961 Mar;9:619–626. doi: 10.1083/jcb.9.3.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EHRLICH G., DISCHE Z. Content of desoxyribo and ribonucleic acids of retina under various conditions. Proc Soc Exp Biol Med. 1950 May;74(1):40–42. doi: 10.3181/00379727-74-17802. [DOI] [PubMed] [Google Scholar]
- Grignolo A., Orzalesi N., Calabria G. A. Studies on the fine structure and the rhodopsin cycle of the rabbit retina in experimental degeneration induced by sodium iodate. Exp Eye Res. 1966 Jan;5(1):86–97. doi: 10.1016/s0014-4835(66)80024-6. [DOI] [PubMed] [Google Scholar]
- HYDEN H. Quantitative assay of compounds in isolated, fresh nerve cells and glial cells from control and stimulated animals. Nature. 1959 Aug 8;184:433–435. doi: 10.1038/184433a0. [DOI] [PubMed] [Google Scholar]
- KOENIG E. SYNTHETIC MECHANISMS IN THE AXON. II. RNA IN MYELIN-FREE AXONS OF THE CAT. J Neurochem. 1965 May;12:357–361. doi: 10.1111/j.1471-4159.1965.tb04236.x. [DOI] [PubMed] [Google Scholar]
- NOELL W. K. The impairment of visual cell structure by iodoacetate. J Cell Physiol. 1952 Aug;40(1):25–55. doi: 10.1002/jcp.1030400104. [DOI] [PubMed] [Google Scholar]