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. 1968 Jun 1;37(3):633–649. doi: 10.1083/jcb.37.3.633

STRUCTURAL MODULATIONS OF PLASMALEMMAL VESICLES

G E Palade 1, R R Bruns 1
PMCID: PMC2107438  PMID: 11905197

Abstract

Structural modulations affecting a small fraction of the population of plasmalemmal vesicles of vascular endothelia are described. They include forms which are apparently produced by the fusion of the vesicular membrane with the plasmalemma and by the successive elimination of the layers of the two fused membranes. Such modulations are assumed to represent stages in the discharge process of vesicular contents. Other forms, characterized by their flask shape and elongated neck, are assumed to represent stages in the formation and loading of membrane invaginations, followed by their being pinched off to form isolated vesicles. Stages in a membrane-fusion process leading to the formation of apertured fenestrae and channels are also described in fenestrated endothelia. The visualization of these structural details is greatly facilitated by staining tissue specimens with uranyl acetate before dehydration.

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Selected References

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  1. BENNETT H. S., LUFT J. H., HAMPTON J. C. Morphological classifications of vertebrate blood capillaries. Am J Physiol. 1959 Feb;196(2):381–390. doi: 10.1152/ajplegacy.1959.196.2.381. [DOI] [PubMed] [Google Scholar]
  2. Bainton D. F., Farquhar M. G. Origin of granules in polymorphonuclear leukocytes. Two types derived from opposite faces of the Golgi complex in developing granulocytes. J Cell Biol. 1966 Feb;28(2):277–301. doi: 10.1083/jcb.28.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bruns R. R., Palade G. E. Studies on blood capillaries. I. General organization of blood capillaries in muscle. J Cell Biol. 1968 May;37(2):244–276. doi: 10.1083/jcb.37.2.244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bruns R. R., Palade G. E. Studies on blood capillaries. II. Transport of ferritin molecules across the wall of muscle capillaries. J Cell Biol. 1968 May;37(2):277–299. doi: 10.1083/jcb.37.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DAVSON H. Growth of the concept of the paucimolecular membrane. Circulation. 1962 Nov;26:1022–1037. doi: 10.1161/01.cir.26.5.1022. [DOI] [PubMed] [Google Scholar]
  6. EKHOLM R., SJOSTRAND F. S. The ultrastructural organization of the mouse thyroid gland. J Ultrastruct Res. 1957 Dec;1(2):178–199. doi: 10.1016/s0022-5320(57)80006-9. [DOI] [PubMed] [Google Scholar]
  7. Elfvin L. G. The ultrastructure of the capillary fenestrae in the adrenal medulla of the rat. J Ultrastruct Res. 1965 Jun;12(5):687–704. doi: 10.1016/s0022-5320(65)80056-9. [DOI] [PubMed] [Google Scholar]
  8. FARQUHAR M. G. Fine structure and function in capillaries of the anterior pituitary gland. Angiology. 1961 Jul;12:270–292. doi: 10.1177/000331976101200704. [DOI] [PubMed] [Google Scholar]
  9. Farquhar M. G., Palade G. E. Cell junctions in amphibian skin. J Cell Biol. 1965 Jul;26(1):263–291. doi: 10.1083/jcb.26.1.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KARRER H. E. The striated musculature of blood vessels. II. Cell interconnections and cell surface. J Biophys Biochem Cytol. 1960 Sep;8:135–150. doi: 10.1083/jcb.8.1.135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KELLENBERGER E., RYTER A., SECHAUD J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol. 1958 Nov 25;4(6):671–678. doi: 10.1083/jcb.4.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LUCK D. J. THE INFLUENCE OF PRECURSOR POOL SIZE ON MITOCHONDRIAL COMPOSITION IN NEUROSPORA CRASSA. J Cell Biol. 1965 Mar;24:445–460. doi: 10.1083/jcb.24.3.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Luft J. H. Fine structures of capillary and endocapillary layer as revealed by ruthenium red. Fed Proc. 1966 Nov-Dec;25(6):1773–1783. [PubMed] [Google Scholar]
  15. PEACHEY L. D. Thin sections. I. A study of section thickness and physical distortion produced during microtomy. J Biophys Biochem Cytol. 1958 May 25;4(3):233–242. doi: 10.1083/jcb.4.3.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. RHODIN J. A. The diaphragm of capillary endothelial fenestrations. J Ultrastruct Res. 1962 Apr;6:171–185. doi: 10.1016/s0022-5320(62)90052-7. [DOI] [PubMed] [Google Scholar]
  18. ROBERTSON J. D. Ultrastructure of excitable membranes and the crayfish median-giant synapse. Ann N Y Acad Sci. 1961 Sep 6;94:339–389. doi: 10.1111/j.1749-6632.1961.tb35552.x. [DOI] [PubMed] [Google Scholar]
  19. STEMPAK J. G., WARD R. T. AN IMPROVED STAINING METHOD FOR ELECTRON MICROSCOPY. J Cell Biol. 1964 Sep;22:697–701. doi: 10.1083/jcb.22.3.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. VENABLE J. H., COGGESHALL R. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY. J Cell Biol. 1965 May;25:407–408. doi: 10.1083/jcb.25.2.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]

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