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. 1969 Jan 1;40(1):236–247. doi: 10.1083/jcb.40.1.236

ESTABLISHMENT OF A CLONAL STRAIN OF HEPATOMA CELLS WHICH SECRETE ALBUMIN

U Ingrid Richardson 1, Armen H Tashjian Jr 1, Lawrence Levine 1
PMCID: PMC2107590  PMID: 4177659

Abstract

A clonal strain of epithelial cells (designated MH1C1) has been established from the transplantable Morris hepatoma No. 7795. The cells have maintained distinctive morphology throughout more than 20 subcultures (split 1:5) at 2- to 4-week intervals in supplemented Ham's F 10 medium. They contain many highly refractile, round, cytoplasmic bodies which stain bright red with Oil Red O. The population doubling time was 2 wk when the clonal strain was first established. It has gradually decreased to 1 wk. The cells synthesize rat serum albumin and secrete it into the culture medium as determined immunologically by microcomplement fixation and double diffusion. Albumin secretion (3–6 µg albumin/mg cell nitrogen/24 hr) occurs throughout the logarithmic phase of cell proliferation and has not diminished during serial propagation since the strain was initiated 15 months ago.

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Selected References

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