Abstract
The kinetoplastic DNA of Trypanosoma lewisi is described as a filamentous body lying within a mitochondrion, with the filaments oriented parallel to the long axis of the cell. The manner of fixation, the replicative state, and perhaps the physiological state of the cell, may result in slight morphological differences among such bodies. The kinetoplastic DNA replicates to form "left" and "right" rather than "upper" and "lower" members, and both the kinetoplast and nucleus incorporate radiothymidine as shown by radioautography. Radioautographic analyses suggest a random incorporation of radiothymidine by kinetoplasts. Silver grains were occasionally observed over centriolar elements. Finally, the observations are discussed with respect to the sequential replication of the aforementioned organelles by T. lewisi.
Full Text
The Full Text of this article is available as a PDF (1.9 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CARO L. G., VAN TUBERGEN R. P., KOLB J. A. High-resolution autoradiography. I. Methods. J Cell Biol. 1962 Nov;15:173–188. doi: 10.1083/jcb.15.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chang H. T., Ruch T. C. Topographical distribution of spinothalamic fibres in the thalamus of the spider monkey. J Anat. 1947 Apr;81(Pt 2):150–164.2. [PMC free article] [PubMed] [Google Scholar]
- Du Buy H. G., Mattern C. F., Riley F. L. Comparison of the DNA's obtained from brain nuclei and mitochondria of mice and from the nuclei and kinetoplasts of Leishmania enriettii. Biochim Biophys Acta. 1966 Aug 17;123(2):298–305. doi: 10.1016/0005-2787(66)90282-6. [DOI] [PubMed] [Google Scholar]
- Dusanic D. G. Growth and immunologic studies on the culture forms of Trypanosoma lewisi. J Protozool. 1968 May;15(2):328–333. doi: 10.1111/j.1550-7408.1968.tb02131.x. [DOI] [PubMed] [Google Scholar]
- JUDGE D. M., ANDERSON M. S. ULTRASTRUCTURE OF TRYPANOSOMA LEWISI. J Parasitol. 1964 Dec;50:757–762. [PubMed] [Google Scholar]
- Riou G., Paoletti C. Preparation and properties of nuclear and satellite deoxyribonucleic acid of Trypanosoma cruzi. J Mol Biol. 1967 Sep 14;28(2):377–382. doi: 10.1016/s0022-2836(67)80017-2. [DOI] [PubMed] [Google Scholar]
- Riou G., Pautrizel R., Paoletti C. Fractionnement et caractérisation de l'acide désoxyribonucléique (DNA) de trypanosome (Trypanosoma equiperdum) C R Acad Sci Hebd Seances Acad Sci D. 1966 Jun 1;262(22):2376–2379. [PubMed] [Google Scholar]
- SCHULZ H., MACCLURE E. [Electron microscopic study of Trypanosoma cruzi with special reference to periplasts and blepharoplasts]. Z Zellforsch Mikrosk Anat. 1961;55:389–412. [PubMed] [Google Scholar]
- STEINERT G., FIRKET H., STEINERT M. Synthèse d'acide désoxyribonucléique dans le corps parabasal de Trypanosoma mega. Exp Cell Res. 1958 Dec;15(3):632–635. doi: 10.1016/0014-4827(58)90117-4. [DOI] [PubMed] [Google Scholar]
- STEINERT M., STEINERT G. [The synthesis of desoxyri-bonucleic acid during the division cycle of Trypanosoma mega]. J Protozool. 1962 May;9:203–211. doi: 10.1111/j.1550-7408.1962.tb02607.x. [DOI] [PubMed] [Google Scholar]
- Smith-Sonneborn J., Plaut W. Evidence for the presence of DNA in the pellicle of Paramecium. J Cell Sci. 1967 Jun;2(2):225–234. doi: 10.1242/jcs.2.2.225. [DOI] [PubMed] [Google Scholar]