Abstract
Potassium pyroantimonate added to fixative solutions has been used in tissue localization of sodium ions. The distribution and specificity of the resulting precipitate in rat kidney is described in this study. Two reproducible patterns of precipitate were obtained in control tissues. The first pattern, which occurred after fixation in solutions containing aldehyde, showed the precipitate to be mainly extracellular. The second pattern, showing the precipitate in both intracellular and extracellular locations, occurred after aldehyde fixation in those experimental situations favoring cellular swelling or after fixation with solutions containing osmium tetroxide. It appeared that sodium ions could move after fixation but that sodium pyroantimonate precipitate could not. Since model systems demonstrated that dense precipitate formed when potassium pyroantimonate was added to solutions containing certain biological amines or some divalent cations, it appeared likely that the reagent did not provide specific tissue localization for sodium ions.
Full Text
The Full Text of this article is available as a PDF (2.1 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CONWAY E. J., GEOGHEGAN H. Molecular concentration of kidney cortex slices. J Physiol. 1955 Nov 28;130(2):438–445. doi: 10.1113/jphysiol.1955.sp005417. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DEYRUP I. Reversal of fluid uptake by rat kidney slices immersed in isosmotic solutions in vitro. Am J Physiol. 1953 Dec;175(3):349–352. doi: 10.1152/ajplegacy.1953.175.3.349. [DOI] [PubMed] [Google Scholar]
- Grand R. J., Spicer S. S. Preliminary studies on the electron microscopic localization of sites of sodium transport in the human eccrine sweat gland. Bibl Paediatr. 1967;86:100–106. [PubMed] [Google Scholar]
- Kaye G. I., Cole J. D., Donn A. Electron microscopy: sodium localization in normal and ouabain-treated transporting cells. Science. 1965 Nov 26;150(3700):1167–1168. doi: 10.1126/science.150.3700.1167. [DOI] [PubMed] [Google Scholar]
- Kaye G. I., Wheeler H. O., Whitlock R. T., Lane N. Fluid transport in the rabbit gallbladder. A combined physiological and electron microscopic study. J Cell Biol. 1966 Aug;30(2):237–268. doi: 10.1083/jcb.30.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROBINSON J. R. Exchanges of water and ions by kidney slices determined by a balance method. J Physiol. 1961 Oct;158:449–460. doi: 10.1113/jphysiol.1961.sp006779. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Revel J. P., Karnovsky M. J. Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. J Cell Biol. 1967 Jun;33(3):C7–C12. doi: 10.1083/jcb.33.3.c7. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Siebert G., Langendorf H., Hannover R., Nitz-Litzow D., Pressman B. C., Moore C. Untersuchungen zur Rolle des Natrium-Stoffwechels im Zellkern der Rattenleber. Hoppe Seylers Z Physiol Chem. 1965;343(1):101–115. [PubMed] [Google Scholar]