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. 1969 Jul 1;42(1):202–220. doi: 10.1083/jcb.42.1.202

THE "VESICLE IN A BASKET"

A Morphological Study of the Coated Vesicle Isolated from the Nerve Endings of the Guinea Pig Brain, with Special Reference to the Mechanism of Membrane Movements

Toku Kanaseki 1, Ken Kadota 1
PMCID: PMC2107565  PMID: 4182372

Abstract

Five points are discussed regarding the vesicular structure isolated by fractionation techniques from the brain and liver of the guinea pig. 1. One type of vesicle, fixed by OsO4 and shown in thin sections, is identified with the coated vesicle that has been observed in many varieties of tissues. 2. The vesicle contained in a spherical polygonal "basketwork" shown by the negative-staining techniques is identical with the coated vesicle shown in sections. 3. Despite minute observation of this basketwork we could not confirm the existence of "hairlike projections" extending from the convex cytoplasmic surface of the vesicle. We are inclined to believe, therefore, that the hairlike projections are actually the superimposed visual images of the regular hexagons and pentagons of the network composing the basketwork. 4. We repeat the hypothesis originally advanced by Roth and Porter (1) that the "coating" of the coated vesicle plays a role in the mechanism of the infolding and fission of the membrane; we suggest that these events are caused by the transformation of the regular hexagons (of the coating) into regular pentagons. 5. Finally, we make a suggestion as to the nature of those vesicles which have on their surface subparticles which look like "elementary particles (2)."

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Selected References

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  1. ANDERSON E. OOCYTE DIFFERENTIATION AND VITELLOGENESIS IN THE ROACH PERIPLANETA AMERICANA. J Cell Biol. 1964 Jan;20:131–155. doi: 10.1083/jcb.20.1.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ANDRES K. H. MIKROPINOZYTOSE IM ZENTRALNERVENSYSTEM. Z Zellforsch Mikrosk Anat. 1964 Sep 17;64:63–73. [PubMed] [Google Scholar]
  3. BENNETT H. S., LUFT J. H. zeta-Collidine as a basis for buffering fixatives. J Biophys Biochem Cytol. 1959 Aug;6(1):113–114. doi: 10.1083/jcb.6.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BRANDT P. W. A consideration of the extraneous coats of the plasma membrane. Circulation. 1962 Nov;26:1075–1091. doi: 10.1161/01.cir.26.5.1075. [DOI] [PubMed] [Google Scholar]
  5. BRANDT P. W. A study of the mechanism of pinocytosis. Exp Cell Res. 1958 Oct;15(2):300–313. doi: 10.1016/0014-4827(58)90032-6. [DOI] [PubMed] [Google Scholar]
  6. BRIGHTMAN M. W., PALAY S. L. THE FINE STRUCTURE OF EPENDYMA IN THE BRAIN OF THE RAT. J Cell Biol. 1963 Nov;19:415–439. doi: 10.1083/jcb.19.2.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Beams H. W., Kessel R. G. The Golgi apparatus: structure and function. Int Rev Cytol. 1968;23:209–276. doi: 10.1016/s0074-7696(08)60273-9. [DOI] [PubMed] [Google Scholar]
  8. Bruni C., Porter K. R. The Fine Structure of the Parenchymal Cell of the Normal Rat Liver: I. General Observations. Am J Pathol. 1965 May;46(5):691–755. [PMC free article] [PubMed] [Google Scholar]
  9. Cardell R. R., Jr, Badenhausen S., Porter K. R. Intestinal triglyceride absorption in the rat. An electron microscopical study. J Cell Biol. 1967 Jul;34(1):123–155. doi: 10.1083/jcb.34.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Cunningham W. P., Morré D. J., Mollenhauer H. H. Structure of isolated plant Golgi apparatus revealed by negative staining. J Cell Biol. 1966 Feb;28(2):169–179. doi: 10.1083/jcb.28.2.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. DE ROBERTIS E., RODRIGUEZ DE LORES ARNAIZ G., SALGANICOFF L., PELLEGRINO DE IRALDI A., ZIEHER L. M. Isolation of synaptic vesicles and structural organization of the acetycholine system within brain nerve endings. J Neurochem. 1963 Apr;10:225–235. doi: 10.1111/j.1471-4159.1963.tb05038.x. [DOI] [PubMed] [Google Scholar]
  12. FARQUHAR M. G., PALADE G. E. Junctional complexes in various epithelia. J Cell Biol. 1963 May;17:375–412. doi: 10.1083/jcb.17.2.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. FARQUHAR M. G., WISSIG S. L., PALADE G. E. Glomerular permeability. I. Ferritin transfer across the normal glomerular capillary wall. J Exp Med. 1961 Jan 1;113:47–66. doi: 10.1084/jem.113.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. FAWCETT D. W. SURFACE SPECIALIZATIONS OF ABSORBING CELLS. J Histochem Cytochem. 1965 Feb;13:75–91. doi: 10.1177/13.2.75. [DOI] [PubMed] [Google Scholar]
  15. FERNANDEZ-MORAN H. Cell-membrane ultrastructure. Low-temperature electron microsopy and x-ray diffraction studies of lipoprotein components in lamellar systems. Circulation. 1962 Nov;26:1039–1065. doi: 10.1161/01.cir.26.5.1039. [DOI] [PubMed] [Google Scholar]
  16. Farquhar M. G., Palade G. E. Cell junctions in amphibian skin. J Cell Biol. 1965 Jul;26(1):263–291. doi: 10.1083/jcb.26.1.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Fleischer S., Fleischer B., Stoeckenius W. Fine structure of lipid-depleted mitochondria. J Cell Biol. 1967 Jan;32(1):193–208. doi: 10.1083/jcb.32.1.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Friend D. S., Farquhar M. G. Functions of coated vesicles during protein absorption in the rat vas deferens. J Cell Biol. 1967 Nov;35(2):357–376. doi: 10.1083/jcb.35.2.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. GRAY E. G. The granule cells, mossy synapses and Purkinje spine synapses of the cerebellum: light and electron microscope observations. J Anat. 1961 Jul;95:345–356. [PMC free article] [PubMed] [Google Scholar]
  20. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  21. Holtzman E., Novikoff A. B., Villaverde H. Lysosomes and GERL in normal and chromatolytic neurons of the rat ganglion nodosum. J Cell Biol. 1967 May;33(2):419–435. doi: 10.1083/jcb.33.2.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ito S. The enteric surface coat on cat intestinal microvilli. J Cell Biol. 1965 Dec;27(3):475–491. doi: 10.1083/jcb.27.3.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. KELLENBERGER E., RYTER A., SECHAUD J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol. 1958 Nov 25;4(6):671–678. doi: 10.1083/jcb.4.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Kadota K., Mori S., Imaizumi R. The properties of ATPase of synaptic vesicle fraction. J Biochem. 1967 Apr;61(4):424–432. doi: 10.1093/oxfordjournals.jbchem.a128565. [DOI] [PubMed] [Google Scholar]
  25. Kessel R. G. An electron microscope study of differentiation and growth in oocytes of Ophioderma panamensis. J Ultrastruct Res. 1968 Jan;22(1):63–89. doi: 10.1016/s0022-5320(68)90050-6. [DOI] [PubMed] [Google Scholar]
  26. Kessel R. G. Electron microscope studies on the origin and maturation of yolk in oocytes of the tunicate, Ciona intestinalis. Z Zellforsch Mikrosk Anat. 1966;71(4):525–544. doi: 10.1007/BF00349612. [DOI] [PubMed] [Google Scholar]
  27. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. MILLER F. Hemoglobin absorption by the cells of the proximal convoluted tubule in mouse kidney. J Biophys Biochem Cytol. 1960 Dec;8:689–718. doi: 10.1083/jcb.8.3.689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. MILLONIG G. A modified procedure for lead staining of thin sections. J Biophys Biochem Cytol. 1961 Dec;11:736–739. doi: 10.1083/jcb.11.3.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Marchesi V. T., Palade G. E. The localization of Mg-Na-K-activated adenosine triphosphatase on red cell ghost membranes. J Cell Biol. 1967 Nov;35(2):385–404. doi: 10.1083/jcb.35.2.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Maunsbach A. B. Absorption of I-125-labeled homologous albumin by rat kidney proximal tubule cells. A study of microperfused single proximal tubules by electron microscopic autoradiography and histochemistry. J Ultrastruct Res. 1966 Jun;15(3):197–241. doi: 10.1016/s0022-5320(66)80108-9. [DOI] [PubMed] [Google Scholar]
  32. Maunsbach A. B. Absorption of ferritin by rat kidney proximal tubule cells. Electron microscopic observations of the initial uptake phase in cells of microperfused single proximal tubules. J Ultrastruct Res. 1966 Sep;16(1):1–12. doi: 10.1016/s0022-5320(66)80019-9. [DOI] [PubMed] [Google Scholar]
  33. McIntosh J. R., Porter K. R. Microtubules in the spermatids of the domestic fowl. J Cell Biol. 1967 Oct;35(1):153–173. doi: 10.1083/jcb.35.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. PALAY S. L., KARLIN L. J. An electron microscopic study of the intestinal villus. II. The pathway of fat absorption. J Biophys Biochem Cytol. 1959 May 25;5(3):373–384. doi: 10.1083/jcb.5.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Palade G. E., Bruns R. R. Structural modulations of plasmalemmal vesicles. J Cell Biol. 1968 Jun;37(3):633–649. doi: 10.1083/jcb.37.3.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. ROSENBLUTH J., WISSIG S. L. THE DISTRIBUTION OF EXOGENOUS FERRITIN IN TOAD SPINAL GANGLIA AND THE MECHANISM OF ITS UPTAKE BY NEURONS. J Cell Biol. 1964 Nov;23:307–325. doi: 10.1083/jcb.23.2.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. ROTH T. F., PORTER K. R. YOLK PROTEIN UPTAKE IN THE OOCYTE OF THE MOSQUITO AEDES AEGYPTI. L. J Cell Biol. 1964 Feb;20:313–332. doi: 10.1083/jcb.20.2.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Ross R., Klebanoff S. J. Fine structural changes in uterine smooth muscle and fibroblasts in response to estrogen. J Cell Biol. 1967 Jan;32(1):155–167. doi: 10.1083/jcb.32.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. STASNY J. T., CRANE F. L. THE EFFECT OF SONIC OSCILLATION ON THE STRUCTURE AND FUNCTION OF BEEF HEART MITOCHONDRIA. J Cell Biol. 1964 Jul;22:49–62. doi: 10.1083/jcb.22.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Slautterback D. B. Coated vesicles in absorptive cells of Hydra. J Cell Sci. 1967 Dec;2(4):563–572. doi: 10.1242/jcs.2.4.563. [DOI] [PubMed] [Google Scholar]
  42. Turner F. R. An ultrastructural study of plant spermatogenesis. Spermatogenesis in Nitella. J Cell Biol. 1968 May;37(2):370–393. doi: 10.1083/jcb.37.2.370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Whittaker V. P., Michaelson I. A., Kirkland R. J. The separation of synaptic vesicles from nerve-ending particles ('synaptosomes'). Biochem J. 1964 Feb;90(2):293–303. doi: 10.1042/bj0900293. [DOI] [PMC free article] [PubMed] [Google Scholar]

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