Abstract
The soluble proteins in the hemolymph, the salivary gland, and the salivary secretion of fourth instar Chironomus tentans were examined by disc electrophoresis in acrylamide gels. Of the 11 protein fractions detected in buffered saline extracts of the gland, 10 are present also in the hemolymph. Amino acid isotope incorporation experiments indicate that the protein fractions shared by the salivary gland and the hemolymph are not synthesized in the gland but are synthesized in other larval tissues. Immunochemical studies show that most of these proteins eventually are secreted from the gland. The salivary gland in vivo and in vitro is active in de novo protein synthesis. The protein synthesized tends to form large molecular weight aggregates. As demonstrated by radioautography, at least 80% of this protein is secreted from the 30 large cells forming most of the gland. The proteins synthesized in the salivary gland cannot be detected in the hemolymph. The results of this investigation are consistent with a mechanism of secretion formation involving both de novo synthesis of some secretion proteins and the selective uptake, transport, and secretion of hemal proteins by the salivary gland.
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- BEERMANN W. [A Balbiani ring as locus of a salivary gland mutation]. Chromosoma. 1961;12:1–25. doi: 10.1007/BF00328911. [DOI] [PubMed] [Google Scholar]
- DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
- DEFRETIN R. Données nouvelles sur la sécretion salivaire des larves de Chironome. C R Hebd Seances Acad Sci. 1951 Jul 2;233(1):103–105. [PubMed] [Google Scholar]
- LAUFER H., NAKASE Y. SALIVARY GLAND SECRETION AND ITS RELATION TO CHROMOSOMAL PUFFING IN THE DIPTERAN, CHIRONOMUS THUMMI. Proc Natl Acad Sci U S A. 1965 Mar;53:511–516. doi: 10.1073/pnas.53.3.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- MANS R. J., NOVELLI G. D. A convenient, rapid and sensitive method for measuring the incorporation of radioactive amino acids into protein. Biochem Biophys Res Commun. 1960 Nov;3:540–543. doi: 10.1016/0006-291x(60)90171-6. [DOI] [PubMed] [Google Scholar]
- MECHELKE F. Reversible Strukturmodifikationen der Speicheldrüsenchromosomen von Acricotopus lucidus. Chromosoma. 1953;5(6-7):511–543. doi: 10.1007/BF01271500. [DOI] [PubMed] [Google Scholar]
- Moss B., Ingram V. M. The repression and induction by thyroxin of hemoglobin synthesis during amphibian metamorphosis. Proc Natl Acad Sci U S A. 1965 Sep;54(3):967–974. doi: 10.1073/pnas.54.3.967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ORNSTEIN L. DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY. Ann N Y Acad Sci. 1964 Dec 28;121:321–349. doi: 10.1111/j.1749-6632.1964.tb14207.x. [DOI] [PubMed] [Google Scholar]
- PELLING C. RIBONUKLEINSAEURE-SYNTHESE DER RIESENCHROMOSOMEN. AUTORADIOGRAPHISCHE UNTERSUCHUNGEN AN CHIRONOMUS TENTANS. Chromosoma. 1964 Apr 1;15:71–122. doi: 10.1007/BF00326915. [DOI] [PubMed] [Google Scholar]
- PERKOWSKA E. SOME CHARACTERISTICS OF THE SALIVARY GLAND SECRETION OF DROSOPHILA VIRILIS. Exp Cell Res. 1963 Nov;32:259–271. doi: 10.1016/0014-4827(63)90101-0. [DOI] [PubMed] [Google Scholar]
- ROTH T. F., PORTER K. R. YOLK PROTEIN UPTAKE IN THE OOCYTE OF THE MOSQUITO AEDES AEGYPTI. L. J Cell Biol. 1964 Feb;20:313–332. doi: 10.1083/jcb.20.2.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHNEIDER I. DIFFERENTIATION OF LARVAL DROSOPHILA EYE-ANTENNAL DISCS IN VITRO. J Exp Zool. 1964 Jun;156:91–103. doi: 10.1002/jez.1401560107. [DOI] [PubMed] [Google Scholar]
- SIEKEVITZ P. Uptake of radioactive alanine in vitro into the proteins of rat liver fractions. J Biol Chem. 1952 Apr;195(2):549–565. [PubMed] [Google Scholar]
- TELFER W. H. The route of entry and localization of blood proteins in the oocytes of saturniid moths. J Biophys Biochem Cytol. 1961 Apr;9:747–759. doi: 10.1083/jcb.9.4.747. [DOI] [PMC free article] [PubMed] [Google Scholar]