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. 1969 Apr 1;41(1):251–268. doi: 10.1083/jcb.41.1.251

PANETH AND GOBLET CELL RENEWAL IN MOUSE DUODENAL CRYPTS

W David Troughton 1, Jerry S Trier 1
PMCID: PMC2107723  PMID: 5775788

Abstract

Proliferation of Paneth and goblet cells of mouse duodenal crypts was studied by high resolution light microscope radioautography. In one group of mice, blood levels of thymidine-3H were sustained for up to 12 hr by repeated injections of isotope to facilitate identification of proliferating cells. In these animals, many goblet cell nuclei incorporated thymidine-3H whereas only 1 of 6261 tabulated Paneth cells was labeled. Cells intermediate in structure between undifferentiated and goblet cells and between undifferentiated and Paneth cells were identified and their light and electron microscopic features are described. A significant number of these "intermediate" cells incorporated thymidine-3H into their nuclei. Another group of mice received a single injection of thymidine-3H. These animals were killed 4 hr to 29 days after isotope administration. Goblet cells and intermediate cells with labeled nuclei were identified 4 hr after thymidine-3H but could not be seen after 15 days. In contrast, Paneth cells with labeled nuclei were not observed until 24 hr after thymidine-3H but were still present at 29 days, long after labeled undifferentiated, goblet, and intermediate cells had disappeared. We conclude that differentiated Paneth cells in mouse duodenum do not normally proliferate, but, instead, arise by differentiation from undifferentiated crypt cells or from intermediate cells. Moreover, once formed, Paneth cells persist in crypts for a prolonged period. In contrast, intermediate cells and crypt goblet cells proliferate actively and are less stable cell populations than differentiated Paneth cells. The precise function of the intermediate cells is not known, but they may represent transition forms between undifferentiated cells and the more matrure secretory cells. Damage of crypt epithelial cells, thought to be due to radiation effects, was evident in both groups of mice.

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Selected References

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  1. CARO L. G., VAN TUBERGEN R. P., KOLB J. A. High-resolution autoradiography. I. Methods. J Cell Biol. 1962 Nov;15:173–188. doi: 10.1083/jcb.15.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Deschner E. E. Observations on the Paneth cell in human ileum. Exp Cell Res. 1967 Sep;47(3):624–628. doi: 10.1016/0014-4827(67)90021-3. [DOI] [PubMed] [Google Scholar]
  3. HALLY A. D. The fine structure of the Paneth cell. J Anat. 1958 Apr;92(2):268–277. [PMC free article] [PubMed] [Google Scholar]
  4. Hughes W. L., Bond V. P., Brecher G., Cronkite E. P., Painter R. B., Quastler H., Sherman F. G. CELLULAR PROLIFERATION IN THE MOUSE AS REVEALED BY AUTORADIOGRAPHY WITH TRITIATED THYMIDINE. Proc Natl Acad Sci U S A. 1958 May;44(5):476–483. doi: 10.1073/pnas.44.5.476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hugon J., Borgers M. Ultrastructural and cytochemical studies on karyolytic bodies in the epithelium of the duodenal crypts of whole body x-irradiated mice. Lab Invest. 1966 Oct;15(10):1528–1543. [PubMed] [Google Scholar]
  6. LEBLOND C. P., MESSIER B. Renewal of chief cells and goblet cells in the small intestine as shown by radioautography after injection of thymidine-H3 into mice. Anat Rec. 1958 Nov;132(3):247–259. doi: 10.1002/ar.1091320303. [DOI] [PubMed] [Google Scholar]
  7. LEBLOND C. P., WALKER B. E. Renewal of cell populations. Physiol Rev. 1956 Apr;36(2):255–276. doi: 10.1152/physrev.1956.36.2.255. [DOI] [PubMed] [Google Scholar]
  8. LIPKIN M., SHERLOCK P., BELL B. CELL PROLIFERATION KINETICS IN THE GASTROINTESTINAL TRACT OF MAN. II. CELL RENEWAL IN STOMACH, ILEUM, COLON, AND RECTUM. Gastroenterology. 1963 Dec;45:721–729. [PubMed] [Google Scholar]
  9. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. RUBINI J. R., CRONKITE E. P., BOND V. P., FLIEDNER T. M. The metabolism and fate of tritiated thymidine in man. J Clin Invest. 1960 Jun;39:909–918. doi: 10.1172/JCI104111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Spicer S. S., Staley M. W., Wetzel M. G., Wetzel B. K. Acid mucosubstance and basic protein in mouse Paneth cells. J Histochem Cytochem. 1967 Apr;15(4):225–242. doi: 10.1177/15.4.225. [DOI] [PubMed] [Google Scholar]
  13. Staley M. W., Trier J. S. Morphologic heterogeneity of mouse Paneth cell granules before and after secretory stimulation. Am J Anat. 1965 Nov;117(3):365–383. doi: 10.1002/aja.1001170305. [DOI] [PubMed] [Google Scholar]
  14. TRIER J. S. STUDIES ON SMALL INTESTINAL CRYPT EPITHELIUM. I. THE FINE STRUCTURE OF THE CRYPT EPITHELIUM OF THE PROXIMAL SMALL INTESTINE OF FASTING HUMANS. J Cell Biol. 1963 Sep;18:599–620. doi: 10.1083/jcb.18.3.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thrasher J. D., Greulich R. C. The duodenal progenitor population. 3. The progenitor cell cycle of principal, goblet and paneth cells. J Exp Zool. 1966 Feb;161(1):9–19. doi: 10.1002/jez.1401610103. [DOI] [PubMed] [Google Scholar]
  16. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium. J Biophys Biochem Cytol. 1958 Nov 25;4(6):727–730. doi: 10.1083/jcb.4.6.727. [DOI] [PMC free article] [PubMed] [Google Scholar]

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