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. 1988 Feb;170(2):1015–1017. doi: 10.1128/jb.170.2.1015-1017.1988

Confirmation of the Fur operator site by insertion of a synthetic oligonucleotide into an operon fusion plasmid.

S B Calderwood 1, J J Mekalanos 1
PMCID: PMC210760  PMID: 2828307

Abstract

We constructed a synthetic oligonucleotide corresponding to the previously proposed consensus binding site for the Fur protein, a central iron-regulatory protein of Escherichia coli. When this oligonucleotide was introduced at the start of transcription of an operon fusion between the ompF promoter and the lacZ structural gene, beta-galactosidase activity became iron regulated. This consensus sequence is sufficient to function as an operator site for the binding of Fur protein in vivo.

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Selected References

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