Abstract
Confluent 3T3 cultures make and release into the medium a diffusible factor which sustains the state of contact inhibition of replication. Evidence is given that the factor is a specific and reversible inhibitor of the RNA synthesis which precedes cellular replication.
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- ALARCON R. A. ISOLATION OF ACROLEIN FROM INCUBATED MIXTURES OF SPERMINE WITH CALF SERUM AND ITS EFFECTS ON MAMMALIAN CELLS. Arch Biochem Biophys. 1964 Jul 20;106:240–242. doi: 10.1016/0003-9861(64)90183-3. [DOI] [PubMed] [Google Scholar]
- Boyle S. M., Cohen P. S. Putrescine-mediated degradation of ribonucleic acid in chloramphenicol-treated Escherichia coli. J Bacteriol. 1968 Oct;96(4):1266–1272. doi: 10.1128/jb.96.4.1266-1272.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cox R. P., Gesner B. M. Effect of simple sugars on the morphology and growth pattern of mammalian cell cultures. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1571–1579. doi: 10.1073/pnas.54.6.1571. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAVIES D. A. The identification of aldoheptose sugars. Biochem J. 1957 Oct;67(2):253–256. doi: 10.1042/bj0670253. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
- FISHER H. W., PUCK T. T., SATO G. Molecular growth requirements of single mammalian cells. III. Quantitative colonial growth of single S3 cells in a medium containing synthetic small molecular constituents and two purified protein fractions. J Exp Med. 1959 Jun 1;109(6):649–660. doi: 10.1084/jem.109.6.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fisher H. W., Yeh J. Contact inhibition in colony formation. Science. 1967 Feb 3;155(3762):581–582. doi: 10.1126/science.155.3762.581. [DOI] [PubMed] [Google Scholar]
- HAM R. G. Albumin replacement by fatty acids in clonal growth of mammalian cells. Science. 1963 May 17;140(3568):802–803. doi: 10.1126/science.140.3568.802. [DOI] [PubMed] [Google Scholar]
- HAM R. G. An improved nutrient solution for diploid Chinese hamster and human cell lines. Exp Cell Res. 1963 Feb;29:515–526. doi: 10.1016/s0014-4827(63)80014-2. [DOI] [PubMed] [Google Scholar]
- Holley R. W., Kiernan J. A. "Contact inhibition" of cell division in 3T3 cells. Proc Natl Acad Sci U S A. 1968 May;60(1):300–304. doi: 10.1073/pnas.60.1.300. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holmes R. Preparation from human serum of an alpha-one protein which induces the immediate growth of unadapted cells in vitro. J Cell Biol. 1967 Feb;32(2):297–308. doi: 10.1083/jcb.32.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVINE E. M., BECKER Y., BOONE C. W., EAGLE H. CONTACT INHIBITION, MACROMOLECULAR SYNTHESIS, AND POLYRIBOSOMES IN CULTURED HUMAN DIPLOID FIBROBLASTS. Proc Natl Acad Sci U S A. 1965 Feb;53:350–356. doi: 10.1073/pnas.53.2.350. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NOWELL P. C. Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes. Cancer Res. 1960 May;20:462–466. [PubMed] [Google Scholar]
- Puck T. T., Waldren C. A., Jones C. Mammalian cell growth proteins. I. Growth stimulation of fetuin. Proc Natl Acad Sci U S A. 1968 Jan;59(1):192–199. doi: 10.1073/pnas.59.1.192. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raina A., Cohen S. S. Polyamines and RNA synthesis in a polyauxotrophic strain of E. coli. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1587–1593. doi: 10.1073/pnas.55.6.1587. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raina A., Jansen M., Cohen S. S. Polyamines and the accumulation of ribonucleic acid in some polyauxotrophic strains of Escherichia coli. J Bacteriol. 1967 Nov;94(5):1684–1696. doi: 10.1128/jb.94.5.1684-1696.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TODARO G. J., GREEN H., GOLDBERG B. D. TRANSFORMATION OF PROPERTIES OF AN ESTABLISHED CELL LINE BY SV40 AND POLYOMA VIRUS. Proc Natl Acad Sci U S A. 1964 Jan;51:66–73. doi: 10.1073/pnas.51.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TODARO G. J., GREEN H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol. 1963 May;17:299–313. doi: 10.1083/jcb.17.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Todaro G. J., Lazar G. K., Green H. The initiation of cell division in a contact-inhibited mammalian cell line. J Cell Physiol. 1965 Dec;66(3):325–333. doi: 10.1002/jcp.1030660310. [DOI] [PubMed] [Google Scholar]