Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 Feb;170(2):1018–1020. doi: 10.1128/jb.170.2.1018-1020.1988

Bacillus subtilis mutant deficient in the ability to produce the dipeptide antibiotic bacilysin: isolation and mapping of the mutation.

M D Hilton 1, N G Alaeddinoglu 1, A L Demain 1
PMCID: PMC210761  PMID: 2828308

Abstract

A Bacillus subtilis mutant which carries a lesion in a gene specific to the synthesis of the dipeptide antibiotic bacilysin was isolated. A derivative strain in which transposon Tn917 had inserted near the bacilysin lesion was isolated and used as the donor in PBS-1 transduction mapping experiments. The bac-1 locus was mapped between the ctrA and sacA loci, near 90% on the standard B. subtilis 168 chromosome map.

Full text

PDF
1018

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dedonder R. A., Lepesant J. A., Lepesant-Kejzlarová J., Billault A., Steinmetz M., Kunst F. Construction of a kit of reference strains for rapid genetic mapping in Bacillus subtilis 168. Appl Environ Microbiol. 1977 Apr;33(4):989–993. doi: 10.1128/aem.33.4.989-993.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Errington J. Efficient Bacillus subtilis cloning system using bacteriophage vector phi 105J9. J Gen Microbiol. 1984 Oct;130(10):2615–2628. doi: 10.1099/00221287-130-10-2615. [DOI] [PubMed] [Google Scholar]
  3. Hilton M. D., Alaeddinoglu N. G., Demain A. L. Synthesis of bacilysin by Bacillus subtilis branches from prephenate of the aromatic amino acid pathway. J Bacteriol. 1988 Jan;170(1):482–484. doi: 10.1128/jb.170.1.482-484.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hoch J. A., Barat M., Anagnostopoulos C. Transformation and transduction in recombination-defective mutants of Bacillus subtilis. J Bacteriol. 1967 Jun;93(6):1925–1937. doi: 10.1128/jb.93.6.1925-1937.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Perry D., Abraham E. P. Transport and metabolism of bacilysin and other peptides by suspensions of Staphylococcus aureus. J Gen Microbiol. 1979 Nov;115(1):213–221. doi: 10.1099/00221287-115-1-213. [DOI] [PubMed] [Google Scholar]
  6. Roscoe J., Abraham E. P. Experiments relating to the biosynthesis of bacilysin. Biochem J. 1966 Jun;99(3):793–800. doi: 10.1042/bj0990793. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Solomon L. R., Atherton J. C., Bobinski H., Green R. Effect of dietary sodium chloride and posture on plasma immunoreactive atrial natriuretic peptide concentrations in man. Clin Sci (Lond) 1987 Feb;72(2):201–208. doi: 10.1042/cs0720201. [DOI] [PubMed] [Google Scholar]
  8. Walker J. E., Abraham E. P. The structure of bacilysin and other products of Bacillus subtilis. Biochem J. 1970 Jul;118(4):563–570. doi: 10.1042/bj1180563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Youngman P., Perkins J. B., Losick R. A novel method for the rapid cloning in Escherichia coli of Bacillus subtilis chromosomal DNA adjacent to Tn917 insertions. Mol Gen Genet. 1984;195(3):424–433. doi: 10.1007/BF00341443. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES