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. 1967 Aug 1;34(2):647–675. doi: 10.1083/jcb.34.2.647

STUDIES ON THE HUMAN OOCYTE AND ITS FOLLICLE

I. Ultrastructural and Histochemical Observations on the Primordial Follicle Stage

Arthur T Hertig 1, Eleanor C Adams 1
PMCID: PMC2107315  PMID: 4292010

Abstract

Oocytes in primordial ("resting") follicles in adult human ovaries contain a complex paranuclear structure identified by light microscopists as Balbiani's vitelline body. By electron microscopy this structure is composed of a mass of mitochondria with associated endoplasmic reticulum, multiple compound aggregates which form a ring around the cytocentrum, and a single stack or coil of annulate lamellae either attached to the nuclear membrane or free in the cytoplasm. The compound aggregates contain vacuoles and finely divided electron-opaque material. Evidence is presented for the probable transport of this material between the oocyte and its environment. The cytocentrum contains a central aggregate of amorphous electron-opaque deposits which appear to become periodically aligned on fine fibrils to form the long coarse fibers at the periphery of the cytocentrum. The apparent prevalence of annulate lamellae attached or adjacent to the nuclear membrane of oocytes in ovaries removed during the mid-follicular (estrogenic) phase of the cycle indicates the need for further study of a possible hormonal influence on the resting oocyte. By light microscopy phosphatases were not found within the oocyte, but adenosine-monophosphatase activity is present in the cortical cells surrounding primordial follicles, and also at the periphery of each primitive follicle cell, most prominently at the oocyte side. Glucose-6-phosphate dehydrogenase activity is present within the oocyte cytoplasm.

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Selected References

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  1. ADAMS E. C., HERTIG A. T. STUDIES ON GUINEA PIG OOCYTES. I. ELECTRON MICROSCOPIC OBSERVATIONS ON THE DEVELOPMENT OF CYTOPLASMIC ORGANELLES IN OOCYTES OF PRIMORDIAL AND PRIMARY FOLLICLES. J Cell Biol. 1964 Jun;21:397–427. doi: 10.1083/jcb.21.3.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ANDERSON E., BEAMS H. W. Cytological observations on the fine structure of the guinea pig ovary with special reference to the oogonium, primary oocyte and associated follicle cells. J Ultrastruct Res. 1960 Jun;3:432–446. doi: 10.1016/s0022-5320(60)90021-6. [DOI] [PubMed] [Google Scholar]
  3. Adams E. C., Hertig A. T., Foster S. Studies on guinea pig oocytes. II. Histochemical observations on some phosphatases and lipid in developing and in atretic oocytes and follicles. Am J Anat. 1966 Sep;119(2):303–339. doi: 10.1002/aja.1001190207. [DOI] [PubMed] [Google Scholar]
  4. BAKER T. G. A QUANTITATIVE AND CYTOLOGICAL STUDY OF GERM CELLS IN HUMAN OVARIES. Proc R Soc Lond B Biol Sci. 1963 Oct 22;158:417–433. doi: 10.1098/rspb.1963.0055. [DOI] [PubMed] [Google Scholar]
  5. BECKER N. H., GOLDFISCHER S., SHIN W. Y., NOVIKOFF A. B. The localization of enzyme activities in the rat brain. J Biophys Biochem Cytol. 1960 Dec;8:649–663. doi: 10.1083/jcb.8.3.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fahimi H. D., Karnovsky M. J. Cytochemical localization of two glycolytic dehydrogenases in white skeletal muscle. J Cell Biol. 1966 Apr;29(1):113–128. doi: 10.1083/jcb.29.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Greenfield M. L. The oocyte of the domestic chicken shortly after hatching, studied by electron microscopy. J Embryol Exp Morphol. 1966 Jun;15(3):297–316. [PubMed] [Google Scholar]
  8. Hope J. The fine structure of the developing follicle of the rhesus ovary. J Ultrastruct Res. 1965 Jun;12(5):592–610. doi: 10.1016/s0022-5320(65)80050-8. [DOI] [PubMed] [Google Scholar]
  9. KARNOVSKY M. J., HIMMELHOCH S. R. The use of ethylene diamine tetraacetic acid disodium in the histochemical demonstration of triphosphopyridine nucleotide-linked dehydrogenases. J Histochem Cytochem. 1961 Mar;9:203–204. doi: 10.1177/9.2.203. [DOI] [PubMed] [Google Scholar]
  10. KESSEL R. G. ELECTRON MICROSCOPE STUDIES ON OOCYTES AND ECHINODERM, THYONE BRIAREUS, WITH SPECIAL REFERENCE TO THE ORIGIN AND STRUCTURE OF THE ANNULATE LAMELLAE. J Ultrastruct Res. 1964 Jun;10:498–514. doi: 10.1016/s0022-5320(64)80025-3. [DOI] [PubMed] [Google Scholar]
  11. KESSEL R. G. ELECTRON MICROSCOPE STUDIES ON THE ORIGIN OF ANNULATE LAMELLAE IN OOCYTES OF NECTURUS. J Cell Biol. 1963 Nov;19:391–414. doi: 10.1083/jcb.19.2.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kessel R. G. Some observations on the ultrastructure of the oocyte of Thyone briareus with special reference to the relationship of the Golgi complex and endoplasmic reticulum in the formation of yolk. J Ultrastruct Res. 1966 Oct;16(3):305–319. doi: 10.1016/s0022-5320(66)80065-5. [DOI] [PubMed] [Google Scholar]
  13. Merriam R. W. The role of cytoplasmic membranes in the regulation of water concentration within frog oocytes. Exp Cell Res. 1966 Jan;41(1):34–48. doi: 10.1016/0014-4827(66)90544-1. [DOI] [PubMed] [Google Scholar]
  14. ODOR D. L. THE ULTRASTRUCTURE OF UNILAMINAR FOLLICLES OF THE HAMSTER OVARY. Am J Anat. 1965 May;116:493–521. doi: 10.1002/aja.1001160304. [DOI] [PubMed] [Google Scholar]
  15. SOTELO J. R. An electron microscope study on the cytoplasmic and nuclear components of rat primary oocytes. Z Zellforsch Mikrosk Anat. 1959;50:749–765. doi: 10.1007/BF00342364. [DOI] [PubMed] [Google Scholar]
  16. SWIFT H. The fine structure of annulate lamellae. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):415–418. doi: 10.1083/jcb.2.4.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. TARDINI A., VITALI-MAZZA L., MANSANI F. E. [Ultrastructure of the mature human ovocyte. I. Relation between cells of the corona radiata, zona pellucida and ovoplasm]. Arch De Vecchi Anat Patol. 1960 Sep;33:281–305. [PubMed] [Google Scholar]
  18. VENABLE J. H., COGGESHALL R. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY. J Cell Biol. 1965 May;25:407–408. doi: 10.1083/jcb.25.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WACHSTEIN M., MEISEL E. Histochemistry of hepatic phosphatases of a physiologic pH; with special reference to the demonstration of bile canaliculi. Am J Clin Pathol. 1957 Jan;27(1):13–23. doi: 10.1093/ajcp/27.1.13. [DOI] [PubMed] [Google Scholar]
  20. WARTENBERG H., STEGNER H. E. [On the electron microscopic fine structure of the human ovarian egg]. Z Zellforsch Mikrosk Anat. 1960;52:450–474. [PubMed] [Google Scholar]
  21. Weakley B. S. Electron microscopy of the oocyte and granulosa cells in the developing ovarian follicles of the golden hamster (Mesocricetus auratus). J Anat. 1966 Jul;100(Pt 3):503–534. [PMC free article] [PubMed] [Google Scholar]
  22. Zamboni L., Mishell D. R., Jr, Bell J. H., Baca M. Fine structure of the human ovum in the pronuclear stage. J Cell Biol. 1966 Sep;30(3):579–600. doi: 10.1083/jcb.30.3.579. [DOI] [PMC free article] [PubMed] [Google Scholar]

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