Abstract
Human and rabbit red blood cells, separated into "young" and "old" age groups by differential flotation on phthalate esters, were fixed with glutaraldehyde and labeled with colloidal ferric oxide. Electron micrographs of thin sections of young cells showed a uniform and dense depostion of positive iron particles. Old cells showed particles deposited irregularly, leaving unlabeled gaps on the membrane surface. Red cells incubated with 10 units/ml receptor-destroying enzyme (RDE) demonstrate a reduced labeling, similar to that of old cells. After neuraminic acid had been removed from red cells by 20 units/ml RDE, no iron particles were found on membrane surfaces. The different labeling of young, old, and RDE-treated human and rabbit red cells was correlated with their electric mobility and agglutinability by poly-L-lysine. The contradiction between the apparent similarity in charge density of human and rabbit red cells as estimated by density of iron particles and the markedly lower electric mobility of rabbit red cells is discussed.
Full Text
The Full Text of this article is available as a PDF (516.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Benedetti E. L., Emmelot P. Studies on plasma membranes. IV. The ultrastructural localization and content of sialic acid in plasma membranes isolated from rat liver and hepatoma. J Cell Sci. 1967 Dec;2(4):499–512. doi: 10.1242/jcs.2.4.499. [DOI] [PubMed] [Google Scholar]
- DANON D., MARIKOVSKY V. DETERMINATION OF DENSITY DISTRIBUTION OF RED CELL POPULATION. J Lab Clin Med. 1964 Oct;64:668–674. [PubMed] [Google Scholar]
- Danon D., Marikovsky Y., Kohn A. Red cell agglutination kinetics: a method for automatic recording with the fragiligraph. Experientia. 1969 Jan 15;25(1):104–106. doi: 10.1007/BF01903926. [DOI] [PubMed] [Google Scholar]
- EYLAR E. H., MADOFF M. A., BRODY O. V., ONCLEY J. L. The contribution of sialic acid to the surface charge of the erythrocyte. J Biol Chem. 1962 Jun;237:1992–2000. [PubMed] [Google Scholar]
- GLAESER R. M., MEL H. C. THE ELECTROPHORETIC BEHAVIOR OF OSMIUM TETROXIDE-FIXED AND POTASSIUM PERMANGANATE-FIXED RAT ERYTHROCYTES. Biochim Biophys Acta. 1964 May 25;79:606–617. doi: 10.1016/0926-6577(64)90227-x. [DOI] [PubMed] [Google Scholar]
- GOTTSCHALK A., LIND P. E. Product of interaction between influenza virus enzyme and ovomucin. Nature. 1949 Aug 6;164(4162):232–232. doi: 10.1038/164232a0. [DOI] [PubMed] [Google Scholar]
- Gasic G. J., Berwick L., Sorrentino M. Positive and negative colloidal iron as cell surface electron stains. Lab Invest. 1968 Jan;18(1):63–71. [PubMed] [Google Scholar]
- Haydon D. A., Seaman G. V. Electrokinetic studies on the ultrastructure of the human erythrocyte. I. Electrophoresis at high ionic strengths--the cell as a polyanion. Arch Biochem Biophys. 1967 Oct;122(1):126–136. doi: 10.1016/0003-9861(67)90131-2. [DOI] [PubMed] [Google Scholar]
- Haydon D. A., Seaman G. V. Electrokinetic studies on the ultrastructure of the human erythrocyte. I. Electrophoresis at high ionic strengths--the cell as a polyanion. Arch Biochem Biophys. 1967 Oct;122(1):126–136. doi: 10.1016/0003-9861(67)90131-2. [DOI] [PubMed] [Google Scholar]
- Marikovsky Y., Danon D., Katchalsky A. Agglutination by polylysine of young and old red blood cells. Biochim Biophys Acta. 1966 Jul 27;124(1):154–159. [PubMed] [Google Scholar]
- SACHTLEBEN P., RUHENSTROTH-BAUER G. Agglutination and the electrical surface potential of red blood cells. Nature. 1961 Dec 9;192:982–983. doi: 10.1038/192982b0. [DOI] [PubMed] [Google Scholar]
- STONE J. D., ADA G. L. Electrophoretic studies of virus-red cell interaction: relationship between agglutinability and electrophoretic mobility. Br J Exp Pathol. 1952 Oct;33(5):428–439. [PMC free article] [PubMed] [Google Scholar]
- Yaari A. Mobility of human red blood cells of different age groups in an electric field. Blood. 1969 Feb;33(2):159–163. [PubMed] [Google Scholar]