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. 1988 Feb;170(2):817–820. doi: 10.1128/jb.170.2.817-820.1988

Amino acid transport by membrane vesicles of an obligate anaerobic bacterium, Clostridium acetobutylicum.

A J Driessen 1, T Ubbink-Kok 1, W N Konings 1
PMCID: PMC210727  PMID: 2828326

Abstract

Membrane vesicles were isolated from the obligate anaerobic bacterium Clostridium acetobutylicum. Beef heart mitochondrial cytochrome c oxidase was inserted in these membrane vesicles by membrane fusion by using the freeze-thaw sonication technique (A. J. M. Driessen, W. de Vrij, and W. N. Konings, Proc. Natl. Acad. Sci. USA 82:7555-7559, 1985) to accommodate them with a functional proton motive force-generating system. With ascorbate-N,N,N',N'-tetramethyl-p-phenylenediamine-cytochrome c as the electron donor, a proton motive force (delta p) of -80 to -120 mV was generated in these fused membranes. This delta p drove the accumulation of leucine and lysine up to 40- and 100-fold, respectively. High transport activities were observed in fused membranes containing Escherichia coli lipids, whereas the transport activities in fused membranes containing mainly soybean lipids or phosphatidylcholine were low. It is suggested that branched-chain amino acids and lysine were taken up by separate systems. The effects of the ionophores nigericin and valinomycin indicated that lysine and leucine were translocated in symport with a proton.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMES B. N., DUBIN D. T. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960 Mar;235:769–775. [PubMed] [Google Scholar]
  2. Barker H. A. Amino acid degradation by anaerobic bacteria. Annu Rev Biochem. 1981;50:23–40. doi: 10.1146/annurev.bi.50.070181.000323. [DOI] [PubMed] [Google Scholar]
  3. Booth I. R., Morris J. G. Carbohydrate transport in Clostridium pasteurianum. Biosci Rep. 1982 Jan;2(1):47–53. doi: 10.1007/BF01142198. [DOI] [PubMed] [Google Scholar]
  4. Driessen A. J., Hellingwerf K. J., Konings W. N. Mechanism of energy coupling to entry and exit of neutral and branched chain amino acids in membrane vesicles of Streptococcus cremoris. J Biol Chem. 1987 Sep 15;262(26):12438–12443. [PubMed] [Google Scholar]
  5. Driessen A. J., Hellingwerf K. J., Konings W. N. Membrane systems in which foreign proton pumps are incorporated. Microbiol Sci. 1987 Jun;4(6):173–180. [PubMed] [Google Scholar]
  6. Driessen A. J., Kodde J., de Jong S., Konings W. N. Neutral amino acid transport by membrane vesicles of Streptococcus cremoris is subject to regulation by internal pH. J Bacteriol. 1987 Jun;169(6):2748–2754. doi: 10.1128/jb.169.6.2748-2754.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Driessen A. J., Poolman B., Kiewiet R., Konings W. Arginine transport in Streptococcus lactis is catalyzed by a cationic exchanger. Proc Natl Acad Sci U S A. 1987 Sep;84(17):6093–6097. doi: 10.1073/pnas.84.17.6093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Driessen A. J., de Jong S., Konings W. N. Transport of branched-chain amino acids in membrane vesicles of Streptococcus cremoris. J Bacteriol. 1987 Nov;169(11):5193–5200. doi: 10.1128/jb.169.11.5193-5200.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Driessen A. J., de Vrij W., Konings W. N. Functional incorporation of beef-heart cytochrome c oxidase into membranes of Streptococcus cremoris. Eur J Biochem. 1986 Feb 3;154(3):617–624. doi: 10.1111/j.1432-1033.1986.tb09443.x. [DOI] [PubMed] [Google Scholar]
  10. Driessen A. J., de Vrij W., Konings W. N. Incorporation of beef heart cytochrome c oxidase as a proton-motive force-generating mechanism in bacterial membrane vesicles. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7555–7559. doi: 10.1073/pnas.82.22.7555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Johnston N. C., Goldfine H. Lipid composition in the classification of the butyric acid-producing clostridia. J Gen Microbiol. 1983 Apr;129(4):1075–1081. doi: 10.1099/00221287-129-4-1075. [DOI] [PubMed] [Google Scholar]
  12. Kaback H. R. Active transport in Escherichia coli: passage to permease. Annu Rev Biophys Biophys Chem. 1986;15:279–319. doi: 10.1146/annurev.bb.15.060186.001431. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. NISMAN B. The Stickland reaction. Bacteriol Rev. 1954 Mar;18(1):16–42. doi: 10.1128/br.18.1.16-42.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Otto R., Lageveen R. G., Veldkamp H., Konings W. N. Lactate efflux-induced electrical potential in membrane vesicles of Streptococcus cremoris. J Bacteriol. 1982 Feb;149(2):733–738. doi: 10.1128/jb.149.2.733-738.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shinbo T., Kamo N., Kurihara K., Kobatake Y. A PVC-based electrode sensitive to DDA+ as a device for monitoring the membrane potential in biological systems. Arch Biochem Biophys. 1978 Apr 30;187(2):414–422. doi: 10.1016/0003-9861(78)90052-8. [DOI] [PubMed] [Google Scholar]
  17. Yu C., Yu L., King T. E. Studies on cytochrome oxidase. Interactions of the cytochrome oxidase protein with phospholipids and cytochrome c. J Biol Chem. 1975 Feb 25;250(4):1383–1392. [PubMed] [Google Scholar]
  18. Zeikus J. G. Chemical and fuel production by anaerobic bacteria. Annu Rev Microbiol. 1980;34:423–464. doi: 10.1146/annurev.mi.34.100180.002231. [DOI] [PubMed] [Google Scholar]
  19. de Vrij W., Driessen A. J., Hellingwerf K. J., Konings W. N. Measurements of the proton motive force generated by cytochrome c oxidase from Bacillus subtilis in proteoliposomes and membrane vesicles. Eur J Biochem. 1986 Apr 15;156(2):431–440. doi: 10.1111/j.1432-1033.1986.tb09600.x. [DOI] [PubMed] [Google Scholar]

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