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. 1969 Apr 1;41(1):1–20. doi: 10.1083/jcb.41.1.1

RESOLUTION IN ELECTRON MICROSCOPE RADIOAUTOGRAPHY

M M Salpeter 1, L Bachmann 1, E E Salpeter 1
PMCID: PMC2107734  PMID: 5775785

Abstract

An analysis of grain distributions around a radioactive line source (consisting of polystyrene-3H) showed that the shape of the distribution was independent of the factors that influence resolution, i.e. section and emulsion thickness, silver halide crystal, and developed grain size. These factors did effect the spread of the distribution, however, and thus the distance from the line source within which 50% of the total developed grains fell. We called this distance "half distance" (HD) and determined it for a variety of specimens. When grain distributions were normalized in units of HD, one could plot universal grain distributions for specimens with radioactive sources of various shapes. The use of HD and the universal curves in interpreting radioautograms is discussed.

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Selected References

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  1. BACHMANN L., SALPETER M. M. AUTORADIOGRAPHY WITH THE ELECTRON MICROSCOPE; A QUANTITATIVE EVALUATION. Lab Invest. 1965 Jun;14:1041–1053. [PubMed] [Google Scholar]
  2. BACHMANN L., SITTE P. Dickenbestimmung nach Tolansky an Ultradünnschnitten. Mikroskopie. 1959 Feb;13(9-10):289–304. [PubMed] [Google Scholar]
  3. Bachmann L., Salpeter M. M. Absolute sensitivity of electron microscope radioautography. J Cell Biol. 1967 May;33(2):299–305. doi: 10.1083/jcb.33.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bachmann L., Salpeter M. M., Salpeter E. E. Das Auflösungsvermögen elektronenmikroskopischer Autoradiographien. Histochemie. 1968;15(3):234–250. doi: 10.1007/BF00305888. [DOI] [PubMed] [Google Scholar]
  5. CARO L. G. High-resolution autoradiogaphy. II. The problem of resolution. J Cell Biol. 1962 Nov;15:189–199. doi: 10.1083/jcb.15.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. CARO L. G., VAN TUBERGEN R. P., KOLB J. A. High-resolution autoradiography. I. Methods. J Cell Biol. 1962 Nov;15:173–188. doi: 10.1083/jcb.15.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Caro L. G., Schnös M. Tritium and Phosphorus-32 in High-Resolution Autoradiography. Science. 1965 Jul 2;149(3679):60–62. doi: 10.1126/science.149.3679.60. [DOI] [PubMed] [Google Scholar]
  8. Coimbra A., Leblond C. P. Sites of glycogen synthesis in rat liver cells as shown by electron microscope radioautography after administration of glucose-H3. J Cell Biol. 1966 Jul;30(1):151–175. doi: 10.1083/jcb.30.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hülser D. F., Rajewsky M. F. Characteristics of three nuclear emulsions for autoradiography at the electron microscope. Biophysik. 1966;3(2):123–130. doi: 10.1007/BF01191605. [DOI] [PubMed] [Google Scholar]
  10. LIQUIER-MILWARD J. Electron microscopy and radioautography as coupled techniques in tracer experiments. Nature. 1956 Mar 31;177(4509):619–619. doi: 10.1038/177619a0. [DOI] [PubMed] [Google Scholar]
  11. PEACHEY L. D. Thin sections. I. A study of section thickness and physical distortion produced during microtomy. J Biophys Biochem Cytol. 1958 May 25;4(3):233–242. doi: 10.1083/jcb.4.3.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SALPETER M. M., BACHMANN L. AUTORADIOGRAPHY WITH THE ELECTRON MICROSCOPE. A PROCEDURE FOR IMPROVING RESOLUTION, SENSITIVITY, AND CONTRAST. J Cell Biol. 1964 Aug;22:469–477. doi: 10.1083/jcb.22.2.469. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. STEVENS G. W. W. Radioactive microphotographs for resolution testing in autoradiography. Br J Radiol. 1950 Dec;23(276):723–730. doi: 10.1259/0007-1285-23-276-723. [DOI] [PubMed] [Google Scholar]

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