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. 1969 Jan 1;40(1):225–235. doi: 10.1083/jcb.40.1.225

HORMONE STORAGE GRANULES IN THE BEEF ANTERIOR PITUITARY

I. Isolation, Ultrastructure, and Some Biochemical Properties

Joseph T Tesar 1, Harold Koenig 1, Charles Hughes 1
PMCID: PMC2107604  PMID: 5782447

Abstract

A method is described for isolation of relatively large quantities of large and small hormone storage granules from the beef adenohypophysis. The hormone storage granules are highly purified, as indicated by ultrastructural and biochemical criteria. The average size of large granules is 400 mµ and of small granules is 220 mµ. The large granules contain growth hormone and prolactin; the small granules contain high concentrations of follicle-stimulating, luteinizing, and thyroid-stimulating hormones. An alkaline protease with a pH optimum of 8.3 is associated with the small granule fraction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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