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. 1968 May 1;37(2):445–461. doi: 10.1083/jcb.37.2.445

THE FINE STRUCTURE OF THE VENTRAL INTERSEGMENTAL ABDOMINAL MUSCLES OF THE INSECT RHODNIUS PROLIXUS DURING THE MOLTING CYCLE

I. Muscle Structure at Molting

Paul A Toselli 1, Frank A Pepe 1
PMCID: PMC2107409  PMID: 5656401

Abstract

Rhodnius prolixus, a South American insect, molts five times in its development to an adult after emerging from the egg. Each molting cycle is triggered with a blood-meal. The ventral intersegmental abdominal muscles of Rhodnius develop during each molting cycle and are functional at molting. The fine structure of these fully developed muscles from fourth stage larval insects is studied. They have the characteristic structure of slow muscles. They have multiple motor nerve endings, and the myofibrils are poorly defined in cross-section. Longitudinal sections show long sarcomeres (8–10 µ), irregular Z-lines, and no apparent H zones. No M line is seen. Transverse sections through the A-band region show that each hexagonally arranged thick filament is surrounded by 12 thin filaments. Two thin filaments are shared by two neighboring thick filaments. The ratio of thin to thick filaments is 6:1. This structure is related to that found in vertebrate skeletal muscle and insect flight muscle.

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Selected References

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  1. ABD-EL-WAHAB A. A new method for delaying pupation in Drosophila melanogaster. Nature. 1959 Jan 10;183(4654):127–127. doi: 10.1038/183127a0. [DOI] [PubMed] [Google Scholar]
  2. Brandt P. W., Reuben J. P., Girardier L., Grundfest H. Correlated morphological and physiological studies on isolated single muscle fibers. I. Fine structure of the crayfish muscle fiber. J Cell Biol. 1965 Jun;25(3 Suppl):233–260. doi: 10.1083/jcb.25.3.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cheng K., Breinin G. M. Fine structure of nerve endings in extraocular muscle. Arch Ophthalmol. 1965 Dec;74(6):822–834. doi: 10.1001/archopht.1965.00970040824019. [DOI] [PubMed] [Google Scholar]
  4. FRANZINI-ARMSTRONG C., PORTER K. R. SARCOLEMMAL INVAGINATIONS CONSTITUTING THE T SYSTEM IN FISH MUSCLE FIBERS. J Cell Biol. 1964 Sep;22:675–696. doi: 10.1083/jcb.22.3.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fahrenbach W. H. The fine structure of fast and slow crustacean muscles. J Cell Biol. 1967 Oct;35(1):69–79. doi: 10.1083/jcb.35.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Graziadei P. The ultrastructure of the motor nerve endings in the muscles of cephalopods. J Ultrastruct Res. 1966 Apr;15(1):1–13. doi: 10.1016/s0022-5320(66)80090-4. [DOI] [PubMed] [Google Scholar]
  7. HESS A. Further morphological observations of 'en plaque' and 'en grappe' nerve endings on mammalian extrafusal muscle fibers with the cholinesterase technique. Rev Can Biol. 1962 Sep-Dec;21:241–248. [PubMed] [Google Scholar]
  8. HUXLEY A. F. Local activation of muscle. Ann N Y Acad Sci. 1959 Aug 28;81:446–452. doi: 10.1111/j.1749-6632.1959.tb49326.x. [DOI] [PubMed] [Google Scholar]
  9. HUXLEY A. F., TAYLOR R. E. Local activation of striated muscle fibres. J Physiol. 1958 Dec 30;144(3):426–441. doi: 10.1113/jphysiol.1958.sp006111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HUXLEY H. E. EVIDENCE FOR CONTINUITY BETWEEN THE CENTRAL ELEMENTS OF THE TRIADS AND EXTRACELLULAR SPACE IN FROG SARTORIUS MUSCLE. Nature. 1964 Jun 13;202:1067–1071. doi: 10.1038/2021067b0. [DOI] [PubMed] [Google Scholar]
  11. Hagopian M. The myofilament arrangement in the femoral muscle of the cockroach, Leucophaea maderae fabricius. J Cell Biol. 1966 Mar;28(3):545–562. doi: 10.1083/jcb.28.3.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hama K. The fine structure of the Schwann cell sheath of the nerve fiber in the shrimp (Penaeus japonicus). J Cell Biol. 1966 Dec;31(3):624–632. doi: 10.1083/jcb.31.3.624. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Morita M. Electron microscopic studies on Planaria. I. Fine structure of muscle fiber in the head of the planarian Dugesia dorotocephala. J Ultrastruct Res. 1965 Dec;13(5):383–395. doi: 10.1016/s0022-5320(65)90002-x. [DOI] [PubMed] [Google Scholar]
  14. PEACHEY L. D., PORTER K. R. Intracellular impulse conduction in muscle cells. Science. 1959 Mar 13;129(3350):721–722. doi: 10.1126/science.129.3350.721. [DOI] [PubMed] [Google Scholar]
  15. Peachey L. D. The sarcoplasmic reticulum and transverse tubules of the frog's sartorius. J Cell Biol. 1965 Jun;25(3 Suppl):209–231. doi: 10.1083/jcb.25.3.209. [DOI] [PubMed] [Google Scholar]
  16. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rosenbluth J. Ultrastructural organization of obliquely striated muscle fibers in Ascaris lumbricoides. J Cell Biol. 1965 Jun;25(3):495–515. doi: 10.1083/jcb.25.3.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Smith D. S., Gupta B. L., Smith U. The organization and myofilament array of insect visceral muscles. J Cell Sci. 1966 Mar;1(1):49–57. doi: 10.1242/jcs.1.1.49. [DOI] [PubMed] [Google Scholar]
  19. Smith D. S. The structure of intersegmental muscle fibers in an insect, Periplaneta americana L. J Cell Biol. 1966 Jun;29(3):449–459. doi: 10.1083/jcb.29.3.449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Toselli P. A., Pepe F. A. The fine structure of the ventral intersegmental abdominal muscles of the insect Rhodnius prolixus during the molting cycle. II. Muscle changes in preparation for molting. J Cell Biol. 1968 May;37(2):462–481. doi: 10.1083/jcb.37.2.462. [DOI] [PMC free article] [PubMed] [Google Scholar]

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