Abstract
The cytological changes observed in the A and D cells of rabbit endocrine pancreas incubated in a medium containing 0.6 mg/ml or 3 mg/ml of glucose are described. These cells showed no changes in their fine structure nor any signs of degranulation. When the A cells were incubated in a medium without glucose, they released A granules and synthesized new hormone. The way in which A granules are eliminated is compared to that following insulinic hypoglycemia in the animal in vivo. In both cases, the mechanism of secretion involves margination, emiocytosis of the entire granule, and formation of microvilli, in contrast to previously reported observations (9). The D cells showed no alteration of their fine structure after incubation with different concentrations of glucose in the medium. Only very rarely could we observe morphological changes which were suggestive of emiocytosis of the entire D granule.
Full Text
The Full Text of this article is available as a PDF (1.8 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- GOMORI G. A rapid one-step trichrome stain. Am J Clin Pathol. 1950 Jul;20(7):661–664. doi: 10.1093/ajcp/20.7_ts.661. [DOI] [PubMed] [Google Scholar]
- Gomez-Acebo J., Lopez-Quijada C., R-Candela J. L. Fine structure of B-cell from pancreas pieces incubated in vitro. Diabetologia. 1966 Sep;2(2):110–116. doi: 10.1007/BF00423019. [DOI] [PubMed] [Google Scholar]
- LACY P. E. Electron microscopic identification of different cell types in the islets of Langerhans of the guinea pig, rat, rabbit and dog. Anat Rec. 1957 Jun;128(2):255–267. doi: 10.1002/ar.1091280209. [DOI] [PubMed] [Google Scholar]
- LACY P. E. Electron microscopy of the normal islets of Langerhans; studies in the dog, rabbit, guinea pig and rat. Diabetes. 1957 Nov-Dec;6(6):498–507. doi: 10.2337/diab.6.6.498. [DOI] [PubMed] [Google Scholar]
- LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lopez-Quijada C., Gomez-Acebo J., Candela J. L. Decrease in the insulin of rabbit pancreas in late pregnancy. Diabetologia. 1967 Oct;3(5):435–442. doi: 10.1007/BF01228079. [DOI] [PubMed] [Google Scholar]
- Meyer J., Bencosme S. A. The fine structure of normal rabbit pancreatic islet cells. Rev Can Biol. 1965 Sep;24(3):179–205. [PubMed] [Google Scholar]
- Munger B. L., Caramia F., Lacy P. E. The ultrastructural basis for the identification of cell types in the pancreatic islets. II. Rabbit, dog and opossum. Z Zellforsch Mikrosk Anat. 1965 Sep 17;67(6):776–798. doi: 10.1007/BF00339301. [DOI] [PubMed] [Google Scholar]
- PAGET G. E. Aldehyde-thionin: a stain having similar properties to aldehydefuchsin. Stain Technol. 1959 Jul;34(4):223–226. doi: 10.3109/10520295909114679. [DOI] [PubMed] [Google Scholar]
- REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WILLIAMSON J. R., LACY P. E., GRISHAM J. W. Ultrastructural changes in islets of the rat produced by tolbutamide. Diabetes. 1961 Nov-Dec;10:460–469. doi: 10.2337/diab.10.6.460. [DOI] [PubMed] [Google Scholar]