Abstract
The rat liver nucleolus, after fragmentation induced by ethionine treatment, has been found to undergo complete reformation by adenine in the presence of a dose of cycloheximide sufficient to cause inhibition of protein synthesis by 90–95%. In contrast, actinomycin D given along with adenine was followed by the appearance of a small compact mass containing only the fibrillar component with no evident granules. This structure resembled pseudonucleoli seen in the anucleolate mutant of Xenopus laevis or in certain early stages of amphibian oocytes. Actinomycin D administered 2 hr after adenine induced a segregation of the fibrillar and granular components of nucleoli similar to that induced in the normal nucleolus. The implications of these findings in relation to nucleolar organization are briefly discussed.
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Selected References
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- Jones K. W. The role of the nucleolus in the formation of ribosomes. J Ultrastruct Res. 1965 Oct;13(3):257–262. doi: 10.1016/s0022-5320(65)80074-0. [DOI] [PubMed] [Google Scholar]
- KARNOVSKY M. J. Simple methods for "staining with lead" at high pH in electron microscopy. J Biophys Biochem Cytol. 1961 Dec;11:729–732. doi: 10.1083/jcb.11.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miyai K., Steiner J. W. Fine structure of interphase liver cell nuclei in acute ethionine intoxication. Lab Invest. 1967 May;16(5):677–692. [PubMed] [Google Scholar]
- REYNOLDS R. C., MONTGOMERY P. O., HUGHES B. NUCLEOLAR "CAPS" PRODUCED BY ACTINOMYCIN D. Cancer Res. 1964 Aug;24:1269–1277. [PubMed] [Google Scholar]
- SCHOEFL G. I. THE EFFECT OF ACTINOMYCIN D ON THE FINE STRUCTURE OF THE NUCLEOLUS. J Ultrastruct Res. 1964 Apr;10:224–243. doi: 10.1016/s0022-5320(64)80007-1. [DOI] [PubMed] [Google Scholar]
- STEVENS B. J. THE EFFECT OF ACTINOMYCIN D ON NUCLEOLAR AND NUCLEAR FINE STRUCTURE IN THE SALIVARY GLAND CELL OF HIRONOMUS THUMMI. J Ultrastruct Res. 1964 Oct;11:329–353. doi: 10.1016/s0022-5320(64)90037-1. [DOI] [PubMed] [Google Scholar]
- Shinozuka H., Goldblatt P. J., Farber E. The disorganization of hepatic cell nucleoli induced by ethionine and its reversal by adenine. J Cell Biol. 1968 Feb;36(2):313–328. doi: 10.1083/jcb.36.2.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simard R., Bernhard W. Le phénomène de la ségrégation nucléolaire: spécificité d'action de certains antimétabolites. Int J Cancer. 1966 Sep 15;1(5):463–479. doi: 10.1002/ijc.2910010506. [DOI] [PubMed] [Google Scholar]
- Smuckler E. A., Benditt E. P. The early effects of actinomycin on rat liver. Changes in ribosomes and polysomes. Lab Invest. 1965 Oct;14(10):1699–1709. [PubMed] [Google Scholar]
- Stewart G. A., Farber E. The rapid acceleration of hepatic nuclear ribonucleic acid breakdown by actinomycin but not by ethionine. J Biol Chem. 1968 Sep 10;243(17):4479–4485. [PubMed] [Google Scholar]
- VILLA-TREVINO S., SHULL K. H., FARBER E. The role of adenosine triphosphate deficiency in ethionine-induced inhibition of protein synthesis. J Biol Chem. 1963 May;238:1757–1763. [PubMed] [Google Scholar]
- Verbin R. S., Farber E. Effect of cycloheximide on the cell cycle of the crypts of the small intestine of the rat. J Cell Biol. 1967 Dec;35(3):649–658. doi: 10.1083/jcb.35.3.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Villa-Trevino S., Shull K. H., Farber E. The inhibition of liver ribonucleic acid synthesis by ethionine. J Biol Chem. 1966 Oct 25;241(20):4670–4674. [PubMed] [Google Scholar]
- Warner J. R., Girard M., Latham H., Darnell J. E. Ribosome formation in HeLa cells in the absence of protein synthesis. J Mol Biol. 1966 Aug;19(2):373–382. doi: 10.1016/s0022-2836(66)80011-6. [DOI] [PubMed] [Google Scholar]