Skip to main content
PMC home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1969 Aug 1;42(2):460–468. doi: 10.1083/jcb.42.2.460

III. DECREASE OF LABELED DNA IN CELLS OF THE ADRENAL MEDULLA AFTER INTERMITTENT EXPOSURE TO COLD

S R Pelc 1, Maria Pia Viola-Magni 1
PMCID: PMC2107685  PMID: 5792334

Abstract

Italico rats were injected with thymidine-3H 6 hr after the end of 300 hr of intermittent cold treatment. This plan of experiment ensured replacement in the adrenal medulla of lost DNA which is specifically sensitive to cold treatment and has a labeling index sufficiently high for statistical evaluation. The labeling index in the adrenal medulla decreases to one-half of the initial value within 10 days in animals subjected to further intermittent cold treatment and within 32 days in animals kept at room temperature. The very low mitotic index and the absence of doubling of the labeling index show that the observed labeling cannot be ascribed to pre-mitotic DNA synthesis. The concept of metabolic DNA adequately explains the findings.

Full Text

The Full Text of this article is available as a PDF (504.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arold R., Sandritter W. Zytophotometrische Bestimmungen des DNS-Gehaltes von Zellkernen des Nebennierenmarkes, der Nebennierenrinde und der Schilddrüse unter verschiedenen experimentellen Bedingungen. Histochemie. 1967;10(1):88–97. doi: 10.1007/BF00304377. [DOI] [PubMed] [Google Scholar]
  2. Cohn N. S., van Duijn P. Constancy of DNA content in adrenal medulla nuclei of cold-treated rats. J Cell Biol. 1967 May;33(2):349–354. doi: 10.1083/jcb.33.2.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GALL J. G., JOHNSON W. W. Is there "metabolic" DNA in the mouse seminal vesicle? J Biophys Biochem Cytol. 1960 Jul;7:657–666. doi: 10.1083/jcb.7.4.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. LASNITZKI I., PELC S. R. Effect of 20-methylcholanthrene on DNA synthesis in mouse prostates grown in vitro. Exp Cell Res. 1957 Aug;13(1):140–146. doi: 10.1016/0014-4827(57)90055-1. [DOI] [PubMed] [Google Scholar]
  5. LEBLOND C. P., WALKER B. E. Renewal of cell populations. Physiol Rev. 1956 Apr;36(2):255–276. doi: 10.1152/physrev.1956.36.2.255. [DOI] [PubMed] [Google Scholar]
  6. MESSIER B., LEBLOND C. P. Cell proliferation and migration as revealed by radioautography after injection of thymidine-H3 into male rats and mice. Am J Anat. 1960 May;106:247–285. doi: 10.1002/aja.1001060305. [DOI] [PubMed] [Google Scholar]
  7. Malvaldi G., Mencacci P., Viola-Magni M. P. Mitoses in the adrenal medullary cells. Experientia. 1968 May 15;24(5):475–476. doi: 10.1007/BF02144402. [DOI] [PubMed] [Google Scholar]
  8. PELC S. R., GAHAN P. B. Incorporation of labelled thymidine in the seminal vesicle of the mouse. Nature. 1959 Jan 31;183(4657):335–336. doi: 10.1038/183335a0. [DOI] [PubMed] [Google Scholar]
  9. PELC S. R. Incorporation of tritiated thymidine in various organs of the mouse. Nature. 1962 Feb 24;193:793–795. doi: 10.1038/193793a0. [DOI] [PubMed] [Google Scholar]
  10. PELC S. R., LA COUR L. F. The incorporation of H3-thymidine in newly differentiated nuclei of roots of Vicia faba. Experientia. 1959 Apr 15;15(4):131–133. doi: 10.1007/BF02165521. [DOI] [PubMed] [Google Scholar]
  11. PELC S. R. LABELLING OF DNA AND CELL DIVISION IN SO CALLED NON-DIVIDING TISSUES. J Cell Biol. 1964 Jul;22:21–28. doi: 10.1083/jcb.22.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. PELC S. R. Nuclear uptake of labelled adenine in the seminal vesicle of the mouse. Exp Cell Res. 1958 Apr;14(2):301–315. doi: 10.1016/0014-4827(58)90188-5. [DOI] [PubMed] [Google Scholar]
  13. Pelc S. R. Turnover of DNA and function. Nature. 1968 Jul 13;219(5150):162–163. doi: 10.1038/219162a0. [DOI] [PubMed] [Google Scholar]
  14. Stroun M., Charles P., Anker P., Pelc S. R. Metabolic DNA in heart and skeletal muscle and in the intestine of mice. Nature. 1967 Nov 18;216(5116):716–717. doi: 10.1038/216716a0. [DOI] [PubMed] [Google Scholar]
  15. Viola-Magni M. P. A radioautographic study with H3-thymidine on adrenal medulla nuclei of rats intermittently exposed to cold. J Cell Biol. 1966 Jan;28(1):9–19. doi: 10.1083/jcb.28.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Viola-Magni M. P. Changes in the DNA content of adrenal medulla nuclei of rats intermittently exposed to cold. J Cell Biol. 1965 Jun;25(3):415–433. doi: 10.1083/jcb.25.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES