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. 1969 Feb 1;40(2):446–460. doi: 10.1083/jcb.40.2.446

EXTRACELLULAR MICROTUBULES

The Origin, Structure, and Attachment of Flagellar Hairs in Fucus and Ascophyllum Antherozoids

G Benjamin Bouck 1
PMCID: PMC2107633  PMID: 5812471

Abstract

Mastigonemes (Flimmer) from the sperm of Ascophyllum and Fucus were found to consist of a tripartite structure—a ca. 2000-A tapered basal region, a closed microtubular shaft, and a group of terminal filaments. Each of these regions appears to be constructed of globular subunits with a center-to-center distance of about 45 A. The mastigoneme microtubule is of smaller diameter (170–190 A) than cytoplasmic microtubules in these or other plant cells. During the initial stages of flagellar ontogeny, structures similar to mastigonemes (presumptive mastigonemes) are found within membrane-limited sacs in the cytoplasm or within the perinuclear space. Mastigonemes at this time are generally not found on the flagellar surface. Later, when the anterior flagellum acquires mastigonemes, the presumptive mastigonemes are absent from the cytoplasm. The regularity of attachment of mastigonemes to the flagellar surface suggests that specific attachment sites are constructed on the plasma membrane during flagellar ontogeny. No evidence for penetration of the mastigoneme through the plasma membrane was obtained. The origin and structure of mastigonemes are discussed in relation to reports of the origin and structure of other microtubular systems.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Behnke O., Forer A. Evidence for four classes of microtubules in individual cells. J Cell Sci. 1967 Jun;2(2):169–192. doi: 10.1242/jcs.2.2.169. [DOI] [PubMed] [Google Scholar]
  2. Borisy G. G., Taylor E. W. The mechanism of action of colchicine. Colchicine binding to sea urchin eggs and the mitotic apparatus. J Cell Biol. 1967 Aug;34(2):535–548. doi: 10.1083/jcb.34.2.535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradley D. E. The ultrastructure of the flagella of three chrysomonads with particular reference to the mastigonemes. Exp Cell Res. 1966 Jan;41(1):162–173. doi: 10.1016/0014-4827(66)90556-8. [DOI] [PubMed] [Google Scholar]
  4. GIBBS S. P. Chloroplast development in Ochromonas danica. J Cell Biol. 1962 Nov;15:343–361. doi: 10.1083/jcb.15.2.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GIBBS S. P. Nuclear envelope-chloroplast relationships in algae. J Cell Biol. 1962 Sep;14:433–444. doi: 10.1083/jcb.14.3.433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gall J. G. Microtubule fine structure. J Cell Biol. 1966 Dec;31(3):639–643. doi: 10.1083/jcb.31.3.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jenkins R. A. Fine structure of division in ciliate protozoa. I. Micronuclear mitosis in Blepharisma. J Cell Biol. 1967 Aug;34(2):463–481. doi: 10.1083/jcb.34.2.463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KERRIDGE D., HORNE R. W., GLAUERT A. M. Structural components of flagella from Salmonella typhimurium. J Mol Biol. 1962 Apr;4:227–238. doi: 10.1016/s0022-2836(62)80001-1. [DOI] [PubMed] [Google Scholar]
  9. LOWY J., HANSON J. ELECTRON MICROSCOPE STUDIES OF BACTERIAL FLAGELLA. J Mol Biol. 1965 Feb;11:293–313. doi: 10.1016/s0022-2836(65)80059-6. [DOI] [PubMed] [Google Scholar]
  10. Lowy J. Structure of the proximal ends of bacterial flagella. J Mol Biol. 1965 Nov;14(1):297–299. doi: 10.1016/s0022-2836(65)80251-0. [DOI] [PubMed] [Google Scholar]
  11. MANTON I., CLARKE B. Electron microscope observations on the spermatozoid of Fucus. Nature. 1950 Dec 9;166(4232):973–974. doi: 10.1038/166973a0. [DOI] [PubMed] [Google Scholar]
  12. Manton I. Further observations on the fine structure of Chrysochromulina chiton with special reference to the haptonema, 'peculiar' golgi structure and scale production. J Cell Sci. 1967 Jun;2(2):265–272. doi: 10.1242/jcs.2.2.265. [DOI] [PubMed] [Google Scholar]
  13. PEASE D. C. THE ULTRASTRUCTURE OF FLAGELLAR FIBRILS. J Cell Biol. 1963 Aug;18:313–326. doi: 10.1083/jcb.18.2.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Phillips D. M. Substructure of flagellar tubules. J Cell Biol. 1966 Dec;31(3):635–638. doi: 10.1083/jcb.31.3.635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pickett-Heaps J. D., Northcote D. H. Organization of microtubules and endoplasmic reticulum during mitosis and cytokinesis in wheat meristems. J Cell Sci. 1966 Mar;1(1):109–120. doi: 10.1242/jcs.1.1.109. [DOI] [PubMed] [Google Scholar]
  16. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ringo D. L. The arrangement of subunits in flagellar fibers. J Ultrastruct Res. 1967 Feb;17(3):266–277. doi: 10.1016/s0022-5320(67)80048-0. [DOI] [PubMed] [Google Scholar]
  18. SHELLABARGER C. J., LASIEWSKI R. C., HYNCIK G. E. Thyroid function in active, sleeping and torpid hummingbirds. Nature. 1961 Sep 23;191:1318–1318. doi: 10.1038/1911318a0. [DOI] [PubMed] [Google Scholar]
  19. Shelanski M. L., Taylor E. W. Isolation of a protein subunit from microtubules. J Cell Biol. 1967 Aug;34(2):549–554. doi: 10.1083/jcb.34.2.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Van Iterson W., Hoeniger J. F., Van Zanten E. N. Basal bodies of bacterial flagella in Proteus mirabilis. I. Electron microscopy of sectioned material. J Cell Biol. 1966 Dec;31(3):585–601. doi: 10.1083/jcb.31.3.585. [DOI] [PMC free article] [PubMed] [Google Scholar]

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