Abstract
A reproducible method for the preparation of 75S ribosomes from cysts of Entamoeba invadens is presented. The method depends on the inactivation of the cyst's ribonuclease by high levels of Bentonite. The ribosomes are found to be extremely sensitive to ribonuclease, and to be stabilized by the addition of manganous and calcium ions to the magnesium customarily employed. Reasons are given for equating these ribosomes with the particles of which the crystalline chromatoid bodies are made.
Full Text
The Full Text of this article is available as a PDF (1.9 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BALAMUTH W. Effects of some environmental factors upon growth and encystation of Entamoeba invadens. J Parasitol. 1962 Feb;48:101–109. [PubMed] [Google Scholar]
- BARKER D. C. A ribonucleoprotein inclusion body in Entamoeba invadens. Z Zellforsch Mikrosk Anat. 1963;58:641–659. doi: 10.1007/BF00410654. [DOI] [PubMed] [Google Scholar]
- BARKER D. C. RIBOSOME STRUCTURES REVEALED BY NEGATIVE STAINING SUBCELLULAR FRACTIONS FROM A CRYSTALLINE RIBONUCLEOPROTEIN BODY. Exp Cell Res. 1963 Nov;32:272–279. doi: 10.1016/0014-4827(63)90102-2. [DOI] [PubMed] [Google Scholar]
- BARKER D. C., SVIHLA G. LOCALIZATION OF CYTOPLASMIC NUCLEIC ACID DURING GROWTH AND ENCYSTMENT OF ENTAMOEBA INVADENS. J Cell Biol. 1964 Mar;20:389–398. doi: 10.1083/jcb.20.3.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BROWNHILL T. J., JONES A. S., STACEY M. The inactivation of ribonuclease during the isolation of ribonucleic acids and ribonucleoproteins from yeast. Biochem J. 1959 Nov;73:434–438. doi: 10.1042/bj0730434. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CABLE R. M. "THEREBY HANGS A TAIL". J Parasitol. 1965 Feb;51:3–12. [PubMed] [Google Scholar]
- Carter W. A., Levy H. B., Diamond L. S. Protein synthesis by amoebal ribosomes. Nature. 1967 Feb 18;213(5077):722–724. doi: 10.1038/213722b0. [DOI] [PubMed] [Google Scholar]
- DROCHMANS P. [Morphology of glycogen. Electron microscopic study of the negative stains of particulate glycogen]. J Ultrastruct Res. 1962 Apr;6:141–163. doi: 10.1016/s0022-5320(62)90050-3. [DOI] [PubMed] [Google Scholar]
- LYTTLETON J. W. Stabilization by manganous ions of ribosomes from embryonic plant tissue. Nature. 1960 Sep 17;187:1026–1027. doi: 10.1038/1871026a0. [DOI] [PubMed] [Google Scholar]
- Morgan R. S., Uzman B. G. Nature of the packing of ribosomes within chromatoid bodies. Science. 1966 Apr 8;152(3719):214–216. doi: 10.1126/science.152.3719.214. [DOI] [PubMed] [Google Scholar]
- REVEL J. P. ELECTRON MICROSCOPY OF GLYCOGEN. J Histochem Cytochem. 1964 Feb;12:104–114. doi: 10.1177/12.2.104. [DOI] [PubMed] [Google Scholar]
- SLAYTER H. S., WARNER J. R., RICH A., HALL C. E. THE VISUALIZATION OF POLYRIBOSOMAL STRUCTURE. J Mol Biol. 1963 Dec;7:652–657. doi: 10.1016/s0022-2836(63)80112-6. [DOI] [PubMed] [Google Scholar]